Phospho-KIF20A (Tyr869) Antibody - #AF7164

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Product: Phospho-KIF20A (Tyr869) Antibody
Catalog: AF7164
Description: Rabbit polyclonal antibody to Phospho-KIF20A (Tyr869)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 100kDa; 100kD(Calculated).
Uniprot: O95235
RRID: AB_2843604

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(100%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
Phospho-KIF20A (Tyr869) Antibody detects endogenous levels of KIF20A only when phosphorylated at Tyr869.
RRID:
AB_2843604
Cite Format: Affinity Biosciences Cat# AF7164, RRID:AB_2843604.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AA415432; FLJ21151; GG10 2; GG10_2; KI20A_HUMAN; KIF20A; kinesin family member 20A; Kinesin like protein KIF20A; Kinesin-like protein 174; Kinesin-like protein KIF20A; mitotic kinesin like protein 2; Mitotic kinesin-like protein 2; MKlp2; OTTHUMP00000159481; RAB6 interacting, kinesin like (rabkinesin6); Rab6-interacting kinesin-like protein; RAB6-INTERACTING PROTEIN, KINESIN-LIKE; RAB6KIFL; Rabkinesin 6; Rabkinesin-6;

Immunogens

Immunogen:
Uniprot:

O95235(KI20A_HUMAN) >>Visit HPA database.

Gene(ID):
KIF20A(10112)
Sequence:
MSQGILSPPAGLLSDDDVVVSPMFESTAADLGSVVRKNLLSDCSVVSTSLEDKQQVPSEDSMEKVKVYLRVRPLLPSELERQEDQGCVRIENVETLVLQAPKDSFALKSNERGIGQATHRFTFSQIFGPEVGQASFFNLTVKEMVKDVLKGQNWLIYTYGVTNSGKTHTIQGTIKDGGILPRSLALIFNSLQGQLHPTPDLKPLLSNEVIWLDSKQIRQEEMKKLSLLNGGLQEEELSTSLKRSVYIESRIGTSTSFDSGIAGLSSISQCTSSSQLDETSHRWAQPDTAPLPVPANIRFSIWISFFEIYNELLYDLLEPPSQQRKRQTLRLCEDQNGNPYVKDLNWIHVQDAEEAWKLLKVGRKNQSFASTHLNQNSSRSHSIFSIRILHLQGEGDIVPKISELSLCDLAGSERCKDQKSGERLKEAGNINTSLHTLGRCIAALRQNQQNRSKQNLVPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCASTYDETLHVAKFSAIASQLVHAPPMQLGFPSLHSFIKEHSLQVSPSLEKGAKADTGLDDDIENEADISMYGKEELLQVVEAMKTLLLKERQEKLQLEMHLRDEICNEMVEQMQQREQWCSEHLDTQKELLEEMYEEKLNILKESLTSFYQEEIQERDEKIEELEALLQEARQQSVAHQQSGSELALRRSQRLAASASTQQLQEVKAKLQQCKAELNSTTEELHKYQKMLEPPPSAKPFTIDVDKKLEEGQKNIRLLRTELQKLGESLQSAERACCHSTGAGKLRQALTTCDDILIKQDQTLAELQNNMVLVKLDLRKKAACIAEQYHTVLKLQGQVSAKKRLGTNQENQQPNQQPPGKKPFLRNLLPRTPTCQSSTDCSPYARILRSRRSPLLKSGPFGKKY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
89
Xenopus
86
Zebrafish
57
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95235 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S7 Phosphorylation
S14 Phosphorylation
S21 Phosphorylation
S33 Phosphorylation
K37 Ubiquitination
S41 Phosphorylation
S44 Phosphorylation
S47 Phosphorylation
S49 Phosphorylation
S58 Phosphorylation
S61 Phosphorylation
K102 Ubiquitination
K108 Ubiquitination
S109 Phosphorylation
K150 Ubiquitination
K175 Ubiquitination
T198 Phosphorylation
K215 Ubiquitination
K224 Ubiquitination
S226 Phosphorylation
S240 Phosphorylation
K242 Ubiquitination
S244 Phosphorylation
Y246 Phosphorylation
S249 Phosphorylation
T255 Phosphorylation
S256 Phosphorylation
S259 Phosphorylation
S274 Phosphorylation
K342 Ubiquitination
K357 Ubiquitination
K364 Ubiquitination
S405 Phosphorylation
K425 Ubiquitination
K453 Ubiquitination
S522 Phosphorylation
S528 Phosphorylation
S532 Phosphorylation
K537 Ubiquitination
K540 Ubiquitination
T543 Phosphorylation
S556 Phosphorylation
Y558 Phosphorylation
T572 Phosphorylation
K576 Ubiquitination
K581 Ubiquitination
K625 Ubiquitination
K630 Ubiquitination
S632 Phosphorylation
S635 Phosphorylation
Y637 Phosphorylation
K647 Ubiquitination
S662 Phosphorylation
S668 Phosphorylation
S670 Phosphorylation
S677 Phosphorylation
S683 Phosphorylation
S685 Phosphorylation
T686 Phosphorylation
K693 Ubiquitination
S705 Phosphorylation
T706 Phosphorylation
K712 Ubiquitination
K739 Ubiquitination
K750 Ubiquitination
S754 Phosphorylation
S757 Phosphorylation
K819 Ubiquitination
S825 Phosphorylation
K827 Ubiquitination
T832 Phosphorylation
T857 Phosphorylation
T859 Phosphorylation
S862 Phosphorylation
S863 Phosphorylation
T864 Phosphorylation
S867 Phosphorylation
Y869 Phosphorylation
S875 Phosphorylation
S878 Phosphorylation Q96GD4 (AURKB)
S883 Phosphorylation

Research Backgrounds

Function:

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.

PTMs:

Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.

Subcellular Location:

Golgi apparatus. Cytoplasm>Cytoskeleton>Spindle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

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