Phospho-FANCA (Ser1449) Antibody | Affinity Biosciences

IHC
IF/ICC
pElisa

1/5

AF7113 at 1/100 staining human heart tissue sections by IHC-P.

AF7113 at 1/100 staining rat heart tissue sections by IHC-P.

AF7113 at 1/100 staining mouse kidney tissue sections by IHC-P.

AF7113 staining Hela cells(4h of LPS treatment) by IF/ICC.

peptide-ELISA analysis of AF7113.

Product:	Phospho-FANCA (Ser1449) Antibody
Catalog:	AF7113
Description:	Rabbit polyclonal antibody to Phospho-FANCA (Ser1449)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse
Mol.Wt.:	162kDa; 163kD(Calculated).
Uniprot:	O15360
RRID:	AB_2843553

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(80%), Horse(80%)

Clonality:

Polyclonal

Specificity:

Phospho-FANCA (Ser1449) Antibody detects endogenous levels of FANCA only when phosphorylated at Ser1449.

RRID:

AB_2843553
Cite Format: Affinity Biosciences Cat# AF7113, RRID:AB_2843553.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FA 1; FA; FA H; FA1; FAA; FACA; FAH; Fanca; FANCA_HUMAN; FANCH; Fanconi anemia complementation group A; Fanconi anemia complementation group H; Fanconi anemia group A protein; Fanconi anemia type 1; MGC75158; Protein FACA;

Immunogens

Immunogen:

Uniprot:

O15360(FANCA_HUMAN) >>Visit HPA database.

Gene(ID):

FANCA(2175)

Sequence:

O15360 FANCA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSDSWVPNSASGQDPGGRRRAWAELLAGRVKREKYNPERAQKLKESAVRLLRSHQDLNALLLEVEGPLCKKLSLSKVIDCDSSEAYANHSSSFIGSALQDQASRLGVPVGILSAGMVASSVGQICTAPAETSHPVLLTVEQRKKLSSLLEFAQYLLAHSMFSRLSFCQELWKIQSSLLLEAVWHLHVQGIVSLQELLESHPDMHAVGSWLFRNLCCLCEQMEASCQHADVARAMLSDFVQMFVLRGFQKNSDLRRTVEPEKMPQVTVDVLQRMLIFALDALAAGVQEESSTHKIVRCWFGVFSGHTLGSVISTDPLKRFFSHTLTQILTHSPVLKASDAVQMQREWSFARTHPLLTSLYRRLFVMLSAEELVGHLQEVLETQEVHWQRVLSFVSALVVCFPEAQQLLEDWVARLMAQAFESCQLDSMVTAFLVVRQAALEGPSAFLSYADWFKASFGSTRGYHGCSKKALVFLFTFLSELVPFESPRYLQVHILHPPLVPGKYRSLLTDYISLAKTRLADLKVSIENMGLYEDLSSAGDITEPHSQALQDVEKAIMVFEHTGNIPVTVMEASIFRRPYYVSHFLPALLTPRVLPKVPDSRVAFIESLKRADKIPPSLYSTYCQACSAAEEKPEDAALGVRAEPNSAEEPLGQLTAALGELRASMTDPSQRDVISAQVAVISERLRAVLGHNEDDSSVEISKIQLSINTPRLEPREHMAVDLLLTSFCQNLMAASSVAPPERQGPWAALFVRTMCGRVLPAVLTRLCQLLRHQGPSLSAPHVLGLAALAVHLGESRSALPEVDVGPPAPGAGLPVPALFDSLLTCRTRDSLFFCLKFCTAAISYSLCKFSSQSRDTLCSCLSPGLIKKFQFLMFRLFSEARQPLSEEDVASLSWRPLHLPSADWQRAALSLWTHRTFREVLKEEDVHLTYQDWLHLELEIQPEADALSDTERQDFHQWAIHEHFLPESSASGGCDGDLQAACTILVNALMDFHQSSRSYDHSENSDLVFGGRTGNEDIISRLQEMVADLELQQDLIVPLGHTPSQEHFLFEIFRRRLQALTSGWSVAASLQRQRELLMYKRILLRLPSSVLCGSSFQAEQPITARCEQFFHLVNSEMRNFCSHGGALTQDITAHFFRGLLNACLRSRDPSLMVDFILAKCQTKCPLILTSALVWWPSLEPVLLCRWRRHCQSPLPRELQKLQEGRQFASDFLSPEAASPAPNPDWLSAAALHFAIQQVREENIRKQLKKLDCEREELLVFLFFFSLMGLLSSHLTSNSTTDLPKAFHVCAAILECLEKRKISWLALFQLTESDLRLGRLLLRVAPDQHTRLLPFAFYSLLSYFHEDAAIREEAFLHVAVDMYLKLVQLFVAGDTSTVSPPAGRSLELKGQGNPVELITKARLFLLQLIPRCPKKSFSHVAELLADRGDCDPEVSAALQSRQQAAPDADLSQEPHLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Pig

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15360 As Substrate

O15360

Site	PTM Type	Enzyme	Source
K34	Ubiquitination		Uniprot
K44	Ubiquitination		Uniprot
K70	Ubiquitination		Uniprot
K76	Ubiquitination		Uniprot
S165	Phosphorylation		Uniprot
K249	Ubiquitination		Uniprot
T256	Phosphorylation		Uniprot
K261	Ubiquitination		Uniprot
K293	Ubiquitination		Uniprot
S303	Phosphorylation		Uniprot
S309	Phosphorylation		Uniprot
T323	Phosphorylation		Uniprot
T329	Phosphorylation		Uniprot
K335	Ubiquitination		Uniprot
S337	Phosphorylation		Uniprot
S347	Phosphorylation	Q13131 (PRKAA1)	Uniprot
T351	Phosphorylation	P51955 (NEK2)	Uniprot
S357	Phosphorylation		Uniprot
S421	Phosphorylation		Uniprot
S458	Phosphorylation		Uniprot
Y488	Phosphorylation		Uniprot
K515	Ubiquitination		Uniprot
K522	Ubiquitination		Uniprot
K608	Ubiquitination		Uniprot
K612	Ubiquitination		Uniprot
K631	Ubiquitination		Uniprot
R683	Methylation		Uniprot
K701	Ubiquitination		Uniprot
S849	Phosphorylation		Uniprot
S850	Phosphorylation		Uniprot
S858	Phosphorylation		Uniprot
K921	Sumoylation		Uniprot
K1079	Ubiquitination		Uniprot
S1149	Phosphorylation	P31749 (AKT1)	Uniprot
K1199	Ubiquitination		Uniprot
S1208	Phosphorylation		Uniprot
S1301	Phosphorylation		Uniprot
T1309	Phosphorylation		Uniprot
K1387	Ubiquitination		Uniprot
K1398	Ubiquitination		Uniprot
K1413	Ubiquitination		Uniprot
S1449	Phosphorylation	Q13315 (ATM) , Q13535 (ATR)	Uniprot

Research Backgrounds

O15360_FANCA_HUMAN

Function:

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

PTMs:

Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H.

Subcellular Location:

Nucleus. Cytoplasm.
Note: The major form is nuclear. The minor form is cytoplasmic.

Subunit Structure:

Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCG and FANCL, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. The complex with FANCC and FANCG may also include EIF2AK2 and HSP70. Interacts with FAAP20/C1orf86; interaction is direct.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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Phospho-FANCA (Ser1449) Antibody - #AF7113