Phospho-BRCA2 (Ser2095) Antibody - #AF7317
Product: | Phospho-BRCA2 (Ser2095) Antibody |
Catalog: | AF7317 |
Description: | Rabbit polyclonal antibody to Phospho-BRCA2 (Ser2095) |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Bovine |
Mol.Wt.: | 384kDa; 384kD(Calculated). |
Uniprot: | P51587 |
RRID: | AB_2843757 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7317, RRID:AB_2843757.
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BRCA 2; BRCA1/BRCA2 containing complex subunit 2; Brca2; BRCA2, DNA repair associated; BRCA2_HUMAN; BRCC 2; BRCC2; Breast and ovarian cancer susceptibility gene early onset; breast and ovarian cancer susceptibility protein 2; Breast cancer 2 early onset; Breast Cancer 2 tumor suppressor; Breast cancer susceptibility protein BRCA2; Breast cancer type 2 susceptibility protein; BROVCA2; FACD; FAD 1; FAD; FAD1; FANCB; FANCD 1; FANCD; FANCD1; FANCD1 gene; Fanconi anemia complementation group D1; Fanconi anemia group D1 protein; GLM3; mutant BRCA2; OTTHUMP00000018803; OTTHUMP00000042401; PNCA2; XRCC11;
Immunogens
Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
- P51587 BRCA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPIGSKERPTFFEIFKTRCNKADLGPISLNWFEELSSEAPPYNSEPAEESEHKNNNYEPNLFKTPQRKPSYNQLASTPIIFKEQGLTLPLYQSPVKELDKFKLDLGRNVPNSRHKSLRTVKTKMDQADDVSCPLLNSCLSESPVVLQCTHVTPQRDKSVVCGSLFHTPKFVKGRQTPKHISESLGAEVDPDMSWSSSLATPPTLSSTVLIVRNEEASETVFPHDTTANVKSYFSNHDESLKKNDRFIASVTDSENTNQREAASHGFGKTSGNSFKVNSCKDHIGKSMPNVLEDEVYETVVDTSEEDSFSLCFSKCRTKNLQKVRTSKTRKKIFHEANADECEKSKNQVKEKYSFVSEVEPNDTDPLDSNVANQKPFESGSDKISKEVVPSLACEWSQLTLSGLNGAQMEKIPLLHISSCDQNISEKDLLDTENKRKKDFLTSENSLPRISSLPKSEKPLNEETVVNKRDEEQHLESHTDCILAVKQAISGTSPVASSFQGIKKSIFRIRESPKETFNASFSGHMTDPNFKKETEASESGLEIHTVCSQKEDSLCPNLIDNGSWPATTTQNSVALKNAGLISTLKKKTNKFIYAIHDETSYKGKKIPKDQKSELINCSAQFEANAFEAPLTFANADSGLLHSSVKRSCSQNDSEEPTLSLTSSFGTILRKCSRNETCSNNTVISQDLDYKEAKCNKEKLQLFITPEADSLSCLQEGQCENDPKSKKVSDIKEEVLAAACHPVQHSKVEYSDTDFQSQKSLLYDHENASTLILTPTSKDVLSNLVMISRGKESYKMSDKLKGNNYESDVELTKNIPMEKNQDVCALNENYKNVELLPPEKYMRVASPSRKVQFNQNTNLRVIQKNQEETTSISKITVNPDSEELFSDNENNFVFQVANERNNLALGNTKELHETDLTCVNEPIFKNSTMVLYGDTGDKQATQVSIKKDLVYVLAEENKNSVKQHIKMTLGQDLKSDISLNIDKIPEKNNDYMNKWAGLLGPISNHSFGGSFRTASNKEIKLSEHNIKKSKMFFKDIEEQYPTSLACVEIVNTLALDNQKKLSKPQSINTVSAHLQSSVVVSDCKNSHITPQMLFSKQDFNSNHNLTPSQKAEITELSTILEESGSQFEFTQFRKPSYILQKSTFEVPENQMTILKTTSEECRDADLHVIMNAPSIGQVDSSKQFEGTVEIKRKFAGLLKNDCNKSASGYLTDENEVGFRGFYSAHGTKLNVSTEALQKAVKLFSDIENISEETSAEVHPISLSSSKCHDSVVSMFKIENHNDKTVSEKNNKCQLILQNNIEMTTGTFVEEITENYKRNTENEDNKYTAASRNSHNLEFDGSDSSKNDTVCIHKDETDLLFTDQHNICLKLSGQFMKEGNTQIKEDLSDLTFLEVAKAQEACHGNTSNKEQLTATKTEQNIKDFETSDTFFQTASGKNISVAKESFNKIVNFFDQKPEELHNFSLNSELHSDIRKNKMDILSYEETDIVKHKILKESVPVGTGNQLVTFQGQPERDEKIKEPTLLGFHTASGKKVKIAKESLDKVKNLFDEKEQGTSEITSFSHQWAKTLKYREACKDLELACETIEITAAPKCKEMQNSLNNDKNLVSIETVVPPKLLSDNLCRQTENLKTSKSIFLKVKVHENVEKETAKSPATCYTNQSPYSVIENSALAFYTSCSRKTSVSQTSLLEAKKWLREGIFDGQPERINTADYVGNYLYENNSNSTIAENDKNHLSEKQDTYLSNSSMSNSYSYHSDEVYNDSGYLSKNKLDSGIEPVLKNVEDQKNTSFSKVISNVKDANAYPQTVNEDICVEELVTSSSPCKNKNAAIKLSISNSNNFEVGPPAFRIASGKIVCVSHETIKKVKDIFTDSFSKVIKENNENKSKICQTKIMAGCYEALDDSEDILHNSLDNDECSTHSHKVFADIQSEEILQHNQNMSGLEKVSKISPCDVSLETSDICKCSIGKLHKSVSSANTCGIFSTASGKSVQVSDASLQNARQVFSEIEDSTKQVFSKVLFKSNEHSDQLTREENTAIRTPEHLISQKGFSYNVVNSSAFSGFSTASGKQVSILESSLHKVKGVLEEFDLIRTEHSLHYSPTSRQNVSKILPRVDKRNPEHCVNSEMEKTCSKEFKLSNNLNVEGGSSENNHSIKVSPYLSQFQQDKQQLVLGTKVSLVENIHVLGKEQASPKNVKMEIGKTETFSDVPVKTNIEVCSTYSKDSENYFETEAVEIAKAFMEDDELTDSKLPSHATHSLFTCPENEEMVLSNSRIGKRRGEPLILVGEPSIKRNLLNEFDRIIENQEKSLKASKSTPDGTIKDRRLFMHHVSLEPITCVPFRTTKERQEIQNPNFTAPGQEFLSKSHLYEHLTLEKSSSNLAVSGHPFYQVSATRNEKMRHLITTGRPTKVFVPPFKTKSHFHRVEQCVRNINLEENRQKQNIDGHGSDDSKNKINDNEIHQFNKNNSNQAAAVTFTKCEEEPLDLITSLQNARDIQDMRIKKKQRQRVFPQPGSLYLAKTSTLPRISLKAAVGGQVPSACSHKQLYTYGVSKHCIKINSKNAESFQFHTEDYFGKESLWTGKGIQLADGGWLIPSNDGKAGKEEFYRALCDTPGVDPKLISRIWVYNHYRWIIWKLAAMECAFPKEFANRCLSPERVLLQLKYRYDTEIDRSRRSAIKKIMERDDTAAKTLVLCVSDIISLSANISETSSNKTSSADTQKVAIIELTDGWYAVKAQLDPPLLAVLKNGRLTVGQKIILHGAELVGSPDACTPLEAPESLMLKISANSTRPARWYTKLGFFPDPRPFPLPLSSLFSDGGNVGCVDVIIQRAYPIQWMEKTSSGLYIFRNEREEEKEAAKYVEAQQKRLEALFTKIQEEFEEHEENTTKPYLPSRALTRQQVRALQDGAELYEAVKNAADPAYLEGYFSEEQLRALNNHRQMLNDKKQAQIQLEIRKAMESAEQKEQGLSRDVTTVWKLRIVSYSKKEKDSVILSIWRPSSDLYSLLTEGKRYRIYHLATSKSKSKSERANIQLAATKKTQYQQLPVSDEILFQIYQPREPLHFSKFLDPDFQPSCSEVDLIGFVVSVVKKTGLAPFVYLSDECYNLLAIKFWIDLNEDIIKPHMLIAASNLQWRPESKSGLLTLFAGDFSVFSASPKEGHFQETFNKMKNTVENIDILCNEAENKLMHILHANDPKWSTPTKDCTSGPYTAQIIPGTGNKLLMSSPNCEIYYQSPLSLCMAKRKSVSTPVSAQMTSKSCKGEKEIDDQKNCKKRRALDFLSRLPLPPPVSPICTFVSPAAQKAFQPPRSCGTKYETPIKKKELNSPQMTPFKKFNEISLLESNSIADEELALINTQALLSGSTGEKQFISVSESTRTAPTSSEDYLRLKRRCTTSLIKEQESSQASTEECEKNKQDTITTKKYI
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51587 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
Y57 | Phosphorylation | Uniprot | |
K63 | Ubiquitination | Uniprot | |
K68 | Ubiquitination | Uniprot | |
S70 | Phosphorylation | Uniprot | |
Y71 | Phosphorylation | Uniprot | |
S76 | Phosphorylation | Uniprot | |
T77 | Phosphorylation | Uniprot | |
S93 | Phosphorylation | Uniprot | |
K96 | Ubiquitination | Uniprot | |
T167 | Phosphorylation | Uniprot | |
S193 | Phosphorylation | P53350 (PLK1) | Uniprot |
T203 | Phosphorylation | P53350 (PLK1) | Uniprot |
S205 | Phosphorylation | P53350 (PLK1) | Uniprot |
S206 | Phosphorylation | P53350 (PLK1) | Uniprot |
T207 | Phosphorylation | P53350 (PLK1) | Uniprot |
K230 | Ubiquitination | Uniprot | |
S239 | Phosphorylation | P53350 (PLK1) | Uniprot |
K241 | Ubiquitination | Uniprot | |
S253 | Phosphorylation | Uniprot | |
K268 | Methylation | Uniprot | |
K268 | Ubiquitination | Uniprot | |
S273 | Phosphorylation | Uniprot | |
K275 | Methylation | Uniprot | |
K343 | Ubiquitination | Uniprot | |
T363 | Phosphorylation | Uniprot | |
K382 | Ubiquitination | Uniprot | |
S384 | Phosphorylation | Uniprot | |
K426 | Ubiquitination | Uniprot | |
T431 | Phosphorylation | Uniprot | |
S445 | Phosphorylation | Uniprot | |
S451 | Phosphorylation | Uniprot | |
K454 | Acetylation | Uniprot | |
K457 | Ubiquitination | Uniprot | |
K485 | Ubiquitination | Uniprot | |
S492 | Phosphorylation | Uniprot | |
K502 | Ubiquitination | Uniprot | |
S511 | Phosphorylation | Uniprot | |
S519 | Phosphorylation | Uniprot | |
S547 | Phosphorylation | Uniprot | |
S581 | Phosphorylation | Uniprot | |
T582 | Phosphorylation | Uniprot | |
Y592 | Phosphorylation | Uniprot | |
K601 | Ubiquitination | Uniprot | |
K610 | Ubiquitination | Uniprot | |
T675 | Phosphorylation | Uniprot | |
S677 | Phosphorylation | Uniprot | |
T680 | Phosphorylation | Uniprot | |
S683 | Phosphorylation | Uniprot | |
Y688 | Phosphorylation | Uniprot | |
K745 | Ubiquitination | Uniprot | |
S755 | Phosphorylation | Uniprot | |
K757 | Ubiquitination | Uniprot | |
K799 | Ubiquitination | Uniprot | |
S805 | Phosphorylation | Uniprot | |
K811 | Ubiquitination | Uniprot | |
K817 | Ubiquitination | Uniprot | |
K829 | Ubiquitination | Uniprot | |
K838 | Ubiquitination | Uniprot | |
K848 | Ubiquitination | Uniprot | |
K862 | Ubiquitination | Uniprot | |
S879 | Phosphorylation | Uniprot | |
S884 | Phosphorylation | Uniprot | |
K907 | Ubiquitination | Uniprot | |
K945 | Ubiquitination | Uniprot | |
Y949 | Phosphorylation | Uniprot | |
K964 | Ubiquitination | Uniprot | |
S973 | Phosphorylation | Uniprot | |
S976 | Phosphorylation | Uniprot | |
K981 | Ubiquitination | Uniprot | |
K985 | Ubiquitination | Uniprot | |
K992 | Ubiquitination | Uniprot | |
K1094 | Ubiquitination | Uniprot | |
T1104 | Phosphorylation | Uniprot | |
K1108 | Ubiquitination | Uniprot | |
K1132 | Ubiquitination | Uniprot | |
Y1135 | Phosphorylation | Uniprot | |
K1139 | Ubiquitination | Uniprot | |
K1153 | Ubiquitination | Uniprot | |
K1197 | Ubiquitination | Uniprot | |
K1202 | Ubiquitination | Uniprot | |
K1226 | Ubiquitination | Uniprot | |
K1239 | Ubiquitination | Uniprot | |
K1264 | Ubiquitination | Uniprot | |
T1282 | Phosphorylation | Uniprot | |
K1323 | Ubiquitination | Uniprot | |
S1331 | Phosphorylation | Uniprot | |
K1343 | Ubiquitination | Uniprot | |
K1374 | Ubiquitination | Uniprot | |
K1381 | Ubiquitination | Uniprot | |
K1394 | Ubiquitination | Uniprot | |
K1406 | Ubiquitination | Uniprot | |
K1413 | Sumoylation | Uniprot | |
K1413 | Ubiquitination | Uniprot | |
K1419 | Ubiquitination | Uniprot | |
K1434 | Ubiquitination | Uniprot | |
K1440 | Ubiquitination | Uniprot | |
K1453 | Ubiquitination | Uniprot | |
K1474 | Acetylation | Uniprot | |
K1474 | Ubiquitination | Uniprot | |
Y1480 | Phosphorylation | Uniprot | |
K1492 | Ubiquitination | Uniprot | |
K1515 | Ubiquitination | Uniprot | |
K1517 | Ubiquitination | Uniprot | |
S1528 | Phosphorylation | Uniprot | |
K1533 | Acetylation | Uniprot | |
K1536 | Acetylation | Uniprot | |
K1543 | Ubiquitination | Uniprot | |
K1549 | Ubiquitination | Uniprot | |
K1565 | Ubiquitination | Uniprot | |
K1614 | Ubiquitination | Uniprot | |
T1624 | Phosphorylation | Uniprot | |
K1631 | Ubiquitination | Uniprot | |
S1632 | Phosphorylation | Uniprot | |
K1678 | Ubiquitination | Uniprot | |
S1680 | Phosphorylation | Uniprot | |
K1690 | Ubiquitination | Uniprot | |
K1729 | Ubiquitination | Uniprot | |
K1765 | Ubiquitination | Uniprot | |
K1767 | Ubiquitination | Uniprot | |
S1770 | Phosphorylation | Uniprot | |
K1777 | Ubiquitination | Uniprot | |
K1783 | Ubiquitination | Uniprot | |
K1789 | Ubiquitination | Uniprot | |
S1818 | Phosphorylation | Uniprot | |
K1860 | Ubiquitination | Uniprot | |
K1863 | Ubiquitination | Uniprot | |
S1869 | Phosphorylation | Uniprot | |
K1872 | Ubiquitination | Uniprot | |
S1926 | Phosphorylation | Uniprot | |
K1941 | Ubiquitination | Uniprot | |
S1943 | Phosphorylation | Uniprot | |
K1944 | Ubiquitination | Uniprot | |
S1946 | Phosphorylation | Uniprot | |
S1951 | Phosphorylation | Uniprot | |
K1959 | Ubiquitination | Uniprot | |
K1967 | Ubiquitination | Uniprot | |
S1970 | Phosphorylation | Uniprot | |
K1984 | Ubiquitination | Uniprot | |
K2008 | Ubiquitination | Uniprot | |
K2013 | Ubiquitination | Uniprot | |
K2017 | Methylation | Uniprot | |
K2017 | Ubiquitination | Uniprot | |
T2031 | Phosphorylation | Uniprot | |
T2035 | Phosphorylation | Uniprot | |
S2041 | Phosphorylation | Uniprot | |
S2072 | Phosphorylation | Uniprot | |
K2075 | Ubiquitination | Uniprot | |
K2077 | Ubiquitination | Uniprot | |
S2091 | Phosphorylation | Uniprot | |
S2095 | Phosphorylation | Uniprot | |
K2104 | Ubiquitination | Uniprot | |
K2124 | Ubiquitination | Uniprot | |
K2150 | Ubiquitination | Uniprot | |
S2152 | Phosphorylation | Uniprot | |
S2156 | Phosphorylation | Q13535 (ATR) | Uniprot |
K2162 | Ubiquitination | Uniprot | |
S2186 | Phosphorylation | Uniprot | |
K2191 | Ubiquitination | Uniprot | |
K2196 | Ubiquitination | Uniprot | |
K2206 | Ubiquitination | Uniprot | |
K2244 | Ubiquitination | Uniprot | |
K2286 | Ubiquitination | Uniprot | |
K2302 | Ubiquitination | Uniprot | |
K2308 | Ubiquitination | Uniprot | |
K2359 | Ubiquitination | Uniprot | |
Y2363 | Phosphorylation | Uniprot | |
K2404 | Ubiquitination | Uniprot | |
K2411 | Methylation | Uniprot | |
K2411 | Ubiquitination | Uniprot | |
K2413 | Methylation | Uniprot | |
K2448 | Ubiquitination | Uniprot | |
K2459 | Ubiquitination | Uniprot | |
K2472 | Ubiquitination | Uniprot | |
K2498 | Acetylation | Uniprot | |
K2514 | Ubiquitination | Uniprot | |
K2524 | Methylation | Uniprot | |
K2538 | Ubiquitination | Uniprot | |
K2570 | Ubiquitination | Uniprot | |
K2577 | Ubiquitination | Uniprot | |
K2594 | Ubiquitination | Uniprot | |
K2597 | Ubiquitination | Uniprot | |
K2640 | Ubiquitination | Uniprot | |
K2657 | Ubiquitination | Uniprot | |
K2741 | Ubiquitination | Uniprot | |
K2882 | Ubiquitination | Uniprot | |
Y2905 | Phosphorylation | Uniprot | |
K2950 | Methylation | Uniprot | |
S2954 | Phosphorylation | Uniprot | |
K2958 | Ubiquitination | Uniprot | |
T2967 | Phosphorylation | Uniprot | |
T2968 | Phosphorylation | Uniprot | |
K3031 | Ubiquitination | Uniprot | |
K3179 | Ubiquitination | Uniprot | |
T3193 | Phosphorylation | Uniprot | |
K3196 | Ubiquitination | Uniprot | |
T3199 | Phosphorylation | Uniprot | |
T3211 | Phosphorylation | Uniprot | |
S3231 | Phosphorylation | Uniprot | |
K3238 | Ubiquitination | Uniprot | |
S3239 | Phosphorylation | Uniprot | |
T3242 | Phosphorylation | Uniprot | |
S3250 | Phosphorylation | Uniprot | |
S3284 | Phosphorylation | Uniprot | |
S3291 | Phosphorylation | P06493 (CDK1) | Uniprot |
T3306 | Phosphorylation | Uniprot | |
S3319 | Phosphorylation | Uniprot | |
T3323 | Phosphorylation | Uniprot | |
T3387 | Phosphorylation | PR:O14757 (hCHEK1) , PR:O96017 (hCHEK2) | Uniprot |
S3397 | Phosphorylation | Uniprot |
Research Backgrounds
Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination.
Phosphorylated by ATM upon irradiation-induced DNA damage. Phosphorylation by CHEK1 and CHEK2 regulates interaction with RAD51. Phosphorylation at Ser-3291 by CDK1 and CDK2 is low in S phase when recombination is active, but increases as cells progress towards mitosis; this phosphorylation prevents homologous recombination-dependent repair during S phase and G2 by inhibiting RAD51 binding.
Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation.
Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
Monomer and dimer. Interacts with RAD51; regulates RAD51 recruitment and function at sites of DNA repair. Interacts with WDR16, USP11, DMC1, ROCK2 and NPM1. Interacts with SEM1; the interaction masks a nuclear export signal in BRCA2. Interacts with both nonubiquitinated and monoubiquitinated FANCD2; this complex also includes XRCC3 and phosphorylated FANCG. Part of a BRCA complex containing BRCA1, BRCA2 and PALB2. Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with BRCA1 only in the presence of PALB2 which serves as the bridging protein. Interacts with POLH; the interaction is direct. Interacts with the TREX-2 complex subunits PCID2 and SEM1.
Research Fields
· Genetic Information Processing > Replication and repair > Homologous recombination.
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)
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