Phospho-CDC5L (Tyr232) Antibody - #AF7063

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Product: Phospho-CDC5L (Tyr232) Antibody
Catalog: AF7063
Description: Rabbit polyclonal antibody to Phospho-CDC5L (Tyr232)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 92kDa; 92kD(Calculated).
Uniprot: Q99459
RRID: AB_2843503

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(90%), Horse(100%), Sheep(90%), Rabbit(90%), Dog(100%), Chicken(90%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Phospho-CDC5L (Tyr232) Antibody detects endogenous levels of CDC5L only when phosphorylated at Tyr232.
RRID:
AB_2843503
Cite Format: Affinity Biosciences Cat# AF7063, RRID:AB_2843503.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC5; CDC5 cell division cycle 5-like (S. pombe); Cdc5 related protein; CDC5-LIKE; Cdc5-like protein; Cdc5l; CDC5L_HUMAN; CEF1; Cell division cycle 5 S. pombe homolog of; Cell division cycle 5-like protein; dJ319D22.1; KIAA0432; PCDC5RP; Pombe Cdc5 related protein; Pombe Cdc5-related protein;

Immunogens

Immunogen:
Uniprot:

Q99459(CDC5L_HUMAN) >>Visit HPA database.

Gene(ID):
CDC5L(988)
Expression:
Q99459 CDC5L_HUMAN:

Ubiquitously expressed in both fetal and adult tissues.

Sequence:
MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGAEGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
90
Sheep
90
Chicken
90
Rabbit
90
Xenopus
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99459 As Substrate

Site PTM Type Enzyme
K25 Ubiquitination
K59 Ubiquitination
K70 Ubiquitination
K165 Methylation
K166 Acetylation
K168 Acetylation
K187 Ubiquitination
Y210 Phosphorylation
K219 Sumoylation
K219 Ubiquitination
Y225 Phosphorylation
T227 Phosphorylation
S228 Phosphorylation
Y232 Phosphorylation
R252 Methylation
S253 Phosphorylation
S273 Phosphorylation
S277 Phosphorylation
S303 Phosphorylation
S317 Phosphorylation
T344 Phosphorylation
S347 Phosphorylation
T352 Phosphorylation
T355 Phosphorylation
S358 Phosphorylation
T373 Phosphorylation
T377 Phosphorylation
K380 Ubiquitination
T385 Phosphorylation
S390 Phosphorylation
S393 Phosphorylation
T396 Phosphorylation
T404 Phosphorylation
T407 Phosphorylation
S410 Phosphorylation P24941 (CDK2)
T411 Phosphorylation P24941 (CDK2)
T415 Phosphorylation
S417 Phosphorylation
T424 Phosphorylation
S427 Phosphorylation
T429 Phosphorylation
T430 Phosphorylation
K432 Ubiquitination
S437 Phosphorylation
T438 Phosphorylation
T442 Phosphorylation
K447 Ubiquitination
Y459 Phosphorylation
S460 Phosphorylation
S463 Phosphorylation
Y464 Phosphorylation
K466 Ubiquitination
K487 Ubiquitination
K500 Ubiquitination
T510 Phosphorylation
Y511 Phosphorylation
Y621 Phosphorylation
K623 Ubiquitination
K626 Acetylation
S691 Phosphorylation
K694 Ubiquitination
Y788 Phosphorylation
K795 Ubiquitination
T797 Phosphorylation

Research Backgrounds

Function:

DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).

PTMs:

Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.

Subcellular Location:

Nucleus. Nucleus speckle. Cytoplasm.
Note: May shuttle between cytoplasm and nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed in both fetal and adult tissues.

Subunit Structure:

Homodimer. Interacts with DAPK3 (By similarity). Component of the precatalytic, catalytic and postcatalytic spliceosome complexes. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its C-terminus) directly in the complex with PRPF19 and BCAS2. Interacts (via its C-terminus) directly with PRGL1 (via its WD40 repeat domain); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts with CTNNBL1. Interacts with PRPF19 (via N-terminus) (By similarity).

Family&Domains:

Belongs to the CEF1 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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