Product: NM23 Antibody
Catalog: AF0222
Description: Rabbit polyclonal antibody to NM23
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Rabbit, Dog
Mol.Wt.: 23kDa; 17kD(Calculated).
Uniprot: P22392
RRID: AB_2833397

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(93%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NM23 Antibody detects endogenous levels of total NM23.
RRID:
AB_2833397
Cite Format: Affinity Biosciences Cat# AF0222, RRID:AB_2833397.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C myc purine binding transcription factor PUF; C myc transcription factor; C-myc purine-binding transcription factor PUF; epididymis secretory sperm binding protein Li 155an; HEL-S-155an; Histidine protein kinase NDKB; MGC111212; MGC2212; NDK B; NDKB; NDKB_HUMAN; NDP kinase B; NDPK B; NDPKB; NM23 H2; nm23-H2; NM23B; NME/NM23 nucleoside diphosphate kinase 2; nme2; Non metastatic cells 2, protein (NM23B) expressed in; non-metastatic cells 2, protein (NM23) expressed in; Nucleoside diphosphate kinase B; Nucleotide diphosphate kinase B; PUF;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P22392 NDKB_HUMAN:

Isoform 1 and isoform 3 are ubiquitously expressed.

Description:
NM23 nucleoside diphosphate kinase A. Major role in the synthesis of nucleoside triphosphates other than ATP. Holoenzyme is a hexamer of the A and B subunits. Mutations have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been described.
Sequence:
MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVYE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Bovine
93
Xenopus
79
Horse
0
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22392 As Substrate

Site PTM Type Enzyme
R6 Methylation
K12 Acetylation
K12 Ubiquitination
R18 Methylation
K26 Acetylation
K26 Ubiquitination
K31 Acetylation
K31 Ubiquitination
K39 Acetylation
K39 Ubiquitination
R42 Methylation
S44 Phosphorylation P22392 (NME2)
K49 Acetylation
K49 Ubiquitination
Y52 Phosphorylation
K56 Acetylation
K56 Ubiquitination
R58 Methylation
K66 Acetylation
K66 Ubiquitination
K85 Acetylation
K85 Ubiquitination
R88 Methylation
T94 Phosphorylation P06493 (CDK1)
S99 Phosphorylation
K100 Acetylation
K100 Methylation
K100 Sumoylation
K100 Ubiquitination
T103 Phosphorylation
R114 Methylation
H118 Phosphorylation P22392 (NME2)
S120 Phosphorylation
S122 Phosphorylation
K124 Acetylation
K124 Sumoylation
K124 Ubiquitination
K128 Acetylation
K128 Ubiquitination
S131 Phosphorylation
K135 Ubiquitination
Y142 Phosphorylation
K143 Acetylation
K143 Ubiquitination
Y151 Phosphorylation

PTMs - P22392 As Enzyme

Substrate Site Source
O15554 (KCNN4) H358 Uniprot
P22392 (NME2) S44 Uniprot
P22392-1 (NME2) H118 Uniprot
P22392-2 (NME2) S159 Uniprot
P22392-2 (NME2) H233 Uniprot

Research Backgrounds

Function:

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity). Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically. Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III(1) region of the MYC gene promoter. Does not bind to duplex NHE III(1). Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not. Exhibits histidine protein kinase activity.

Subcellular Location:

Cytoplasm. Nucleus. Cell projection>Lamellipodium. Cell projection>Ruffle.
Note: Isoform 3 is mainly cytoplasmic and isoform 1 and isoform 3 are excluded from the nucleolus. Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 and isoform 3 are ubiquitously expressed.

Subunit Structure:

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with CAPN8 (By similarity). Interacts with AKAP13. Interacts ITGB1BP1 (via C-terminal domain region).

Family&Domains:

Belongs to the NDK family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

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