Product: Phospho-FRA1 (Thr227) Antibody
Catalog: AF7235
Description: Rabbit polyclonal antibody to Phospho-FRA1 (Thr227)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Sheep
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 43KD; 29kD(Calculated).
Uniprot: P15407
RRID: AB_2843675

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Sheep
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Phospho-FRA1 (Thr227) Antibody detects endogenous levels of FRA1 only when phosphorylated at Thr227.
RRID:
AB_2843675
Cite Format: Affinity Biosciences Cat# AF7235, RRID:AB_2843675.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FOS L1; FOS like antigen 1; Fos related antigen 1; Fos-related antigen 1; FOSL 1; FOSL1; FOSL1 protein; FOSL1_HUMAN; FRA 1; FRA-1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MFRDFGEPGPSSGNGGGYGGPAQPPAAAQAAQQKFHLVPSINTMSGSQELQWMVQPHFLGPSSYPRPLTYPQYSPPQPRPGVIRALGPPPGVRRRPCEQISPEEEERRRVRRERNKLAAAKCRNRRKELTDFLQAETDKLEDEKSGLQREIEELQKQKERLELVLEAHRPICKIPEGAKEGDTGSTSGTSSPPAPCRPVPCISLSPGPVLEPEALHTPTLMTTPSLTPFTPSLVFTYPSTPEPCASAHRKSSSSSGDPSSDPLGSPTLLAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
80
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15407 As Substrate

Site PTM Type Enzyme
R66 Methylation
T69 Phosphorylation
S74 Phosphorylation
R79 Methylation
R84 Methylation
S101 Phosphorylation
K144 Ubiquitination
T183 Phosphorylation
S191 Phosphorylation
T217 Phosphorylation
T223 Phosphorylation Q04759 (PRKCQ)
T227 Phosphorylation
T230 Phosphorylation Q04759 (PRKCQ)
T240 Phosphorylation
S246 Phosphorylation
S251 Phosphorylation
S252 Phosphorylation
S253 Phosphorylation
S254 Phosphorylation
S265 Phosphorylation Q04759 (PRKCQ)
T267 Phosphorylation

Research Backgrounds

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer.

Family&Domains:

Belongs to the bZIP family. Fos subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.