Phospho-TCTP (Ser53) Antibody - #AF7222

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Product: Phospho-TCTP (Ser53) Antibody
Catalog: AF7222
Description: Rabbit polyclonal antibody to Phospho-TCTP (Ser53)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 24KD; 20kD(Calculated).
Uniprot: P13693
RRID: AB_2843662

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 100ul $350 In stock
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Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Phospho-TCTP (Ser53) Antibody detects endogenous levels of TCTP only when phosphorylated at Ser53.
RRID:
AB_2843662
Cite Format: Affinity Biosciences Cat# AF7222, RRID:AB_2843662.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ27337; Fortilin; Histamine releasing factor; Histamine-releasing factor; HRF; Immunoglobulin E-dependent; p02; p23; TCTP; TCTP_HUMAN; tpt1; Translationally controlled tumor protein; Translationally-controlled tumor protein; Tumor protein translationally controlled 1; tumor protein, translationally-controlled 1;

Immunogens

Immunogen:
Uniprot:

P13693(TCTP_HUMAN) >>Visit HPA database.

Gene(ID):
TPT1(7178)
Expression:
P13693 TCTP_HUMAN:

Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.

Sequence:
MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
80
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13693 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
S15 Phosphorylation
Y18 Phosphorylation
K19 Acetylation
K19 Ubiquitination
S37 Phosphorylation
T39 Phosphorylation
S46 Phosphorylation P53350 (PLK1)
S53 Phosphorylation
S64 Phosphorylation P53350 (PLK1)
T65 Phosphorylation
Y88 Phosphorylation
Y91 Phosphorylation
K93 Acetylation
K93 Ubiquitination
Y95 Phosphorylation
K97 Acetylation
K97 Ubiquitination
S98 Phosphorylation
K102 Acetylation
K102 Sumoylation
K102 Ubiquitination
K112 Acetylation
K112 Sumoylation
K112 Ubiquitination
T116 Phosphorylation
K123 Ubiquitination
K130 Methylation
K130 Sumoylation
Y159 Phosphorylation
K164 Ubiquitination
K171 Ubiquitination

Research Backgrounds

Function:

Involved in calcium binding and microtubule stabilization.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.

Subunit Structure:

Interacts with STEAP3.

Family&Domains:

Belongs to the TCTP family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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