Phospho-SLC16A1 (Ser213) Antibody | Affinity Biosciences

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AF7044 at 1/100 staining human kidney tissue sections by IHC-P.

AF7044 at 1/100 staining rat kidney tissue sections by IHC-P.

AF7044 at 1/100 staining mouse kidney tissue sections by IHC-P.

peptide-ELISA analysis of AF7044.

Figure 6 The expression level of TF, p-TF, SLC16A1, and p-SLC16A1 was evaluated by the Western blot assay.

Product:	Phospho-SLC16A1 (Ser213) Antibody
Catalog:	AF7044
Description:	Rabbit polyclonal antibody to Phospho-SLC16A1 (Ser213)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	54kDa; 54kD(Calculated).
Uniprot:	P53985
RRID:	AB_2843484

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$350	In stock
200ul	$450	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-SLC16A1 (Ser213) Antibody detects endogenous levels of SLC16A1 only when phosphorylated at Ser213.

RRID:

AB_2843484
Cite Format: Affinity Biosciences Cat# AF7044, RRID:AB_2843484.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ36745; HHF7; MCT 1; MCT; MGC44475; Monocarboxylate transporter 1; Monocarboxylate transporter; Monocarboxylate transporter isoform 1; Monocarboxylic acid transporter 1; MOT1_HUMAN; Slc16a1; SLC16A1 protein; Solute carrier family 16 (monocarboxylic acid transporters) member 1; Solute carrier family 16 member 1 (monocarboxylic acid transporter 1); Solute carrier family 16 member 1;

Immunogens

Immunogen:

Uniprot:

P53985(MOT1_HUMAN) >>Visit HPA database.

Gene(ID):

SLC16A1(6566)

Expression:

P53985 MOT1_HUMAN:

Detected in heart and in blood lymphocytes and monocytes (at protein level). Widely expressed.

Sequence:

P53985 MOT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPPAVGGPVGYTPPDGGWGWAVVIGAFISIGFSYAFPKSITVFFKEIEGIFHATTSEVSWISSIMLAVMYGGGPISSILVNKYGSRIVMIVGGCLSGCGLIAASFCNTVQQLYVCIGVIGGLGLAFNLNPALTMIGKYFYKRRPLANGLAMAGSPVFLCTLAPLNQVFFGIFGWRGSFLILGGLLLNCCVAGALMRPIGPKPTKAGKDKSKASLEKAGKSGVKKDLHDANTDLIGRHPKQEKRSVFQTINQFLDLTLFTHRGFLLYLSGNVIMFFGLFAPLVFLSSYGKSQHYSSEKSAFLLSILAFVDMVARPSMGLVANTKPIRPRIQYFFAASVVANGVCHMLAPLSTTYVGFCVYAGFFGFAFGWLSSVLFETLMDLVGPQRFSSAVGLVTIVECCPVLLGPPLLGRLNDMYGDYKYTYWACGVVLIISGIYLFIGMGINYRLLAKEQKANEQKKESKEEETSIDVAGKPNEVTKAAESPDQKDTDGGPKEEESPV

PTMs - P53985 As Substrate

P53985

Site	PTM Type	Source
S33	Phosphorylation	Uniprot
Y34	Phosphorylation	Uniprot
K211	Ubiquitination	Uniprot
S213	Phosphorylation	Uniprot
K216	Ubiquitination	Uniprot
K224	Ubiquitination	Uniprot
K239	Ubiquitination	Uniprot
S244	Phosphorylation	Uniprot
T248	Phosphorylation	Uniprot
K459	Ubiquitination	Uniprot
S461	Phosphorylation	Uniprot
K462	Ubiquitination	Uniprot
T466	Phosphorylation	Uniprot
S467	Phosphorylation	Uniprot
K473	Ubiquitination	Uniprot
K479	Ubiquitination	Uniprot
S483	Phosphorylation	Uniprot
K487	Ubiquitination	Uniprot
T489	Phosphorylation	Uniprot
K494	Methylation	Uniprot
K494	Ubiquitination	Uniprot
S498	Phosphorylation	Uniprot

Research Backgrounds

P53985_MOT1_HUMAN

Function:

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Detected in heart and in blood lymphocytes and monocytes (at protein level). Widely expressed.

Subunit Structure:

Interacts with EMB. Interaction with either BSG or EMB is required for expression at the cell membrane (By similarity). Interacts with BSG; this is required for expression at the cell membrane.

Family&Domains:

Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

References

1). Effects of Asiaticoside Treatment on the Survival of Random Skin Flaps in Rats. JOURNAL OF INVESTIGATIVE SURGERY, 2019 (PubMed: 30898065) [IF=1.9]

2). Investigations on the Changes of Serum Proteins in Rabbits after Trimeresurus stejnegeri Venom Injection via Mass Spectrometry-Based Proteomics. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 35783526)

Application: WB Species: Rabbit Sample:

Figure 6 The expression level of TF, p-TF, SLC16A1, and p-SLC16A1 was evaluated by the Western blot assay. Compared to the control group, p-TF/TF was significantly upregulated in the model group. In addition, compared to control, the expression level of p-SLC16A1/SLC16A1 was found significantly suppressed in the model group (p < 0.05).

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Phospho-SLC16A1 (Ser213) Antibody - #AF7044