Phospho-SLC32A1 (Ser462) Antibody | Affinity Biosciences

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Product:	Phospho-SLC32A1 (Ser462) Antibody
Catalog:	AF7051
Description:	Rabbit polyclonal antibody to Phospho-SLC32A1 (Ser462)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	57kDa; 57kD(Calculated).
Uniprot:	Q9H598
RRID:	AB_2843491

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-SLC32A1 (Ser462) Antibody detects endogenous levels of SLC32A1 only when phosphorylated at Ser462.

RRID:

AB_2843491
Cite Format: Affinity Biosciences Cat# AF7051, RRID:AB_2843491.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bA122O1.1; GABA and glycine transporter; hVIAAT; SLC32A 1; Slc32a1; solute carrier family 32 (GABA vesicular transporter) member 1; Solute carrier family 32 member 1; Vesicular GABA Amino Acid Transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; VGAT; VIAAT; VIAAT_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q9H598(VIAAT_HUMAN) >>Visit HPA database.

Gene(ID):

SLC32A1(140679)

Expression:

Q9H598 VIAAT_HUMAN:

Retina. Expressed throughout the horizontal cells or more specifically at the terminals.

Sequence:

Q9H598 VIAAT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATLLRSKLSNVATSVSNKSQAKMSGMFARMGFQAATDEEAVGFAHCDDLDFEHRQGLQMDILKAEGEPCGDEGAEAPVEGDIHYQRGSGAPLPPSGSKDQVGGGGEFGGHDKPKITAWEAGWNVTNAIQGMFVLGLPYAILHGGYLGLFLIIFAAVVCCYTGKILIACLYEENEDGEVVRVRDSYVAIANACCAPRFPTLGGRVVNVAQIIELVMTCILYVVVSGNLMYNSFPGLPVSQKSWSIIATAVLLPCAFLKNLKAVSKFSLLCTLAHFVINILVIAYCLSRARDWAWEKVKFYIDVKKFPISIGIIVFSYTSQIFLPSLEGNMQQPSEFHCMMNWTHIAACVLKGLFALVAYLTWADETKEVITDNLPGSIRAVVNIFLVAKALLSYPLPFFAAVEVLEKSLFQEGSRAFFPACYSGDGRLKSWGLTLRCALVVFTLLMAIYVPHFALLMGLTGSLTGAGLCFLLPSLFHLRLLWRKLLWHQVFFDVAIFVIGGICSVSGFVHSLEGLIEAYRTNAED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Rabbit

100

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H598 As Substrate

Q9H598

Site	PTM Type	Source
S17	Phosphorylation	Uniprot
S20	Phosphorylation	Uniprot
S96	Phosphorylation	Uniprot
S98	Phosphorylation	Uniprot
Y300	Phosphorylation	Uniprot
T443	Phosphorylation	Uniprot
S462	Phosphorylation	Uniprot

Research Backgrounds

Q9H598_VIAAT_HUMAN

Function:

Involved in the uptake of GABA and glycine into the synaptic vesicles.

Subcellular Location:

Cytoplasmic vesicle membrane>Multi-pass membrane protein.

Tissue Specificity:

Retina. Expressed throughout the horizontal cells or more specifically at the terminals.

Family&Domains:

Belongs to the amino acid/polyamine transporter 2 family.

Research Fields

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

· Organismal Systems > Nervous system > GABAergic synapse.

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For Research Use Only.
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Phospho-SLC32A1 (Ser462) Antibody - #AF7051