Phospho-Cofilin (Ser3) Antibody | Affinity Biosciences

Product:	Phospho-Cofilin (Ser3) Antibody
Catalog:	AF3232
Description:	Rabbit polyclonal antibody to Phospho-Cofilin (Ser3)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	19kDa; 19kD(Calculated).
Uniprot:	P23528
RRID:	AB_2834658

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-Cofilin (Ser3) Antibody detects endogenous levels of Cofilin only when phosphorylated at Serine 3.

RRID:

AB_2834658
Cite Format: Affinity Biosciences Cat# AF3232, RRID:AB_2834658.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; Cofilin-1; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18;

Immunogens

Immunogen:

Uniprot:

P23528(COF1_HUMAN) >>Visit HPA database.

Gene(ID):

CFL1(1072)

Expression:

P23528 COF1_HUMAN:

Widely distributed in various tissues.

Description:

cofilin 1 a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described.

Sequence:

P23528 COF1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23528 As Substrate

P23528

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S3	Phosphorylation	P53671 (LIMK2) , P53667 (LIMK1) , Q13418 (ILK) , Q15139 (PRKD1) , Q96S53 (TESK2) , Q15569 (TESK1) , Q7Z2Y5 (NRK)	Uniprot
S8	Phosphorylation		Uniprot
K13	Acetylation		Uniprot
K13	Sumoylation		Uniprot
K13	Ubiquitination		Uniprot
K19	Acetylation		Uniprot
K19	Methylation		Uniprot
K19	Sumoylation		Uniprot
K19	Ubiquitination		Uniprot
K22	Ubiquitination		Uniprot
S23	Phosphorylation	P17252 (PRKCA)	Uniprot
S24	Phosphorylation	P17252 (PRKCA)	Uniprot
T25	Phosphorylation		Uniprot
K30	Acetylation		Uniprot
K30	Sumoylation		Uniprot
K30	Ubiquitination		Uniprot
K31	Acetylation		Uniprot
K33	Acetylation		Uniprot
K34	Sumoylation		Uniprot
K34	Ubiquitination		Uniprot
C39	S-Nitrosylation		Uniprot
S41	Phosphorylation		Uniprot
K44	Acetylation		Uniprot
K44	Sumoylation		Uniprot
K44	Ubiquitination		Uniprot
K45	Acetylation		Uniprot
K45	Sumoylation		Uniprot
K45	Ubiquitination		Uniprot
K53	Sumoylation		Uniprot
K53	Ubiquitination		Uniprot
T63	Phosphorylation		Uniprot
Y68	Phosphorylation	P12931 (SRC)	Uniprot
T70	Phosphorylation		Uniprot
K73	Acetylation		Uniprot
K73	Ubiquitination		Uniprot
K78	Acetylation		Uniprot
K78	Ubiquitination		Uniprot
C80	S-Nitrosylation		Uniprot
Y82	Phosphorylation		Uniprot
Y85	Phosphorylation		Uniprot
T88	Phosphorylation		Uniprot
Y89	Phosphorylation		Uniprot
T91	Phosphorylation		Uniprot
K92	Acetylation		Uniprot
K92	Ubiquitination		Uniprot
S94	Phosphorylation		Uniprot
K95	Ubiquitination		Uniprot
K96	Acetylation		Uniprot
K96	Methylation		Uniprot
K96	Ubiquitination		Uniprot
S108	Phosphorylation		Uniprot
K112	Sumoylation		Uniprot
K112	Ubiquitination		Uniprot
K114	Acetylation		Uniprot
K114	Sumoylation		Uniprot
K114	Ubiquitination		Uniprot
Y117	Phosphorylation		Uniprot
S119	Phosphorylation		Uniprot
S120	Phosphorylation		Uniprot
K121	Acetylation		Uniprot
K121	Sumoylation		Uniprot
K121	Ubiquitination		Uniprot
K125	Acetylation		Uniprot
K126	Acetylation		Uniprot
K127	Acetylation		Uniprot
K127	Ubiquitination		Uniprot
T129	Phosphorylation		Uniprot
K132	Acetylation		Uniprot
K132	Sumoylation		Uniprot
K132	Ubiquitination		Uniprot
C139	S-Nitrosylation		Uniprot
Y140	Phosphorylation		Uniprot
K144	Acetylation		Uniprot
K144	Ubiquitination		Uniprot
C147	S-Nitrosylation		Uniprot
K152	Ubiquitination		Uniprot
S156	Phosphorylation		Uniprot
S160	Phosphorylation		Uniprot
K164	Sumoylation		Uniprot
K164	Ubiquitination		Uniprot

Research Backgrounds

P23528_COF1_HUMAN

Function:

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration (By similarity).

PTMs:

Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.

Subcellular Location:

Nucleus matrix. Cytoplasm>Cytoskeleton. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium.
Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

Tissue Specificity:

Widely distributed in various tissues.

Subunit Structure:

Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods.

Family&Domains:

Belongs to the actin-binding proteins ADF family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Organismal Systems > Development > Axon guidance. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

References

1). Neochlorogenic acid anchors MCU-based calcium overload for cancer therapy. Food & Function, 2021 (PubMed: 34672304) [IF=6.1]

2). Baicalin Attenuates Panton-Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells. International journal of molecular sciences, 2023 (PubMed: 37833969) [IF=5.6]

3). Function of connexin 43 and RhoA/LIMK2/Cofilin signaling pathway in transient changes of contraction and dilation of human umbilical arterial smooth muscle cells. The International Journal of Biochemistry & Cell Biology, 2022 (PubMed: 36330887) [IF=4.0]

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Phospho-Cofilin (Ser3) Antibody - #AF3232