Phospho-Annexin A2 (Tyr24) Antibody - #AF7096

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Product: Phospho-Annexin A2 (Tyr24) Antibody
Catalog: AF7096
Description: Rabbit polyclonal antibody to Phospho-Annexin A2 (Tyr24)
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 37kDa; 39kD(Calculated).
Uniprot: P07355
RRID: AB_2843536

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 100ul $350 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
Phospho-Annexin A2 (Tyr24) Antibody detects endogenous levels of Annexin A2 only when phosphorylated at Tyr24.
RRID:
AB_2843536
Cite Format: Affinity Biosciences Cat# AF7096, RRID:AB_2843536.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Annexin A2; Annexin II; Annexin II, heavy chain; Annexin-2; ANX 2; ANX2; ANX2L4; ANXA2; ANXA2_HUMAN; arylsulfatase B; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide (p36); Calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; Chromobindin-8; Epididymis secretory protein Li 270; HEL S 270; LIP2; Lipocortin II; LPC2; LPC2D; p36; P36 protein; PAP-IV; Placental anticoagulant protein IV; Protein I;

Immunogens

Immunogen:

A synthesized peptide derived from human Annexin A2 around the phosphorylation site of Tyr24.

Uniprot:

P07355(ANXA2_HUMAN) >>Visit HPA database.

Gene(ID):
ANXA2(302)
Sequence:
MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
90
Horse
90
Chicken
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.

PTMs:

Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.

ISGylated.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix>Basement membrane. Melanosome.
Note: In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Belongs to the annexin family.

References

1). (20S) Ginsenoside Rh2 Inhibits STAT3/VEGF Signaling by Targeting Annexin A2. International Journal of Molecular Sciences, 2021 (PubMed: 34502195) [IF=5.6]

Application: WB    Species: Human    Sample: HepG2 cells

igure 1. Anxa2 regulated the expression of VEGFs. HepG2 cells were treated with three different Anxa2-shRNAs. (A) Protein level and phosphorylation status of STAT3 and Anxa2 were determined by immuno-blotting. (B) The activation of STAT3 was determined via reporter gene assay. (C) The expression of STAT3 target genes was determined by qRT-PCR. (D) The protein level of four VEGFs was determined by immuno-blotting. (E) The concentration of four VEGFs in cell-free supernatant was determined by ELISA. All experiments were performed with triple replicant.
2). GNAQ T96S mutation abrogates the ability of wild‐type GNAQ to induce apoptosis by phosphorylating annexin A2 in natural killer/T cell lymphoma. Cancer Science, 2022 (PubMed: 35293080) [IF=4.5]

Application: WB    Species: Mice    Sample: NK cells

FIGURE 4 GNAQ T96S peptide inhibitor induces apoptosis by competing with annexin A2 (ANXA2) binding to GNAQ T96S in natural killer cells. (A) A schematic diagram showing the different domains of GNAQ. (B) Identification of the interaction between different domains of GNAQ and ANXA2 by co‐immunoprecipitation (IP). M or R refers to the host of antibody. (C) GNAQ T96S peptide blocks the binding of GNAQ to ANXA2 in a dose‐dependent manner. (D) GNAQ T96S peptide significantly induces apoptosis in NK cells. (E) GNAQ peptide affects several signaling pathways, including the AKT, ERK, and ANXA2 signaling pathways. The experiment was independently repeated three times
3). A novel cystathionine γ-lyase inhibitor, I194496, inhibits the growth and metastasis of human TNBC via downregulating multiple signaling pathways. Scientific Reports, 2021 (PubMed: 33903672) [IF=3.8]

Application: WB    Species: Human    Sample: TNBC cells

Figure 6 I194496 inhibits the Anxa2/STAT3 and VEGF/FAK/Paxillin signalling pathways in human TNBC cells. (A, B) The effect of I194496 on Anxa2/STAT3 pathway in MDA-MB-231 cells. *P 

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