Phospho-Plastin L (Tyr300) Antibody - #AF7224

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Product: Phospho-Plastin L (Tyr300) Antibody
Catalog: AF7224
Description: Rabbit polyclonal antibody to Phospho-Plastin L (Tyr300)
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 70kDa; 70kD(Calculated).
Uniprot: P13796
RRID: AB_2843664

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
Phospho-Plastin L (Tyr300) Antibody detects endogenous levels of Plastin L only when phosphorylated at Tyr300.
RRID:
AB_2843664
Cite Format: Affinity Biosciences Cat# AF7224, RRID:AB_2843664.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CP64; L plastin; L-plastin; Larval cuticle protein 1; LC64P; LCP-1; LCP1; LPL; Lplastin; Lymphocyte cytosolic protein 1; Plastin 2; Plastin-2; PLS2; PLSL_HUMAN;

Immunogens

Immunogen:
Uniprot:

P13796(PLSL_HUMAN) >>Visit HPA database.

Gene(ID):
LCP1(3936)
Expression:
P13796 PLSL_HUMAN:

Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.

Sequence:
MARGSVSDEEMMELREAFAKVDTDGNGYISFNELNDLFKAACLPLPGYRVREITENLMATGDLDQDGRISFDEFIKIFHGLKSTDVAKTFRKAINKKEGICAIGGTSEQSSVGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTNDLFNAVGDGIVLCKMINLSVPDTIDERTINKKKLTPFTIQENLNLALNSASAIGCHVVNIGAEDLKEGKPYLVLGLLWQVIKIGLFADIELSRNEALIALLREGESLEDLMKLSPEELLLRWANYHLENAGCNKIGNFSTDIKDSKAYYHLLEQVAPKGDEEGVPAVVIDMSGLREKDDIQRAECMLQQAERLGCRQFVTATDVVRGNPKLNLAFIANLFNRYPALHKPENQDIDWGALEGETREERTFRNWMNSLGVNPRVNHLYSDLSDALVIFQLYEKIKVPVDWNRVNKPPYPKLGGNMKKLENCNYAVELGKNQAKFSLVGIGGQDLNEGNRTLTLALIWQLMRRYTLNILEEIGGGQKVNDDIIVNWVNETLREAKKSSSISSFKDPKISTSLPVLDLIDAIQPGSINYDLLKTENLNDDEKLNNAKYAISMARKIGARVYALPEDLVEVNPKMVMTVFACLMGKGMKRV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
80
Xenopus
70
Zebrafish
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13796 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation P17612 (PRKACA) , P51812 (RPS6KA3) , Q15418 (RPS6KA1) , Q05655 (PRKCD)
S7 Phosphorylation
K20 Acetylation
K20 Ubiquitination
Y28 Phosphorylation
S30 Phosphorylation
K39 Ubiquitination
Y48 Phosphorylation
T54 Phosphorylation
K76 Acetylation
K76 Ubiquitination
K82 Acetylation
K82 Ubiquitination
K88 Acetylation
K88 Ubiquitination
T89 Phosphorylation
K97 Ubiquitination
S110 Phosphorylation
T114 Phosphorylation
S117 Phosphorylation
Y118 Phosphorylation
S119 Phosphorylation
K123 Ubiquitination
Y124 Phosphorylation
K132 Ubiquitination
K165 Ubiquitination
S170 Phosphorylation
S243 Phosphorylation
S257 Phosphorylation
K263 Ubiquitination
S265 Phosphorylation
Y276 Phosphorylation
K285 Ubiquitination
S290 Phosphorylation
T291 Phosphorylation
K294 Acetylation
K294 Ubiquitination
K297 Acetylation
K297 Ubiquitination
Y299 Phosphorylation
Y300 Phosphorylation
S323 Phosphorylation
T351 Phosphorylation
T353 Phosphorylation
K361 Acetylation
K361 Ubiquitination
Y374 Phosphorylation
K379 Acetylation
K379 Ubiquitination
T399 Phosphorylation
S406 Phosphorylation
Y417 Phosphorylation
Y430 Phosphorylation
K432 Ubiquitination
K434 Methylation
K434 Ubiquitination
K444 Methylation
K444 Ubiquitination
K449 Ubiquitination
K455 Acetylation
K456 Acetylation
K456 Ubiquitination
K468 Ubiquitination
K472 Acetylation
K472 Ubiquitination
S474 Phosphorylation
K542 Acetylation
K542 Ubiquitination
S547 Phosphorylation
T548 Phosphorylation
S549 Phosphorylation
K579 Acetylation
K579 Ubiquitination
K584 Acetylation
K584 Ubiquitination
Y598 Phosphorylation
K610 Ubiquitination
T614 Phosphorylation

Research Backgrounds

Function:

Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.

PTMs:

Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell junction. Cell projection. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Relocalizes to the immunological synapse between peripheral blood T-lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed:17294403). Associated with the actin cytoskeleton at membrane ruffles. Relocalizes to actin-rich cell projections upon serine phosphorylation (PubMed:16636079).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.

Subunit Structure:

Monomer. Interacts with AIF1 (By similarity). Interacts with actin.

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