Phospho-TNNI3 (Ser23+Ser24) Antibody

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Product:	Phospho-TNNI3 (Ser23+Ser24) Antibody
Catalog:	AF7307
Description:	Rabbit polyclonal antibody to Phospho-TNNI3 (Ser23+Ser24)
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	26kDa; 24kD(Calculated).
Uniprot:	P48787
RRID:	AB_2843747

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-TNNI3 (Ser23+Ser24) Antibody detects endogenous levels of TNNI3 only when phosphorylated at Ser23+Ser24.

RRID:

AB_2843747
Cite Format: Affinity Biosciences Cat# AF7307, RRID:AB_2843747.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

cardiac muscle; Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); Cardiomyopathy, familial hypertrophic, 7, included; CMD1FF; CMD2A; CMH7; cTnI; Familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; Tn1; Tni; TNN I3; TNNC 1; TNNC1; TNNI3; TNNI3_HUMAN; Troponin I; Troponin I cardiac; Troponin I cardiac muscle; Troponin I cardiac muscle isoform; Troponin I type 3 cardiac; troponin I, cardiac 3; TroponinI; Ttroponin I type 3 (cardiac);

Immunogens

Immunogen:

A synthesized peptide derived from mouse TNNI3 around the phosphorylation site of Ser23+Ser24.

Uniprot:

P48787(TNNI3_MOUSE) >>Visit HPA database.

Sequence:

P48787 TNNI3_MOUSE:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADESSDAAGEPQPAPAPVRRRSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQEMEREAEERRGEKGRVLRTRCQPLELDGLGFEELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLTQKIYDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKEDIEKENREVGDWRKNIDALSGMEGRKKKFEG

PTMs - P48787 As Substrate

P48787

Site	PTM Type	Enzyme	Source
S5	Phosphorylation		Uniprot
S6	Phosphorylation		Uniprot
S23	Phosphorylation	P68404-2 (Prkcb) , P16054 (Prkce) , P05132 (Prkaca) , P70336 (Rock2) , P20444 (Prkca) , P18653 (Rps6ka1)	Uniprot
S24	Phosphorylation	P68404-2 (Prkcb) , P18653 (Rps6ka1) , P05132 (Prkaca) , P20444 (Prkca) , P70336 (Rock2) , P16054 (Prkce)	Uniprot
Y27	Phosphorylation		Uniprot
K37	Ubiquitination		Uniprot
S43	Phosphorylation	P68404-2 (Prkcb) , P20444 (Prkca)	Uniprot
S45	Phosphorylation	P20444 (Prkca) , P68404-2 (Prkcb)	Uniprot
K51	Ubiquitination		Uniprot
K59	Ubiquitination		Uniprot
C81	S-Nitrosylation		Uniprot
C98	S-Nitrosylation		Uniprot
K107	Ubiquitination		Uniprot
K118	Ubiquitination		Uniprot
K121	Ubiquitination		Uniprot
K132	Ubiquitination		Uniprot
Y134	Phosphorylation		Uniprot
T144	Phosphorylation	P68404-2 (Prkcb) , P70336 (Rock2) , P20444 (Prkca)	Uniprot
S151	Phosphorylation		Uniprot
K165	Ubiquitination		Uniprot
S167	Phosphorylation		Uniprot
K175	Ubiquitination		Uniprot
K184	Ubiquitination		Uniprot
K194	Acetylation		Uniprot
K194	Ubiquitination		Uniprot
S200	Phosphorylation		Uniprot

Research Backgrounds

P48787_TNNI3_MOUSE

Function:

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

PTMs:

Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T) (By similarity). Phosphorylated at Ser-43 and Ser-45 by PRKCE; phosphorylation increases myocardium contractile dysfunction.

Subunit Structure:

Interacts with TRIM63 (By similarity). Binds to actin and tropomyosin. Interacts with STK4/MST1 (By similarity).

Family&Domains:

Belongs to the troponin I family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-TNNI3 (Ser23+Ser24) Antibody - #AF7307