Phospho-BACH1 (Ser445) Antibody - #AF7089

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Product: Phospho-BACH1 (Ser445) Antibody
Catalog: AF7089
Description: Rabbit polyclonal antibody to Phospho-BACH1 (Ser445)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 105kDa; 82kD(Calculated).
Uniprot: O14867
RRID: AB_2843529

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-BACH1 (Ser445) Antibody detects endogenous levels of BACH1 only when phosphorylated at Ser445.
RRID:
AB_2843529
Cite Format: Affinity Biosciences Cat# AF7089, RRID:AB_2843529.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BACH 1; BACH 1.3; Bach1; BACH1_HUMAN; Basic leucine zipper transcription factor 1; basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homolog 1; BTB and CNC homology 1; BTB and CNC homology 1, basic leucine zipper transcription factor 1; HA2303; OTTHUMP00000096563; OTTHUMP00000096564; Transcription regulator protein BACH1;

Immunogens

Immunogen:
Uniprot:

O14867(BACH1_HUMAN) >>Visit HPA database.

Gene(ID):
BACH1(571)
Sequence:
MSLSENSVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRSVLAACSSYFHSRIVGQADGELNITLPEEVTVKGFEPLIQFAYTAKLILSKENVDEVCKCVEFLSVHNIEESCFQFLKFKFLDSTADQQECPRKKCFSSHCQKTDLKLSLLDQRDLETDEVEEFLENKNVQTPQCKLRRYQGNAKASPPLQDSASQTYESMCLEKDAALALPSLCPKYRKFQKAFGTDRVRTGESSVKDIHASVQPNERSENECLGGVPECRDLQVMLKCDESKLAMEPEETKKDPASQCPTEKSEVTPFPHNSSIDPHGLYSLSLLHTYDQYGDLNFAGMQNTTVLTEKPLSGTDVQEKTFGESQDLPLKSDLGTREDSSVASSDRSSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLLKERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSFLISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQMSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
67
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14867 As Substrate

Site PTM Type Enzyme
K100 Ubiquitination
K129 Ubiquitination
K156 Ubiquitination
S158 Phosphorylation
K177 Ubiquitination
T181 Phosphorylation
K185 Ubiquitination
S196 Phosphorylation
K232 Ubiquitination
T236 Phosphorylation
K247 Ubiquitination
K359 Ubiquitination
T360 Phosphorylation
S364 Phosphorylation
K370 Ubiquitination
S379 Phosphorylation
S388 Phosphorylation
S417 Phosphorylation
S445 Phosphorylation
S504 Phosphorylation
T506 Phosphorylation
S543 Phosphorylation
K549 Ubiquitination
T551 Phosphorylation
K578 Ubiquitination
K596 Ubiquitination
K614 Ubiquitination
K626 Ubiquitination
K655 Ubiquitination
S688 Phosphorylation
C715 S-Nitrosylation
S718 Phosphorylation

Research Backgrounds

Function:

Transcriptional regulator that acts as repressor or activator, depending on the context. Binds to NF-E2 DNA binding sites. Plays important roles in coordinating transcription activation and repression by MAFK (By similarity). Together with MAF, represses the transcription of genes under the control of the NFE2L2 oxidative stress pathway.

PTMs:

Ubiquitinated by the SCF(FBXL17) complex, leading to its degradation by the proteasome.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer of BACH1 and MAFK.

Family&Domains:

Belongs to the bZIP family. CNC subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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