Phospho-FBP1 (Tyr265) Antibody - #AF7060

(1)  
Product: Phospho-FBP1 (Tyr265) Antibody
Catalog: AF7060
Description: Rabbit polyclonal antibody to Phospho-FBP1 (Tyr265)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 37kDa; 37kD(Calculated).
Uniprot: P09467
RRID: AB_2843500

View similar products>>

   Size Price Inventory
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(89%), Horse(89%), Rabbit(100%), Dog(100%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
Phospho-FBP1 (Tyr265) Antibody detects endogenous levels of FBP1 only when phosphorylated at Tyr265.
RRID:
AB_2843500
Cite Format: Affinity Biosciences Cat# AF7060, RRID:AB_2843500.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

6-bisphosphatase 1; 6-bisphosphate 1-phosphohydrolase 1; D fructose 1 6 bisphosphate 1 phosphohydrolase 1; D-fructose-1; EC 3.1.3.11; F16P1_HUMAN; FBP; FBP 1; FBP1; FBPase 1; Fructose 1 6 bisphosphatase 1; Fructose bisphosphatase 1; Fructose-1; Growth inhibiting protein 17; Liver fructose bisphosphatase;

Immunogens

Immunogen:
Uniprot:

P09467(F16P1_HUMAN) >>Visit HPA database.

Gene(ID):
FBP1(2203)
Expression:
P09467 F16P1_HUMAN:

Expressed in pancreatic islets.

Sequence:
MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
89
Horse
89
Bovine
89
Chicken
89
Sheep
78
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09467 As Substrate

Site PTM Type Enzyme
T28 Phosphorylation
Y58 Phosphorylation
S63 Phosphorylation
K72 Acetylation
S88 Phosphorylation
S144 Phosphorylation
Y216 Phosphorylation
Y227 Phosphorylation
K232 Acetylation
Y241 Phosphorylation
Y245 Phosphorylation
T256 Phosphorylation
Y259 Phosphorylation
Y265 Phosphorylation
K269 Acetylation
S271 Phosphorylation
T297 Phosphorylation
T298 Phosphorylation
S321 Phosphorylation
K330 Acetylation

Research Backgrounds

Function:

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.

Tissue Specificity:

Expressed in pancreatic islets.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the FBPase class 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.