Phospho-Coagulation Factor III (Ser290) Antibody - #AF7223

(1)   (5)  
Product: Phospho-Coagulation Factor III (Ser290) Antibody
Catalog: AF7223
Description: Rabbit polyclonal antibody to Phospho-Coagulation Factor III (Ser290)
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep
Mol.Wt.: 33kDa; 33kD(Calculated).
Uniprot: P13726
RRID: AB_2843663

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(88%), Bovine(86%), Sheep(86%)
Clonality:
Polyclonal
Specificity:
Phospho-Coagulation Factor III (Ser290) Antibody detects endogenous levels of Coagulation Factor III only when phosphorylated at Ser290.
RRID:
AB_2843663
Cite Format: Affinity Biosciences Cat# AF7223, RRID:AB_2843663.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD142; CD142 antigen; Coagulation factor III (thromboplastin tissue factor); Coagulation factor III; F3; FLJ17960; TF; TF_HUMAN; TFA; Thromboplastin; Tissue factor;

Immunogens

Immunogen:
Uniprot:

P13726(TF_HUMAN) >>Visit HPA database.

Gene(ID):
F3(2152)
Expression:
P13726 TF_HUMAN:

Lung, placenta and pancreas.

Sequence:
METPAWPRVPRPETAVARTLLLGWVFAQVAGASGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFREIFYIIGAVVFVVIILVIILAISLHKCRKAGVGQSWKENSPLNVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Bovine
86
Sheep
86
Rabbit
75
Dog
71
Horse
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13726 As Substrate

Site PTM Type Enzyme
K100 Ubiquitination
S120 Phosphorylation
S285 Phosphorylation Q16539 (MAPK14) , P17252 (PRKCA)
K287 Methylation
K287 Ubiquitination
S290 Phosphorylation P17252 (PRKCA) , Q16539 (MAPK14)
S295 Phosphorylation

Research Backgrounds

Function:

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Lung, placenta and pancreas.

Subunit Structure:

Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII.

Family&Domains:

Belongs to the tissue factor family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

References

1). Investigations on the Changes of Serum Proteins in Rabbits after Trimeresurus stejnegeri Venom Injection via Mass Spectrometry-Based Proteomics. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 35783526)

Application: WB    Species: Rabbit    Sample:

Figure 6 The expression level of TF, p-TF, SLC16A1, and p-SLC16A1 was evaluated by the Western blot assay. Compared to the control group, p-TF/TF was significantly upregulated in the model group. In addition, compared to control, the expression level of p-SLC16A1/SLC16A1 was found significantly suppressed in the model group (p < 0.05).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.