Phospho-Synaptotagmin 1/2 (Ser309) Antibody - #AF3223
| Product: | Phospho-Synaptotagmin 1/2 (Ser309) Antibody |
| Catalog: | AF3223 |
| Description: | Rabbit polyclonal antibody to Phospho-Synaptotagmin 1/2 (Ser309) |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 47kDa; 48kD,47kD(Calculated). |
| Uniprot: | P21579 | Q8N9I0 |
| RRID: | AB_2834651 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3223, RRID:AB_2834651.
Fold/Unfold
DKFZp781D2042; FLJ42519; p65; SVP65; Synaptotagmin 2; Synaptotagmin I; Synaptotagmin II; Synaptotagmin-1; SYT; Syt1; SYT1_HUMAN; Syt2; SytI; Synaptotagmin 2; Synaptotagmin II; Synaptotagmin-2; Synaptotagmin2; SynaptotagminII; SYT 2; Syt II; Syt2; SYT2_HUMAN; SytII;
Immunogens
Expressed in melanocytes (PubMed:23999003).
Q8N9I0 SYT2_HUMAN:Expressed in melanocytes (PubMed:23999003).
- P21579 SYT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKEKFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK
- Q8N9I0 SYT2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRNIFKRNQEPIVAPATTTATMPIGPVDNSTESGGAGESQEDMFAKLKEKLFNEINKIPLPPWALIAIAVVAGLLLLTCCFCICKKCCCKKKKNKKEKGKGMKNAMNMKDMKGGQDDDDAETGLTEGEGEGEEEKEPENLGKLQFSLDYDFQANQLTVGVLQAAELPALDMGGTSDPYVKVFLLPDKKKKYETKVHRKTLNPAFNETFTFKVPYQELGGKTLVMAIYDFDRFSKHDIIGEVKVPMNTVDLGQPIEEWRDLQGGEKEEPEKLGDICTSLRYVPTAGKLTVCILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTVKKKTLNPYFNESFSFEIPFEQIQKVQVVVTVLDYDKLGKNEAIGKIFVGSNATGTELRHWSDMLANPRRPIAQWHSLKPEEEVDALLGKNK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P21579/Q8N9I0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S24 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| T126 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| T212 | Phosphorylation | Uniprot | |
| Y217 | Phosphorylation | Uniprot | |
| Y230 | Phosphorylation | Uniprot | |
| K237 | Acetylation | Uniprot | |
| K302 | Acetylation | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K46 | Acetylation | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| T122 | Phosphorylation | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| Y178 | Phosphorylation | Uniprot | |
| T199 | Phosphorylation | Q9H4A3 (WNK1) | Uniprot |
| K299 | Acetylation | Uniprot | |
| Y309 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| T381 | Phosphorylation | Uniprot | |
| T383 | Phosphorylation | Q9H4A3 (WNK1) | Uniprot |
Research Backgrounds
Calcium sensor that participates in triggering neurotransmitter release at the synapse (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes.
Glycosylated.
Cytoplasmic vesicle>Secretory vesicle membrane>Single-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Single-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle>Chromaffin granule membrane>Single-pass membrane protein. Cytoplasm.
Expressed in melanocytes.
Homotetramer (Probable). Interacts with SCAMP5. Interacts with STON2. Forms a complex with SV2B, syntaxin 1 and SNAP25 (By similarity). Interacts with SV2A, SV2B and SV2C (By similarity). Interacts with RIMS1 (By similarity). Interacts with PRRT2 (By similarity). Interacts with DNAJC5 in a phosphorylation-dependent manner (By similarity). Interacts (via N-terminus) with RAB3A (By similarity). Interacts with SYT12 (By similarity). Interacts with calmodulin (By similarity).
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with SV2A and probably with STN2.
Belongs to the synaptotagmin family.
Exhibits calcium-dependent phospholipid and inositol polyphosphate binding properties (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). Plays a role in dendrite formation by melanocytes.
Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane>Single-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle>Chromaffin granule membrane>Single-pass membrane protein.
Expressed in melanocytes.
Homotetramer (Probable). Interacts with STON2. Interacts with SCAMP5. Interacts with PRRT2 (By similarity).
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2. The second C2 domain mediates phospholipid and inositol polyphosphate binding in a calcium-independent manner.
Belongs to the synaptotagmin family.
Research Fields
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
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