Phospho-MCM3 (Ser160) Antibody - #AF7275

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Product: Phospho-MCM3 (Ser160) Antibody
Catalog: AF7275
Description: Rabbit polyclonal antibody to Phospho-MCM3 (Ser160)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 91kDa; 91kD(Calculated).
Uniprot: P25205
RRID: AB_2843715

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Bovine(90%), Horse(100%), Sheep(90%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Phospho-MCM3 (Ser160) Antibody detects endogenous levels of MCM3 only when phosphorylated at Ser160.
RRID:
AB_2843715
Cite Format: Affinity Biosciences Cat# AF7275, RRID:AB_2843715.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cervical cancer proto oncogene 5; DNA polymerase alpha holoenzyme associated P1; DNA polymerase alpha holoenzyme associated protein P1; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor mcm3; HCC 5; HCC5; hRlf beta subunit; Human cervical cancer proto oncogene 5; MCM 3; mcm3; MCM3 minichromosome maintenance deficient 3; MCM3_HUMAN; MGC1157; Minichromosome maintenance complex component 3; Minichromosome maintenance deficient 3; Minichromosome maintenance protein 3; P1 h; P1 MCM3; P1 Protein; P1-MCM3; P1.h; p102; P102 protein; Replication licensing factor beta subunit; RLF beta subunit; RLF subunit beta; RLFB;

Immunogens

Immunogen:
Uniprot:

P25205(MCM3_HUMAN) >>Visit HPA database.

Gene(ID):
MCM3(4172)
Sequence:
MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
90
Bovine
90
Sheep
90
Xenopus
80
Chicken
80
Zebrafish
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25205 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T4 Phosphorylation
Y19 Phosphorylation
Y32 Phosphorylation
S34 Phosphorylation
K35 Ubiquitination
Y45 Phosphorylation
R55 Methylation
K81 Ubiquitination
S112 Phosphorylation P06493 (CDK1)
K140 Ubiquitination
S144 Phosphorylation
C148 S-Nitrosylation
K152 Acetylation
K152 Ubiquitination
T154 Phosphorylation
Y159 Phosphorylation
S160 Phosphorylation P53355 (DAPK1) , O14757 (CHEK1)
T163 Phosphorylation
T164 Phosphorylation
S170 Phosphorylation
S171 Phosphorylation
T176 Phosphorylation
K177 Ubiquitination
T198 Phosphorylation
K207 Ubiquitination
K228 Ubiquitination
K230 Ubiquitination
R234 Methylation
T240 Phosphorylation
K248 Sumoylation
K248 Ubiquitination
T252 Phosphorylation
T255 Phosphorylation
T258 Phosphorylation
C263 S-Nitrosylation
K266 Sumoylation
K266 Ubiquitination
K270 Ubiquitination
S275 Phosphorylation
K283 Ubiquitination
S292 Phosphorylation
K293 Ubiquitination
K301 Ubiquitination
Y312 Phosphorylation
K314 Ubiquitination
K315 Ubiquitination
K351 Ubiquitination
R371 Methylation
T383 Phosphorylation
T384 Phosphorylation
K413 Ubiquitination
R419 Methylation
K435 Ubiquitination
K463 Ubiquitination
S474 Phosphorylation
S535 Phosphorylation Q13535 (ATR) , Q13315 (ATM)
Y545 Phosphorylation
T555 Phosphorylation
K556 Acetylation
K556 Ubiquitination
K559 Acetylation
K561 Ubiquitination
K570 Ubiquitination
K579 Ubiquitination
S599 Phosphorylation
S611 Phosphorylation
T617 Phosphorylation
K635 Ubiquitination
Y651 Phosphorylation
K655 Ubiquitination
S668 Phosphorylation
S672 Phosphorylation
T674 Phosphorylation
S681 Phosphorylation
K688 Ubiquitination
S704 Phosphorylation
Y705 Phosphorylation
Y708 Phosphorylation
S711 Phosphorylation
T713 Phosphorylation
T722 Phosphorylation P24941 (CDK2)
T725 Phosphorylation
S728 Phosphorylation Q13315 (ATM)
K732 Sumoylation
K732 Ubiquitination
S734 Phosphorylation
K736 Sumoylation
K736 Ubiquitination
S740 Phosphorylation
S742 Phosphorylation
R743 Methylation
K748 Ubiquitination

Research Backgrounds

Function:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

PTMs:

Acetylated by MCM3AP.

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Associated with the replication-specific DNA polymerase alpha. Interacts with MCMBP. Interacts with ANKRD17. Interacts with MCM3AP isoform MCM3AP; this interaction leads to MCM3 acetylation.

Family&Domains:

Belongs to the MCM family.

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