Phospho-STAM2 (Tyr371) Antibody | Affinity Biosciences

IHC
pElisa

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AF7158 at 1/100 staining human kidney tissue sections by IHC-P.

AF7158 at 1/100 staining mouse brain tissue sections by IHC-P.

Product:	Phospho-STAM2 (Tyr371) Antibody
Catalog:	AF7158
Description:	Rabbit polyclonal antibody to Phospho-STAM2 (Tyr371)
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	58kDa; 58kD(Calculated).
Uniprot:	O75886
RRID:	AB_2843598

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(90%), Bovine(90%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(80%), Xenopus(90%)

Clonality:

Polyclonal

Specificity:

Phospho-STAM2 (Tyr371) Antibody detects endogenous levels of STAM2 only when phosphorylated at Tyr371.

RRID:

AB_2843598
Cite Format: Affinity Biosciences Cat# AF7158, RRID:AB_2843598.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp564C047; Hbp; Hrs-binding protein; HSE1 homolog; Signal transducing adapter molecule 2; Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2; Signal transducing adaptor molecule 2A; Signal transducing adaptor molecule 2B; Signal transducing adaptor molecule; STAM like protein containing SH3 and ITAM domains 2; STAM-2; Stam2; STAM2_HUMAN; STAM2A; STAM2B;

Immunogens

Immunogen:

Uniprot:

O75886(STAM2_HUMAN) >>Visit HPA database.

Gene(ID):

STAM2(10254)

Expression:

O75886 STAM2_HUMAN:

Ubiquitously expressed.

Sequence:

O75886 STAM2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLFTANPFEQDVEKATNEYNTTEDWSLIMDICDKVGSTPNGAKDCLKAIMKRVNHKVPHVALQALTLLGACVANCGKIFHLEVCSRDFATEVRAVIKNKAHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEGITFPPAGSQTVSAAAKNGTSSNKNKEDEDIAKAIELSLQEQKQQHTETKSLYPSSEIQLNNKVARKVRALYDFEAVEDNELTFKHGEIIIVLDDSDANWWKGENHRGIGLFPSNFVTTNLNIETEAAAVDKLNVIDDDVEEIKKSEPEPVYIDEDKMDRALQVLQSIDPTDSKPDSQDLLDLEDICQQMGPMIDEKLEEIDRKHSELSELNVKVLEALELYNKLVNEAPVYSVYSKLHPPAHYPPASSGVPMQTYPVQSHGGNYMGQSIHQVTVAQSYSLGPDQIGPLRSLPPNVNSSVTAQPAQTSYLSTGQDTVSNPTYMNQNSNLQSATGTTAYTQQMGMSVDMSSYQNTTSNLPQLAGFPVTVPAHPVAQQHTNYHQQPLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Sheep

100

Pig

Bovine

Xenopus

Rabbit

Dog

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75886 As Substrate

O75886

Site	PTM Type	Enzyme	Source
	Ubiquitination		Uniprot
K57	Ubiquitination		Uniprot
K104	Ubiquitination		Uniprot
K122	Ubiquitination		Uniprot
S127	Phosphorylation		Uniprot
K134	Ubiquitination		Uniprot
K156	Ubiquitination		Uniprot
T159	Phosphorylation		Uniprot
S160	Phosphorylation		Uniprot
S161	Phosphorylation		Uniprot
K165	Ubiquitination		Uniprot
K172	Ubiquitination		Uniprot
S177	Phosphorylation		Uniprot
K182	Ubiquitination		Uniprot
K189	Ubiquitination		Uniprot
S190	Phosphorylation		Uniprot
Y192	Phosphorylation		Uniprot
K202	Ubiquitination		Uniprot
K283	Ubiquitination		Uniprot
K284	Ubiquitination		Uniprot
Y291	Phosphorylation	P06241 (FYN)	Uniprot
K296	Ubiquitination		Uniprot
K343	Ubiquitination		Uniprot
S345	Phosphorylation		Uniprot
K363	Ubiquitination		Uniprot
Y371	Phosphorylation	P06241 (FYN)	Uniprot
S372	Phosphorylation		Uniprot
Y374	Phosphorylation	P06241 (FYN)	Uniprot
S375	Phosphorylation		Uniprot
Y418	Phosphorylation		Uniprot

Research Backgrounds

O75886_STAM2_HUMAN

Function:

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).

PTMs:

Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.

Subcellular Location:

Cytoplasm. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 and EPS15. Interacts with JAK2 and JAK3. Interacts with ubiquitinated proteins and the deubiquitinating enzyme USP8/UBPY (By similarity). Interacts (via the via the PxVxL motif) with CBX5; the interaction is direct. Interacts with VPS37C. Interacts with ubiquitin; the interaction is direct. Interacts (via UIM domain) with UBQLN1 (via ubiquitin-like domain) (By similarity).

Family&Domains:

The VHS and UIM domains mediate the interaction with ubiquitinated proteins.

The SH3 domain mediates the interaction with USP8.

Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.

Belongs to the STAM family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

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Phospho-STAM2 (Tyr371) Antibody - #AF7158