Phospho-KIF1B (Ser1057) Antibody - #AF7129

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Product: Phospho-KIF1B (Ser1057) Antibody
Catalog: AF7129
Description: Rabbit polyclonal antibody to Phospho-KIF1B (Ser1057)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 204kd; 204kD(Calculated).
Uniprot: O60333
RRID: AB_2843569

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-KIF1B (Ser1057) Antibody detects endogenous levels of KIF1B only when phosphorylated at Ser1057.
RRID:
AB_2843569
Cite Format: Affinity Biosciences Cat# AF7129, RRID:AB_2843569.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Charcot Marie Tooth neuropathy 2A; CMT 2; CMT 2A; CMT 2A1; CMT2 A; CMT2 A1; CMT2; CMT2A 1; CMT2A; CMT2A1; D4Mil1e; HMSN II; HMSNII; HMSNII, hereditary motor sensory neuropathy II; KIF 1B; KIF1 B; KIF1B p130; KIF1B p204; KIF1Bp130; KIF1Bp204; kinesin family member 1B; Kinesin like protein KIF1B; Klp;

Immunogens

Immunogen:
Uniprot:

O60333(KIF1B_HUMAN) >>Visit HPA database.

Gene(ID):
KIF1B(23095)
Expression:
O60333 KIF1B_HUMAN:

Isoform 3 is abundant in the skeletal muscle. It is also expressed in fetal brain, lung and kidney, and adult heart, placenta, testis, ovary and small intestine. Isoform 2 is abundant in the brain and also expressed in fetal heart, lung, liver and kidney, and adult skeletal muscle, placenta, liver, kidney, heart, spleen, thymus, prostate, testis, ovary, small intestine, colon and pancreas.

Sequence:
MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDNETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGSKYLKDFQNNKHRYLLASENQRPGHFSTASMGSLTSSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERSNSGEVIVTLEPCERSETYVNGKRVSQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEMEKTHEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTVTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDEMEDFDDEAFVDDAGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDFSSVAMTRSGLSLEELRIVEGQGQSSEVITPPEEISRINDLDLKSSTLLDGKMVMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLAFYHVQNIAVEITESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELNSPPQPCRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKPEVDEAAVDAILSLNIISAKYLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMSDTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRERLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSGDIESLVDREKELATKCLQLLTHTFNREFSQVHGSVSDCKLSDISPIGRDPSESSFSSATLTPSSTCPSLVDSRSNSLDQKTPEANSRASSPCPEFEQFQIVPAVETPYLARAGKNEFLNLVPDIEEIRPSSVVSKKGYLHFKEPLYSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDMNDWLYAFNPLLAGTIRSKLSRRCPSQSKY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60333 As Substrate

Site PTM Type Enzyme
S2 Acetylation
K7 Acetylation
R11 Methylation
K243 Ubiquitination
K266 Ubiquitination
S274 Phosphorylation
T276 Phosphorylation
T277 Phosphorylation
T301 Phosphorylation
K355 Ubiquitination
T467 Phosphorylation
T522 Phosphorylation
S527 Phosphorylation
K552 Ubiquitination
S572 Phosphorylation
C600 S-Nitrosylation
K646 Ubiquitination
T647 Phosphorylation
S654 Phosphorylation
K693 Ubiquitination
Y707 Phosphorylation
S709 Phosphorylation
K784 Ubiquitination
S892 Phosphorylation
S893 Phosphorylation
K999 Ubiquitination
S1035 Phosphorylation
Y1040 Phosphorylation
S1054 Phosphorylation
S1057 Phosphorylation
T1075 Phosphorylation
K1089 Ubiquitination
K1156 Ubiquitination
S1162 Phosphorylation
S1211 Phosphorylation
K1228 Ubiquitination
T1241 Phosphorylation
S1416 Phosphorylation
S1432 Phosphorylation
S1435 Phosphorylation
S1454 Phosphorylation
S1487 Phosphorylation
K1507 Ubiquitination
S1573 Phosphorylation
T1592 Phosphorylation
S1598 Phosphorylation
S1603 Phosphorylation
S1605 Phosphorylation
S1610 Phosphorylation
S1613 Phosphorylation
S1620 Phosphorylation
T1630 Phosphorylation
S1632 Phosphorylation
S1641 Phosphorylation
S1643 Phosphorylation
S1645 Phosphorylation
T1650 Phosphorylation
S1655 Phosphorylation
S1658 Phosphorylation
S1659 Phosphorylation
T1675 Phosphorylation
K1720 Acetylation
T1764 Phosphorylation

Research Backgrounds

Function:

Motor for anterograde transport of mitochondria. Has a microtubule plus end-directed motility. Isoform 2 is required for induction of neuronal apoptosis.

Isoform 1 mediates the transport of synaptic vesicles in neuronal cells.

Subcellular Location:

Cytoplasm>Cytoskeleton. Mitochondrion.

Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 3 is abundant in the skeletal muscle. It is also expressed in fetal brain, lung and kidney, and adult heart, placenta, testis, ovary and small intestine. Isoform 2 is abundant in the brain and also expressed in fetal heart, lung, liver and kidney, and adult skeletal muscle, placenta, liver, kidney, heart, spleen, thymus, prostate, testis, ovary, small intestine, colon and pancreas.

Subunit Structure:

Monomer (By similarity). Interacts (via C-terminus end of the kinesin-motor domain) with CHP1; the interaction occurs in a calcium-dependent manner (By similarity). Interacts with KIFBP.

Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Unc-104 subfamily.

Restrictive clause

 

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