Phospho-Semaphorin 4B (Ser809) Antibody - #AF7098

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Product: Phospho-Semaphorin 4B (Ser809) Antibody
Catalog: AF7098
Description: Rabbit polyclonal antibody to Phospho-Semaphorin 4B (Ser809)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 105KD; 93kD(Calculated).
Uniprot: Q9NPR2
RRID: AB_2843538

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(90%), Horse(100%), Sheep(90%), Rabbit(90%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Semaphorin 4B (Ser809) Antibody detects endogenous levels of Semaphorin 4B only when phosphorylated at Ser809.
RRID:
AB_2843538
Cite Format: Affinity Biosciences Cat# AF7098, RRID:AB_2843538.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B; Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain 4B; SEMA4B; SEMAC; Semaphorin 4B; SemC; KIAA1745; MGC131831; SEM4B_HUMAN.

Immunogens

Immunogen:
Uniprot:

Q9NPR2(SEM4B_HUMAN) >>Visit HPA database.

Gene(ID):
SEMA4B(10509)
Sequence:
MLRTAMGLRSWLAAPWGALPPRPPLLLLLLLLLLLQPPPPTWALSPRISLPLGSEERPFLRFEAEHISNYTALLLSRDGRTLYVGAREALFALSSNLSFLPGGEYQELLWGADAEKKQQCSFKGKDPQRDCQNYIKILLPLSGSHLFTCGTAAFSPMCTYINMENFTLARDEKGNVLLEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSLRPTKTESSLNWLQDPAFVASAYIPESLGSLQGDDDKIYFFFSETGQEFEFFENTIVSRIARICKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLSPSPQDWRDTLFYGVFTSQWHRGTTEGSAVCVFTMKDVQRVFSGLYKEVNRETQQWYTVTHPVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRMLLLQPQARYQRVAVHRVPGLHHTYDVLFLGTGDGRLHKAVSVGPRVHIIEELQIFSSGQPVQNLLLDTHRGLLYAASHSGVVQVPMANCSLYRSCGDCLLARDPYCAWSGSSCKHVSLYQPQLATRPWIQDIEGASAKDLCSASSVVSPSFVPTGEKPCEQVQFQPNTVNTLACPLLSNLATRLWLRNGAPVNASASCHVLPTGDLLLVGTQQLGEFQCWSLEEGFQQLVASYCPEVVEDGVADQTDEGGSVPVIISTSRVSAPAGGKASWGADRSYWKEFLVMCTLFVLAVLLPVLFLLYRHRNSMKVFLKQGECASVHPKTCPVVLPPETRPLNGLGPPSTPLDHRGYQSLSDSPPGSRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Xenopus
100
Bovine
90
Sheep
90
Rabbit
90
Chicken
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPR2 As Substrate

Site PTM Type Enzyme
N64 N-Glycosylation
Y65 Phosphorylation
T66 Phosphorylation
K403 Acetylation
N405 N-Glycosylation
S694 Phosphorylation
K740 Ubiquitination
K744 Ubiquitination
T764 Phosphorylation
S774 Phosphorylation
T775 Phosphorylation
Y782 Phosphorylation
S784 Phosphorylation
S786 Phosphorylation
S788 Phosphorylation
T796 Phosphorylation
K800 Ubiquitination
S804 Phosphorylation
S808 Phosphorylation
S813 Phosphorylation
S825 Phosphorylation
S830 Phosphorylation

Research Backgrounds

Function:

Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the semaphorin family.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

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