Phospho-A-RAF (Ser214) Antibody | Affinity Biosciences

WB
pElisa

1/2

Western blot analysis of extracts from various samples, using Phospho-A-RAF (Ser214) Antibody.

Product:	Phospho-A-RAF (Ser214) Antibody
Catalog:	AF7214
Description:	Rabbit polyclonal antibody to Phospho-A-RAF (Ser214)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	68kDa; 68kD(Calculated).
Uniprot:	P10398
RRID:	AB_2843654

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-A-RAF (Ser214) Antibody detects endogenous levels of A-RAF only when phosphorylated at Ser214.

RRID:

AB_2843654
Cite Format: Affinity Biosciences Cat# AF7214, RRID:AB_2843654.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A raf 1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; Araf; ARaf proto oncogene serine/threonine protein kinase; ARAF_HUMAN; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; Serine/threonine-protein kinase A-Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1 (murine sarcoma 3611 virus);

Immunogens

Immunogen:

Uniprot:

P10398(ARAF_HUMAN) >>Visit HPA database.

Gene(ID):

ARAF(369)

Expression:

P10398 ARAF_HUMAN:

Predominantly in urogenital tissues.

Sequence:

P10398 ARAF_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Rabbit

100

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10398 As Substrate

P10398

Site	PTM Type	Enzyme	Source
S15	Phosphorylation		Uniprot
T20	Phosphorylation		Uniprot
Y24	Phosphorylation		Uniprot
K28	Ubiquitination		Uniprot
Y42	Phosphorylation		Uniprot
Y155	Phosphorylation		Uniprot
S157	Phosphorylation		Uniprot
S162	Phosphorylation		Uniprot
T181	Phosphorylation		Uniprot
S186	Phosphorylation		Uniprot
S212	Phosphorylation		Uniprot
T213	Phosphorylation		Uniprot
S214	Phosphorylation		Uniprot
T215	Phosphorylation		Uniprot
S222	Phosphorylation		Uniprot
T223	Phosphorylation		Uniprot
T224	Phosphorylation		Uniprot
S229	Phosphorylation		Uniprot
S250	Phosphorylation		Uniprot
T253	Phosphorylation		Uniprot
S257	Phosphorylation		Uniprot
S259	Phosphorylation		Uniprot
S262	Phosphorylation		Uniprot
S264	Phosphorylation		Uniprot
S265	Phosphorylation		Uniprot
S269	Phosphorylation		Uniprot
S272	Phosphorylation		Uniprot
S274	Phosphorylation		Uniprot
K282	Ubiquitination		Uniprot
S283	Phosphorylation		Uniprot
K292	Ubiquitination		Uniprot
Y296	Phosphorylation		Uniprot
S299	Phosphorylation	Q13153 (PAK1)	Uniprot
Y301	Phosphorylation	P12931 (SRC)	Uniprot
Y302	Phosphorylation	P12931 (SRC)	Uniprot
K314	Ubiquitination		Uniprot
T318	Phosphorylation		Uniprot
S320	Phosphorylation		Uniprot
T323	Phosphorylation		Uniprot
K339	Ubiquitination		Uniprot
S341	Phosphorylation		Uniprot
T344	Phosphorylation		Uniprot
K352	Ubiquitination		Uniprot
T413	Phosphorylation		Uniprot
Y419	Phosphorylation		Uniprot
K423	Ubiquitination		Uniprot
K431	Ubiquitination		Uniprot
S432	Phosphorylation		Uniprot
T442	Phosphorylation		Uniprot
T452	Phosphorylation		Uniprot
K454	Ubiquitination		Uniprot
T455	Phosphorylation		Uniprot
S458	Phosphorylation		Uniprot
Y526	Phosphorylation		Uniprot
S528	Phosphorylation		Uniprot
S532	Phosphorylation		Uniprot
S535	Phosphorylation		Uniprot
S536	Phosphorylation		Uniprot
K540	Ubiquitination		Uniprot
S547	Phosphorylation		Uniprot
K551	Ubiquitination		Uniprot
S573	Phosphorylation		Uniprot
K576	Ubiquitination		Uniprot
S576	Phosphorylation		Uniprot
S580	Phosphorylation		Uniprot
S582	Phosphorylation		Uniprot
S585	Phosphorylation		Uniprot
T589	Phosphorylation		Uniprot
S600	Phosphorylation		Uniprot

PTMs - P10398 As Enzyme

P10398

Substrate	Site	Source
Q02750 (MAP2K1)	S218	Uniprot
Q02750-1 (MAP2K1)	S222	Uniprot
Q15599 (SLC9A3R2)	S303	Uniprot
Q92934 (BAD)	S75	Uniprot
Q92934 (BAD)	S99	Uniprot
Q92934 (BAD)	S118	Uniprot

Research Backgrounds

P10398_ARAF_HUMAN

Function:

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.

Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.

Tissue Specificity:

Predominantly in urogenital tissues.

Subunit Structure:

Interacts with TH1L/NELFD.

Family&Domains:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Glioma. (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma. (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-A-RAF (Ser214) Antibody - #AF7214