NM23 Antibody | Affinity Biosciences

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AF0222 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

Product:	NM23 Antibody
Catalog:	AF0222
Description:	Rabbit polyclonal antibody to NM23
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Rabbit, Dog
Mol.Wt.:	23kDa; 17kD(Calculated).
Uniprot:	P22392
RRID:	AB_2833397

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(93%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

NM23 Antibody detects endogenous levels of total NM23.

RRID:

AB_2833397
Cite Format: Affinity Biosciences Cat# AF0222, RRID:AB_2833397.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C myc purine binding transcription factor PUF; C myc transcription factor; C-myc purine-binding transcription factor PUF; epididymis secretory sperm binding protein Li 155an; HEL-S-155an; Histidine protein kinase NDKB; MGC111212; MGC2212; NDK B; NDKB; NDKB_HUMAN; NDP kinase B; NDPK B; NDPKB; NM23 H2; nm23-H2; NM23B; NME/NM23 nucleoside diphosphate kinase 2; nme2; Non metastatic cells 2, protein (NM23B) expressed in; non-metastatic cells 2, protein (NM23) expressed in; Nucleoside diphosphate kinase B; Nucleotide diphosphate kinase B; PUF;

Immunogens

Immunogen:

Uniprot:

P22392(NDKB_HUMAN) >>Visit HPA database.

Gene(ID):

NME2(4831)

Expression:

P22392 NDKB_HUMAN:

Isoform 1 and isoform 3 are ubiquitously expressed.

Description:

NM23 nucleoside diphosphate kinase A. Major role in the synthesis of nucleoside triphosphates other than ATP. Holoenzyme is a hexamer of the A and B subunits. Mutations have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been described.

Sequence:

P22392 NDKB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVYE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Rabbit

100

Bovine

Xenopus

Horse

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22392 As Substrate

P22392

Site	PTM Type	Enzyme	Source
R6	Methylation		Uniprot
K12	Acetylation		Uniprot
K12	Ubiquitination		Uniprot
R18	Methylation		Uniprot
K26	Acetylation		Uniprot
K26	Ubiquitination		Uniprot
K31	Acetylation		Uniprot
K31	Ubiquitination		Uniprot
K39	Acetylation		Uniprot
K39	Ubiquitination		Uniprot
R42	Methylation		Uniprot
S44	Phosphorylation	P22392 (NME2)	Uniprot
K49	Acetylation		Uniprot
K49	Ubiquitination		Uniprot
Y52	Phosphorylation		Uniprot
K56	Acetylation		Uniprot
K56	Ubiquitination		Uniprot
R58	Methylation		Uniprot
K66	Acetylation		Uniprot
K66	Ubiquitination		Uniprot
K85	Acetylation		Uniprot
K85	Ubiquitination		Uniprot
R88	Methylation		Uniprot
T94	Phosphorylation	P06493 (CDK1)	Uniprot
S99	Phosphorylation		Uniprot
K100	Acetylation		Uniprot
K100	Methylation		Uniprot
K100	Sumoylation		Uniprot
K100	Ubiquitination		Uniprot
T103	Phosphorylation		Uniprot
R114	Methylation		Uniprot
H118	Phosphorylation	P22392 (NME2)	Uniprot
S120	Phosphorylation		Uniprot
S122	Phosphorylation		Uniprot
K124	Acetylation		Uniprot
K124	Sumoylation		Uniprot
K124	Ubiquitination		Uniprot
K128	Acetylation		Uniprot
K128	Ubiquitination		Uniprot
S131	Phosphorylation		Uniprot
K135	Ubiquitination		Uniprot
Y142	Phosphorylation		Uniprot
K143	Acetylation		Uniprot
K143	Ubiquitination		Uniprot
Y151	Phosphorylation		Uniprot

PTMs - P22392 As Enzyme

P22392

Substrate	Site	Source
O15554 (KCNN4)	H358	Uniprot
P22392 (NME2)	S44	Uniprot
P22392-1 (NME2)	H118	Uniprot
P22392-2 (NME2)	S159	Uniprot
P22392-2 (NME2)	H233	Uniprot

Research Backgrounds

P22392_NDKB_HUMAN

Function:

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity). Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically. Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III(1) region of the MYC gene promoter. Does not bind to duplex NHE III(1). Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not. Exhibits histidine protein kinase activity.

Subcellular Location:

Cytoplasm. Nucleus. Cell projection>Lamellipodium. Cell projection>Ruffle.
Note: Isoform 3 is mainly cytoplasmic and isoform 1 and isoform 3 are excluded from the nucleolus. Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates.

Tissue Specificity:

Isoform 1 and isoform 3 are ubiquitously expressed.

Subunit Structure:

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with CAPN8 (By similarity). Interacts with AKAP13. Interacts ITGB1BP1 (via C-terminal domain region).

Family&Domains:

Belongs to the NDK family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

NM23 Antibody - #AF0222