Phospho-BCL-3 (Ser41) Antibody | Affinity Biosciences

IHC
pElisa

1/2

AF7256 at 1/100 staining human lung cancer tissue sections by IHC-P.

Product:	Phospho-BCL-3 (Ser41) Antibody
Catalog:	AF7256
Description:	Rabbit polyclonal antibody to Phospho-BCL-3 (Ser41)
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Bovine, Rabbit, Dog
Mol.Wt.:	48kDa; 48kD(Calculated).
Uniprot:	P20749
RRID:	AB_2843696

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(100%), Rabbit(100%), Dog(90%)

Clonality:

Polyclonal

Specificity:

Phospho-BCL-3 (Ser41) Antibody detects endogenous levels of BCL-3 only when phosphorylated at Ser41.

RRID:

AB_2843696
Cite Format: Affinity Biosciences Cat# AF7256, RRID:AB_2843696.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

B cell CLL/lymphoma 3; B cell leukemia/lymphoma 3; B cell lymphoma 3 encoded protein; B-cell lymphoma 3 protein; Bcl 3; BCL 4; BCL-3; Bcl3; BCL3_HUMAN; BCL4; Chronic lymphatic leukemia protein; D19S37; Protein Bcl 3; Proto-oncogene BCL3;

Immunogens

Immunogen:

Uniprot:

P20749(BCL3_HUMAN) >>Visit HPA database.

Gene(ID):

BCL3(602)

Sequence:

P20749 BCL3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPRCPAGAMDEGPVDLRTRPKAAGLPGAALPLRKRPLRAPSPEPAAPRGAAGLVVPLDPLRGGCDLPAVPGPPHGLARPEALYYPGALLPLYPTRAMGSPFPLVNLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Bovine

100

Dog

Sheep

Pig

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20749 As Substrate

P20749

Site	PTM Type	Enzyme	Source
K21	Ubiquitination		Uniprot
K34	Ubiquitination		Uniprot
S41	Phosphorylation		Uniprot
Y83	Phosphorylation		Uniprot
Y84	Phosphorylation		Uniprot
S122	Phosphorylation		Uniprot
K338	Methylation		Uniprot
S374	Phosphorylation		Uniprot
S386	Phosphorylation		Uniprot
S387	Phosphorylation		Uniprot
S402	Phosphorylation	P49840 (GSK3A)	Uniprot
S406	Phosphorylation	P49840 (GSK3A)	Uniprot
S448	Phosphorylation		Uniprot
S454	Phosphorylation		Uniprot

Research Backgrounds

P20749_BCL3_HUMAN

Function:

Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity).

PTMs:

Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation (By similarity).

Activated by phosphorylation.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Perinuclear region.
Note: Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.

Subunit Structure:

Component of a complex consisting of the NF-kappa-B p52-p52 homodimer and BCL3. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Interacts with N4BP2, COPS5 and PIR. Interacts with CYLD (By similarity).

Research Fields

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-BCL-3 (Ser41) Antibody - #AF7256