Phospho-SMYD2 (Ser283) Antibody | Affinity Biosciences

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Product:	Phospho-SMYD2 (Ser283) Antibody
Catalog:	AF7091
Description:	Rabbit polyclonal antibody to Phospho-SMYD2 (Ser283)
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.:	49kDa; 50kD(Calculated).
Uniprot:	Q9NRG4
RRID:	AB_2843531

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(90%), Bovine(90%), Horse(90%), Rabbit(90%), Dog(100%), Chicken(80%)

Clonality:

Polyclonal

Specificity:

Phospho-SMYD2 (Ser283) Antibody detects endogenous levels of SMYD2 only when phosphorylated at Ser283.

RRID:

AB_2843531
Cite Format: Affinity Biosciences Cat# AF7091, RRID:AB_2843531.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Histone methyltransferase SMYD2; HSKM B; HSKM-B; HSKMB; KMT3C; Lysine N-methyltransferase 3C; MGC119305; N lysine methyltransferase SMYD2; N-lysine methyltransferase SMYD2; SET and MYND domain containing 2; SET and MYND domain containing protein 2; SET and MYND domain-containing protein 2; Smyd2; SMYD2_HUMAN; Zinc finger MYND domain containing 14; ZMYND14;

Immunogens

Immunogen:

Uniprot:

Q9NRG4(SMYD2_HUMAN) >>Visit HPA database.

Gene(ID):

SMYD2(56950)

Sequence:

Q9NRG4 SMYD2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRAEGLGGLERFCSPGKGRGLRALQPFQVGDLLFSCPAYAYVLTVNERGNHCEYCFTRKEGLSKCGRCKQAFYCNVECQKEDWPMHKLECSPMVVFGENWNPSETVRLTARILAKQKIHPERTPSEKLLAVKEFESHLDKLDNEKKDLIQSDIAALHHFYSKHLGFPDNDSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIKPGEEVFTSYIDLLYPTEDRNDRLRDSYFFTCECQECTTKDKDKAKVEIRKLSDPPKAEAIRDMVRYARNVIEEFRRAKHYKSPSELLEICELSQEKMSSVFEDSNVYMLHMMYQAMGVCLYMQDWEGALQYGQKIIKPYSKHYPLYSLNVASMWLKLGRLYMGLEHKAAGEKALKKAIAIMEVAHGKDHPYISEIKQEIESH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Horse

Bovine

Rabbit

Chicken

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NRG4 As Substrate

Q9NRG4

Site	PTM Type	Source
S35	Phosphorylation	Uniprot
T44	Phosphorylation	Uniprot
K80	Ubiquitination	Uniprot
K127	Ubiquitination	Uniprot
K132	Ubiquitination	Uniprot
K145	Ubiquitination	Uniprot
K146	Ubiquitination	Uniprot
S283	Phosphorylation	Uniprot
K312	Ubiquitination	Uniprot
K398	Methylation	Uniprot
K418	Ubiquitination	Uniprot
K427	Ubiquitination	Uniprot

Research Backgrounds

Q9NRG4_SMYD2_HUMAN

Function:

Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.

Subcellular Location:

Cytoplasm>Cytosol. Nucleus.

Subunit Structure:

Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1 (By similarity). Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1.

Family&Domains:

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Phospho-SMYD2 (Ser283) Antibody - #AF7091