Phospho-Phospholamban (Ser16/Thr17) Antibody

IHC
IF/ICC
pElisa
Cites

1/4

AF7278 at 1/100 staining rat heart tissue sections by IHC-P.

AF7278 staining Hela cells(4h of LPS treatment) by IF/ICC.

peptide-ELISA analysis of AF7278.

FIGURE 4 | Upregulation of miR-146a decreased the abnormal expression of cardiac PLN and ANK2 caused by SNT.

Product:	Phospho-Phospholamban (Ser16/Thr17) Antibody
Catalog:	AF7278
Description:	Rabbit polyclonal antibody to Phospho-Phospholamban (Ser16/Thr17)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	24kDa; 6kD(Calculated).
Uniprot:	P26678
RRID:	AB_2843718

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-Phospholamban (Ser16/Thr17) Antibody detects endogenous levels of Phospholamban only when phosphorylated at Ser16/Thr17.

RRID:

AB_2843718
Cite Format: Affinity Biosciences Cat# AF7278, RRID:AB_2843718.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cardiac phospholamban; CMD1P; CMH18; PLB; Pln; PPLA_HUMAN;

Immunogens

Immunogen:

Uniprot:

P26678(PPLA_HUMAN) >>Visit HPA database.

Gene(ID):

PLN(5350)

Expression:

P26678 PPLA_HUMAN:

Heart muscle (at protein level).

Sequence:

P26678 PPLA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEKVQYLTRSAIRRASTIEMPQQARQKLQNLFINFCLILICLLLICIIVMLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P26678 As Substrate

P26678

Site	PTM Type	Enzyme	Source
S16	Phosphorylation	Q09013 (DMPK) , P17612 (PRKACA) , P31749 (AKT1)	Uniprot
T17	Phosphorylation	Q13557 (CAMK2D) , Q9UQM7 (CAMK2A) , Q13554 (CAMK2B)	Uniprot

Research Backgrounds

P26678_PPLA_HUMAN

Function:

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.

PTMs:

Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.

Palmitoylated by ZDHHC16, promoting formation of the homopentamer.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Sarcoplasmic reticulum membrane>Single-pass membrane protein. Mitochondrion membrane>Single-pass membrane protein. Membrane>Single-pass membrane protein.
Note: Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).

Tissue Specificity:

Heart muscle (at protein level).

Subunit Structure:

Homopentamer. Interacts with HAX1 and ATP2A2.

Family&Domains:

Belongs to the phospholamban family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

References

1). Downregulation of miR-146a Contributes to Cardiac Dysfunction Induced by the Tyrosine Kinase Inhibitor Sunitinib. Frontiers in Pharmacology (PubMed: 31507414) [IF=5.6]

Application: WB Species: mouse Sample: myocardial

FIGURE 4 | Upregulation of miR-146a decreased the abnormal expression of cardiac PLN and ANK2 caused by SNT. (A) Pln and Ank2 mRNA levels in myocardium of mice with different treatments. Values are normalized to the level of GAPDH. (B) Representative Western Blot bands for PLN and p-PLN protein expression and corresponding summary data. Band densitometry was normalized to Tubulin.

2). A-kinase Anchoring Protein 5 Anchors Protein Kinase A to Mediate PLN/SERCA to Reduce Cardiomyocyte Apoptosis Induced by Hypoxia and Reoxygenation. Biochemistry and Cell Biology (PubMed: 35041539) [IF=2.9]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-Phospholamban (Ser16/Thr17) Antibody - #AF7278