Product: Phospho-TNNI3 (Thr143) Antibody
Catalog: AF3214
Description: Rabbit polyclonal antibody to Phospho-TNNI3 (Thr143)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 28kDa; 24kD(Calculated).
Uniprot: P19429
RRID: AB_2834642

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(93%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-TNNI3 (Thr143) Antibody detects endogenous levels of TNNI3 only when phosphorylated at Threonine 143.
RRID:
AB_2834642
Cite Format: Affinity Biosciences Cat# AF3214, RRID:AB_2834642.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cardiac muscle; Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); Cardiomyopathy, familial hypertrophic, 7, included; CMD1FF; CMD2A; CMH7; cTnI; Familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; Tn1; Tni; TNN I3; TNNC 1; TNNC1; TNNI3; TNNI3_HUMAN; Troponin I; Troponin I cardiac; Troponin I cardiac muscle; Troponin I cardiac muscle isoform; Troponin I type 3 cardiac; troponin I, cardiac 3; TroponinI; Ttroponin I type 3 (cardiac);

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Troponin I (TnI) is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle; see also TNNI1 (MIM 191042). The others are troponin T (TnT; see MIM 191041) and troponin C (TnC; see MIM 191040).
Sequence:
MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
93
Xenopus
77
Chicken
69
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19429 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
S6 Phosphorylation
S23 Phosphorylation Q13976 (PRKG1) , Q15139 (PRKD1) , P17612 (PRKACA) , Q05655 (PRKCD) , Q13131 (PRKAA1) , P17252 (PRKCA)
S24 Phosphorylation Q05655 (PRKCD) , P17612 (PRKACA) , P17252 (PRKCA) , Q15139 (PRKD1) , Q13976 (PRKG1) , Q13131 (PRKAA1)
Y26 Phosphorylation
Y29 Phosphorylation
T31 Phosphorylation Q13043 (STK4)
S39 Phosphorylation P17612 (PRKACA)
K40 Methylation
S42 Phosphorylation P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB)
S44 Phosphorylation P05771 (PRKCB) , P17252 (PRKCA) , Q02156 (PRKCE) , P05129 (PRKCG)
T51 Phosphorylation Q13043 (STK4)
K72 Acetylation
S77 Phosphorylation P17252 (PRKCA)
T78 Phosphorylation
Y112 Phosphorylation
T129 Phosphorylation Q13043 (STK4)
T143 Phosphorylation Q05655 (PRKCD) , Q13043 (STK4) , P05771 (PRKCB) , P17252 (PRKCA)
S150 Phosphorylation O75914 (PAK3) , Q13131 (PRKAA1)
S166 Phosphorylation P17612 (PRKACA)
K177 Acetylation
K178 Acetylation
T181 Phosphorylation
S199 Phosphorylation P17252 (PRKCA)

Research Backgrounds

Function:

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

PTMs:

Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).

Subunit Structure:

Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1.

Family&Domains:

Belongs to the troponin I family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

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