Phospho-MCM4 (Ser88) Antibody - #AF7294

(3)  
Product: Phospho-MCM4 (Ser88) Antibody
Catalog: AF7294
Description: Rabbit polyclonal antibody to Phospho-MCM4 (Ser88)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 97kDa; 97kD(Calculated).
Uniprot: P33991
RRID: AB_2843734

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(80%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-MCM4 (Ser88) Antibody detects endogenous levels of MCM4 only when phosphorylated at Ser88.
RRID:
AB_2843734
Cite Format: Affinity Biosciences Cat# AF7294, RRID:AB_2843734.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

19G; AI325074; AU045576; CDC 21; CDC 54; CDC21; CDC21 homolog; CDC21, S. pombe, homolog of; CDC54; Cell division cycle 21, S. pombe, homolog of; DNA replication licensing factor MCM 4; DNA replication licensing factor MCM4; hCdc 21; hCdc21; Homolog of S. pombe cell devision cycle 21; KIAA4003; mcdc21; MCM 4; MCM 4 minichromosome maintenance deficient 4; mcm4; MCM4 minichromosome maintenance deficient 4; MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae); MCM4_HUMAN; Mcmd4; MGC33310; Minichromosome maintenance 4; Minichromosome maintenance complex component 4; Minichromosome maintenance deficient (S. cerevisiae) 4; Minichromosome maintenance deficient 4; Minichromosome maintenance deficient 4 homolog (S. cerevisiae); Minichromosome maintenance, S. cerevisiae, homolog of, 4; mKIAA4003; NKCD; NKGCD; P1 CDC21; P1-CDC21; P1CDC21;

Immunogens

Immunogen:
Uniprot:

P33991(MCM4_HUMAN) >>Visit HPA database.

Gene(ID):
MCM4(4173)
Sequence:
MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGVDLQSPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPLAKEEENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSFDKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDIDQLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQMIKLQESPEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLLLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKTVRLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
80
Zebrafish
78
Bovine
0
Sheep
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P33991 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S3 Phosphorylation P24941 (CDK2)
S6 Phosphorylation
T7 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
S9 Phosphorylation
R10 Methylation
R11 Methylation
S13 Phosphorylation
R14 Methylation
R15 Methylation
R17 Methylation
T19 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
T23 Phosphorylation
S26 Phosphorylation
S31 Phosphorylation
S32 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
S34 Phosphorylation
T53 Phosphorylation P24941 (CDK2)
S54 Phosphorylation P24941 (CDK2)
S61 Phosphorylation
S71 Phosphorylation
S78 Phosphorylation
S87 Phosphorylation
S88 Phosphorylation P06493 (CDK1)
T91 Phosphorylation
Y92 Phosphorylation
T94 Phosphorylation
T102 Phosphorylation
S105 Phosphorylation
R108 Methylation
T110 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
S120 Phosphorylation
K123 Acetylation
K123 Ubiquitination
S131 Phosphorylation
S142 Phosphorylation
S145 Phosphorylation
K163 Ubiquitination
K179 Ubiquitination
Y193 Phosphorylation
K216 Methylation
K216 Ubiquitination
S217 Phosphorylation
K220 Acetylation
K220 Ubiquitination
Y223 Phosphorylation
T236 Phosphorylation
K267 Ubiquitination
S326 Phosphorylation
K349 Ubiquitination
S357 Phosphorylation
K381 Ubiquitination
R387 Methylation
Y394 Phosphorylation
S406 Phosphorylation
K409 Ubiquitination
K413 Methylation
K413 Ubiquitination
K427 Ubiquitination
K439 Acetylation
K439 Sumoylation
K439 Ubiquitination
K444 Ubiquitination
K450 Acetylation
K450 Ubiquitination
S453 Phosphorylation
K455 Ubiquitination
Y459 Phosphorylation
S464 Phosphorylation
K477 Sumoylation
K477 Ubiquitination
K478 Ubiquitination
T488 Phosphorylation
K490 Ubiquitination
Y522 Phosphorylation
Y524 Phosphorylation
K536 Ubiquitination
K549 Ubiquitination
K578 Ubiquitination
K600 Ubiquitination
K627 Acetylation
K627 Ubiquitination
K628 Ubiquitination
S707 Phosphorylation
Y714 Phosphorylation
R724 Methylation
S736 Phosphorylation
K746 Ubiquitination
K752 Ubiquitination
K762 Ubiquitination
K770 Ubiquitination
S784 Phosphorylation
T788 Phosphorylation
K798 Ubiquitination
K806 Methylation
K814 Ubiquitination
K819 Acetylation
K819 Ubiquitination
R828 Methylation
K837 Ubiquitination
K858 Acetylation
K858 Ubiquitination

Research Backgrounds

Function:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

PTMs:

Sumoylated; SUMO2 modified in response to stress caused by inhibition of proteasome activity (in vitro).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with MCMBP.

Family&Domains:

Belongs to the MCM family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.