Phospho-Synapsin I (Ser427) Antibody

WB
IHC
pElisa
Cites

1/6

Western blot analysis of extracts from Mouse brain, using Phospho-Synapsin I (Ser427) Antibody.

Western blot analysis of extracts from various samples, using Phospho-Synapsin I (Ser427) Antibody.

AF7245 at 1/100 staining rat brain tissue sections by IHC-P.

peptide-ELISA analysis of AF7245.

FIGURE 6 Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells.

FIGURE 6 | Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells.

Product:	Phospho-Synapsin I (Ser427) Antibody
Catalog:	AF7245
Description:	Rabbit polyclonal antibody to Phospho-Synapsin I (Ser427)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	77kDa; 74kD(Calculated).
Uniprot:	P17600
RRID:	AB_2843685

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-Synapsin I (Ser427) Antibody detects endogenous levels of Synapsin I only when phosphorylated at Ser427.

RRID:

AB_2843685
Cite Format: Affinity Biosciences Cat# AF7245, RRID:AB_2843685.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Brain protein 4.1; SYN 1; SYN 1a; SYN 1b; SYN I; SYN1; SYN1_HUMAN; SYN1a; SYN1b; Synapsin 1; Synapsin I; Synapsin-1; Synapsin1; SynapsinI; SYNI;

Immunogens

Immunogen:

Uniprot:

P17600(SYN1_HUMAN) >>Visit HPA database.

Gene(ID):

SYN1(6853)

Sequence:

P17600 SYN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPGAHSPGATPGPGTATAERSSGVAPAASPAAPSPGSSGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSGGAGRGGAASRVLLVIDEPHTDWAKYFKGKKIHGEIDIKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGIPSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKVKVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMSDRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMAQALPRQRQRDASPGRGSHGQTPSPGALPLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQQHLSGLGPPAGSPLPQRLPSPTSAPQQPASQAAPPTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGPPQATRQTSVSGPAPPKASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPVPRTGPPTTQQPRPSGPGPAGRPKPQLAQKPSQDVPPPATAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFASLFSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17600 As Substrate

P17600

Site	PTM Type	Enzyme	Source
S9	Phosphorylation	Q14012 (CAMK1) , Q13131 (PRKAA1)	Uniprot
S11	Phosphorylation		Uniprot
S39	Phosphorylation		Uniprot
S62	Phosphorylation	P27361 (MAPK3)	Uniprot
S67	Phosphorylation	P27361 (MAPK3)	Uniprot
S293	Phosphorylation		Uniprot
Y301	Phosphorylation		Uniprot
S332	Phosphorylation		Uniprot
S427	Phosphorylation		Uniprot
S432	Phosphorylation		Uniprot
T436	Phosphorylation		Uniprot
S438	Phosphorylation	Q8TAS1 (UHMK1)	Uniprot
T448	Phosphorylation		Uniprot
S449	Phosphorylation		Uniprot
S551	Phosphorylation	P27361 (MAPK3) , Q00535 (CDK5)	Uniprot
S553	Phosphorylation	Q00535 (CDK5)	Uniprot
T567	Phosphorylation		Uniprot
S568	Phosphorylation		Uniprot
S605	Phosphorylation		Uniprot
T613	Phosphorylation		Uniprot
S698	Phosphorylation		Uniprot

Research Backgrounds

P17600_SYN1_HUMAN

Function:

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.

PTMs:

Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal.

Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.

Subcellular Location:

Cell junction>Synapse. Golgi apparatus.

Subunit Structure:

Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ib interacts with PRNP (By similarity).

Family&Domains:

The A region binds phospholipids with a preference for negatively charged species.

Belongs to the synapsin family.

References

1). CHIP Decline Is Associated With Isoflurane-Induced Neurodegeneration in Aged Mice. Frontiers in Neuroscience, 2022 (PubMed: 35368262) [IF=4.3]

Application: WB Species: Mice Sample: N2a cells

FIGURE 6 Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells. (A) CHIP knock-down protein was designed by siRNA in N2a cells. Approximately 5 μl Stub1-siRNA was able to achieve effective knock-down CHIP expression, and consequently the expression level of synapsin I S9 was decreased with no change in synaptic protein SNAP25 and PSD95 expression. (B) Statistical bar chart to show that CHIP, synapsin I, SNAP25, and PSD95 expression were present (n = 6, **p < 0.01, ***p < 0.001). (C) The dose of 5 μl Stub1-siRNA showed a decrease in the expression levels of ubiquitin and synapsin I and the phosphorylation levels of synapsin I S9, S427, and S605. (D) Statistical bar chart to show that ubiquitin, synapsin I, synapsin I S9, S427, and S605 expression were present (n = 6, *p < 0.05).

Application: WB Species: mouse Sample: N2a cells

FIGURE 6 | Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells. (B) Statistical bar chart to show that CHIP, synapsin I, SNAP25, and PSD95 expression were present (n = 6, **p < 0.01, ***p < 0.001).

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Phospho-Synapsin I (Ser427) Antibody - #AF7245