Phospho-JunB (Thr255) Antibody - #AF7241

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Product: Phospho-JunB (Thr255) Antibody
Catalog: AF7241
Description: Rabbit polyclonal antibody to Phospho-JunB (Thr255)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 38kDa; 36kD(Calculated).
Uniprot: P17275
RRID: AB_2843681

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-JunB (Thr255) Antibody detects endogenous levels of JunB only when phosphorylated at Thr255.
RRID:
AB_2843681
Cite Format: Affinity Biosciences Cat# AF7241, RRID:AB_2843681.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activator protein 1; AP 1; AP1; Jun B; Jun B proto oncogene; Jun B protooncogene; Junb; JunB proto oncogene; JunB protoncogene 9; JunB protooncogene; JUNB_HUMAN; Transcription factor jun B; Transcription factor jun-B; Transcription factor junB;

Immunogens

Immunogen:
Uniprot:

P17275(JUNB_HUMAN) >>Visit HPA database.

Gene(ID):
JUNB(3726)
Sequence:
MCTKMEQPFYHDDSYTATGYGRAPGGLSLHDYKLLKPSLAVNLADPYRSLKAPGARGPGPEGGGGGSYFSGQGSDTGASLKLASSELERLIVPNSNGVITTTPTPPGQYFYPRGGGSGGGAGGAGGGVTEEQEGFADGFVKALDDLHKMNHVTPPNVSLGATGGPPAGPGGVYAGPEPPPVYTNLSSYSPASASSGGAGAAVGTGSSYPTTTISYLPHAPPFAGGHPAQLGLGRGASTFKEEPQTVPEARSRDATPPVSPINMEDQERIKVERKRLRNRLAATKCRKRKLERIARLEDKVKTLKAENAGLSSTAGLLREQVAQLKQKVMTHVSNGCQLLLGVKGHAF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17275 As Substrate

Site PTM Type Enzyme
K4 Sumoylation
K33 Sumoylation
K36 Acetylation
K36 Sumoylation
K36 Ubiquitination
Y47 Phosphorylation
S49 Phosphorylation
K51 Sumoylation
Y68 Phosphorylation
S79 Phosphorylation Q16539 (MAPK14)
T102 Phosphorylation Q16539 (MAPK14) , P45983 (MAPK8)
T104 Phosphorylation Q16539 (MAPK14) , P45983 (MAPK8)
Y109 Phosphorylation
Y111 Phosphorylation
S117 Phosphorylation
T129 Phosphorylation
T153 Phosphorylation
Y173 Phosphorylation P00519 (ABL1)
Y182 Phosphorylation P00519 (ABL1)
Y188 Phosphorylation P00519 (ABL1)
S189 Phosphorylation
S237 Phosphorylation
T238 Phosphorylation
K240 Acetylation
K240 Sumoylation
K240 Ubiquitination
S251 Phosphorylation P49841 (GSK3B)
T255 Phosphorylation P49841 (GSK3B)
S259 Phosphorylation
K304 Sumoylation
K304 Ubiquitination
S311 Phosphorylation
S312 Phosphorylation
K325 Ubiquitination
T330 Phosphorylation

Research Backgrounds

Function:

Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.

PTMs:

Ubiquitinated by ITCH, leading to its degradation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds DNA as a homodimer or as a heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains).

Family&Domains:

Belongs to the bZIP family. Jun subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

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