Phospho-TXN (Thr100) Antibody | Affinity Biosciences

IF/ICC
pElisa

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AF7077 staining Hela cells(4h of LPS treatment) by IF/ICC.

Product:	Phospho-TXN (Thr100) Antibody
Catalog:	AF7077
Description:	Rabbit polyclonal antibody to Phospho-TXN (Thr100)
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	12kDa; 12kD(Calculated).
Uniprot:	P10599
RRID:	AB_2843517

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(80%), Sheep(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

Phospho-TXN (Thr100) Antibody detects endogenous levels of TXN only when phosphorylated at Thr100.

RRID:

AB_2843517
Cite Format: Affinity Biosciences Cat# AF7077, RRID:AB_2843517.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADF; ATL derived factor; ATL-derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; testicular tissue protein Li 199; THIO_HUMAN; Thioredoxin; thioredoxin delta 3; TRDX; TRX 1; Trx; TRX1; TXN; TXN delta 3; TXN protein; zgc:92903;

Immunogens

Immunogen:

Uniprot:

P10599(THIO_HUMAN) >>Visit HPA database.

Gene(ID):

TXN(7295)

Sequence:

P10599 THIO_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATINELV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Rabbit

100

Horse

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10599 As Substrate

P10599

Site	PTM Type	Enzyme	Source
K3	Acetylation		Uniprot
K3	Ubiquitination		Uniprot
K8	Acetylation		Uniprot
K8	Methylation		Uniprot
K8	Ubiquitination		Uniprot
T9	Phosphorylation		Uniprot
T30	Phosphorylation		Uniprot
K39	Acetylation		Uniprot
K39	Ubiquitination		Uniprot
S44	Phosphorylation		Uniprot
S46	Phosphorylation		Uniprot
K48	Methylation		Uniprot
C62	S-Nitrosylation		Uniprot
S67	Phosphorylation		Uniprot
C69	S-Nitrosylation		Uniprot
C73	S-Nitrosylation		Uniprot
T76	Phosphorylation		Uniprot
K81	Acetylation		Uniprot
K85	Ubiquitination		Uniprot
S90	Phosphorylation		Uniprot
K94	Acetylation		Uniprot
K94	Ubiquitination		Uniprot
K96	Ubiquitination		Uniprot
T100	Phosphorylation	P17252 (PRKCA)	Uniprot

Research Backgrounds

P10599_THIO_HUMAN

Function:

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

PTMs:

In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.

In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.

Subcellular Location:

Nucleus. Cytoplasm. Secreted.
Note: Translocates from the cytoplasm into the nucleus after phorbol 12-myristate 13-acetate induction (PMA) (PubMed:9108029). Predominantly in the cytoplasm in non irradiated cells (PubMed:11118054). Radiation induces translocation of TRX from the cytoplasm to the nucleus (PubMed:11118054). Secreted by a leaderless secretory pathway (PubMed:1332947).

Subunit Structure:

Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner.

Family&Domains:

Belongs to the thioredoxin family.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

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Phospho-TXN (Thr100) Antibody - #AF7077