Phospho-ARHGAP42 (Tyr376) Antibody - #AF7094
(2)
| Product: | Phospho-ARHGAP42 (Tyr376) Antibody |
| Catalog: | AF7094 |
| Description: | Rabbit polyclonal antibody to Phospho-ARHGAP42 (Tyr376) |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 100~120kDa; 99kD(Calculated). |
| Uniprot: | A6NI28 |
| RRID: | AB_2843534 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-ARHGAP42 (Tyr376) Antibody detects endogenous levels of ARHGAP42 only when phosphorylated at Tyr376.
RRID:
AB_2843534
Cite Format: Affinity Biosciences Cat# AF7094, RRID:AB_2843534.
Cite Format: Affinity Biosciences Cat# AF7094, RRID:AB_2843534.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ARHGAP42; FLJ32810; GRAF3; RHG42_HUMAN; Rho GTPase-activating protein 10-like; Rho GTPase-activating protein 42; Rho-type GTPase-activating protein 42;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- A6NI28 RHG42_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLPTLEFSDSYLDSPDFRERLQCHEIELERTNKFIKELIKDGSLLIGALRNLSMAVQKFSQSLQDFQFECIGDAETDDEISIAQSLKEFARLLIAVEEERRRLIQNANDVLIAPLEKFRKEQIGAAKDGKKKFDKESEKYYSILEKHLNLSAKKKESHLQEADTQIDREHQNFYEASLEYVFKIQEVQEKKKFEFVEPLLSFLQGLFTFYHEGYELAQEFAPYKQQLQFNLQNTRNNFESTRQEVERLMQRMKSANQDYRPPSQWTMEGYLYVQEKRPLGFTWIKHYCTYDKGSKTFTMSVSEMKSSGKMNGLVTSSPEMFKLKSCIRRKTDSIDKRFCFDIEVVERHGIITLQAFSEANRKLWLEAMDGKEPIYTLPAIISKKEEMYLNEAGFNFVRKCIQAVETRGITILGLYRIGGVNSKVQKLMNTTFSPKSPPDIDIDIELWDNKTITSGLKNYLRCLAEPLMTYKLHKDFIIAVKSDDQNYRVEAVHALVHKLPEKNREMLDILIKHLVKVSLHSQQNLMTVSNLGVIFGPTLMRAQEETVAAMMNIKFQNIVVEILIEHYEKIFHTAPDPSIPLPQPQSRSGSRRTRAICLSTGSRKPRGRYTPCLAEPDSDSYSSSPDSTPMGSIESLSSHSSEQNSTTKSASCQPREKSGGIPWIATPSSSNGQKSLGLWTTSPESSSREDATKTDAESDCQSVASVTSPGDVSPPIDLVKKEPYGLSGLKRASASSLRSISAAEGNKSYSGSIQSLTSVGSKETPKASPNPDLPPKMCRRLRLDTASSNGYQRPGSVVAAKAQLFENVGSPKPVSSGRQAKAMYSCKAEHSHELSFPQGAIFSNVYPSVEPGWLKATYEGKTGLVPENYVVFL
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - A6NI28 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T32 | Phosphorylation | Uniprot | |
| K128 | Acetylation | Uniprot | |
| K131 | Acetylation | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| T332 | Phosphorylation | Uniprot | |
| Y376 | Phosphorylation | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| S587 | Phosphorylation | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| S683 | Phosphorylation | Uniprot | |
| S728 | Phosphorylation | Uniprot | |
| K731 | Methylation | Uniprot | |
| S734 | Phosphorylation | Uniprot | |
| S740 | Phosphorylation | Uniprot | |
| S742 | Phosphorylation | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| S753 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| T786 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot | |
| S789 | Phosphorylation | Uniprot | |
| Y792 | Phosphorylation | Uniprot | |
| S797 | Phosphorylation | Uniprot | |
| S811 | Phosphorylation | Uniprot | |
| T858 | Phosphorylation | Uniprot | |
| Y870 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
May influence blood pressure by functioning as a GTPase-activating protein for RHOA in vascular smooth muscle.
Tissue Specificity:
Highly and selectively expressed in smooth muscle cells.
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