Phospho-GABA A Receptor beta 1 (Ser409) Antibody

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Product:	Phospho-GABA A Receptor beta 1 (Ser409) Antibody
Catalog:	AF7251
Description:	Rabbit polyclonal antibody to Phospho-GABA A Receptor beta 1 (Ser409)
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	55kDa; 54kD(Calculated).
Uniprot:	P18505
RRID:	AB_2843691

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(86%), Bovine(86%), Horse(100%), Sheep(86%), Rabbit(100%), Dog(86%)

Clonality:

Polyclonal

Specificity:

Phospho-GABA A Receptor beta 1 (Ser409) Antibody detects endogenous levels of GABA A Receptor beta 1 only when phosphorylated at Ser409.

RRID:

AB_2843691
Cite Format: Affinity Biosciences Cat# AF7251, RRID:AB_2843691.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AW061132; B230208N19Rik; GABA(A) receptor beta 1; GABA(A) receptor subunit beta-1; GABA-A receptor, beta-1 polypeptide; Gabrb-1; GABRB1; Gamma aminobutyric acid (GABA) A receptor beta 1; Gamma Aminobutyric Acid A Receptor Beta 1; Gamma Aminobutyric Acid Receptor , beta-1; Gamma-aminobutyric acid (GABA) A receptor, subunit beta 1; Gamma-aminobutyric acid receptor subunit beta-1; GARB1; GBRB1_HUMAN;

Immunogens

Immunogen:

Uniprot:

P18505(GBRB1_HUMAN) >>Visit HPA database.

Gene(ID):

GABRB1(2560)

Sequence:

P18505 GBRB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWTVQNRESLGLLSFPVMITMVCCAHSTNEPSNMSYVKETVDRLLKGYDIRLRPDFGGPPVDVGMRIDVASIDMVSEVNMDYTLTMYFQQSWKDKRLSYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWNGGEGAVTGVNKIELPQFSIVDYKMVSKKVEFTTGAYPRLSLSFRLKRNIGYFILQTYMPSTLITILSWVSFWINYDASAARVALGITTVLTMTTISTHLRETLPKIPYVKAIDIYLMGCFVFVFLALLEYAFVNYIFFGKGPQKKGASKQDQSANEKNKLEMNKVQVDAHGNILLSTLEIRNETSGSEVLTSVSDPKATMYSYDSASIQYRKPLSSREAYGRALDRHGVPSKGRIRRRASQLKVKIPDLTDVNSIDKWSRMFFPITFSLFNVVYWLYYVH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

100

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18505 As Substrate

P18505

Site	PTM Type	Enzyme	Source
K46	Ubiquitination		Uniprot
T85	Phosphorylation		Uniprot
K127	Ubiquitination		Uniprot
K128	Ubiquitination		Uniprot
K137	Ubiquitination		Uniprot
Y309	Phosphorylation		Uniprot
Y324	Phosphorylation		Uniprot
S409	Phosphorylation		Uniprot
S434	Phosphorylation	P17612 (PRKACA)	Uniprot
S448	Phosphorylation		Uniprot

Research Backgrounds

P18505_GBRB1_HUMAN

Function:

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.

Subcellular Location:

Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.

Subunit Structure:

Binds UBQLN1 (By similarity). Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains. Interacts with KCTD8, KCTD12 and KCTD16; this interaction determines the pharmacology and kinetics of the receptor response, the KCTD proteins markedly accelerating the GABA-B response, although to different extents (By similarity).

Family&Domains:

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB1 sub-subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-GABA A Receptor beta 1 (Ser409) Antibody - #AF7251