Phospho-FKBP4 (Tyr220) Antibody - #AF7065

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Product: Phospho-FKBP4 (Tyr220) Antibody
Catalog: AF7065
Description: Rabbit polyclonal antibody to Phospho-FKBP4 (Tyr220)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 51kDa; 52kD(Calculated).
Uniprot: Q02790
RRID: AB_2843505

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-FKBP4 (Tyr220) Antibody detects endogenous levels of FKBP4 only when phosphorylated at Tyr220.
RRID:
AB_2843505
Cite Format: Affinity Biosciences Cat# AF7065, RRID:AB_2843505.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

51 kDa FK506-binding protein; 52 kDa FK506 binding protein; 52 kDa FK506-binding protein; 52 kDa FKBP; 59 kDa immunophilin; FK506 binding protein 4; FK506-binding protein 4; FKBP 4; FKBP 52; FKBP 59; FKBP-4; FKBP-52; FKBP4; FKBP4_HUMAN; FKBP51; FKBP52 protein; FKBP59; HBI; Hsp 56; HSP binding immunophilin; HSP-binding immunophilin; Hsp56; Immunophilin FKBP52; N-terminally processed; p52; p59; p59 protein; Peptidyl prolyl cis trans isomerase; Peptidyl-prolyl cis-trans isomerase FKBP4; Peptidylprolyl cis trans isomerase; PPIase; PPIase FKBP4; Rotamase; T cell FK506 binding protein 59kD;

Immunogens

Immunogen:
Uniprot:

Q02790(FKBP4_HUMAN) >>Visit HPA database.

Gene(ID):
FKBP4(2288)
Expression:
Q02790 FKBP4_HUMAN:

Widely expressed.

Sequence:
MTAEEMKATESGAQSAPLPMEGVDISPKQDEGVLKVIKREGTGTEMPMIGDRVFVHYTGWLLDGTKFDSSLDRKDKFSFDLGKGEVIKAWDIAIATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGEDLTEEEDGGIIRRIQTRGEGYAKPNEGAIVEVALEGYYKDKLFDQRELRFEIGEGENLDLPYGLERAIQRMEKGEHSIVYLKPSYAFGSVGKEKFQIPPNAELKYELHLKSFEKAKESWEMNSEEKLEQSTIVKERGTVYFKEGKYKQALLQYKKIVSWLEYESSFSNEEAQKAQALRLASHLNLAMCHLKLQAFSAAIESCNKALELDSNNEKGLFRRGEAHLAVNDFELARADFQKVLQLYPNNKAAKTQLAVCQQRIRRQLAREKKLYANMFERLAEEENKAKAEASSGDHPTDTEMKEEQKSNTAGSQSQVETEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Xenopus
57
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02790 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
S15 Phosphorylation
S26 Phosphorylation
K28 Acetylation
K28 Ubiquitination
K35 Ubiquitination
R52 Methylation
K74 Ubiquitination
K76 Acetylation
K76 Ubiquitination
S78 Phosphorylation
K83 Ubiquitination
K88 Ubiquitination
K108 Ubiquitination
S115 Phosphorylation
S118 Phosphorylation
T143 Phosphorylation P68400 (CSNK2A1)
Y161 Phosphorylation
K181 Ubiquitination
S217 Phosphorylation
Y220 Phosphorylation
K222 Acetylation
K222 Ubiquitination
Y225 Phosphorylation
S229 Phosphorylation
K232 Acetylation
K244 Ubiquitination
K250 Ubiquitination
S258 Phosphorylation
S263 Phosphorylation
K274 Acetylation
K274 Ubiquitination
K282 Acetylation
K282 Ubiquitination
Y286 Phosphorylation
K287 Ubiquitination
Y293 Phosphorylation
K294 Acetylation
K294 Ubiquitination
S298 Phosphorylation
S305 Phosphorylation
K313 Ubiquitination
S336 Phosphorylation
S341 Phosphorylation
K344 Ubiquitination
S350 Phosphorylation
K354 Acetylation
K354 Ubiquitination
R359 Methylation
R373 Methylation
K378 Acetylation
K378 Ubiquitination
K387 Ubiquitination
K390 Ubiquitination
C396 S-Nitrosylation
K409 Ubiquitination
Y411 Phosphorylation
R417 Methylation
K424 Ubiquitination
K426 Ubiquitination
S430 Phosphorylation
S431 Phosphorylation
T436 Phosphorylation
T438 Phosphorylation
K441 Sumoylation
K441 Ubiquitination
T448 Phosphorylation
S451 Phosphorylation
S453 Phosphorylation

Research Backgrounds

Function:

Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.

PTMs:

Phosphorylation by CK2 results in loss of HSP90 binding activity.

Subcellular Location:

Cytoplasm>Cytosol. Mitochondrion. Nucleus. Cytoplasm>Cytoskeleton. Cell projection>Axon.
Note: Shuttles from mitochondria to nucleus; co-localizes in mitochondria with the glucocorticoid receptor (PubMed:21730050). Colocalized with MAPT/TAU in the distal part of the primary cortical neurons (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Homodimer (By similarity). Interacts with GLMN. Associates with HSP90AA1 and HSP70 in steroid hormone receptor complexes. Also interacts with peroxisomal phytanoyl-CoA alpha-hydroxylase (PHYH). Interacts with NR3C1 and dynein. Interacts with HSF1 in the HSP90 complex. Associates with tubulin. Interacts with MAPT/TAU (By similarity). Interacts (via TPR domain) with S100A1, S100A2 and S100A6; the interaction is Ca(2+) dependent. Interaction with S100A1 and S100A2 (but not with S100A6) leads to inhibition of FKBP4-HSP90 interaction. Interacts with dynein; causes partially NR3C1 transport to the nucleus.

Family&Domains:

The PPIase activity is mainly due to the first PPIase FKBP-type domain (1-138 AA).

The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.

The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.

The TPR repeats mediate mitochondrial localization.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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