Phospho-KLF5 (Ser303) Antibody - #AF7042

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Product: Phospho-KLF5 (Ser303) Antibody
Catalog: AF7042
Description: Rabbit polyclonal antibody to Phospho-KLF5 (Ser303)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 51kDa; 51kD(Calculated).
Uniprot: Q13887
RRID: AB_2843482

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-KLF5 (Ser303) Antibody detects endogenous levels of KLF5 only when phosphorylated at Ser303.
RRID:
AB_2843482
Cite Format: Affinity Biosciences Cat# AF7042, RRID:AB_2843482.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Basic transcription element binding protein 2; Basic transcription element-binding protein 2; BTE binding protein 2; BTE-binding protein 2; BTEB 2; BTEB2; CKLF; Colon krueppel like factor; Colon krueppel-like factor; GC box binding protein 2; GC-box-binding protein 2; IKLF; Intestinal enriched krueppel like factor; Intestinal-enriched krueppel-like factor; KLF 5; Klf5; KLF5_HUMAN; Krueppel like factor 5; Krueppel-like factor 5; Kruppel like factor 5 intestinal; Transcription factor BTEB 2; Transcription factor BTEB2;

Immunogens

Immunogen:
Uniprot:

Q13887(KLF5_HUMAN) >>Visit HPA database.

Gene(ID):
KLF5(688)
Expression:
Q13887 KLF5_HUMAN:

Expressed only in testis and placenta.

Sequence:
MATRVLSMSARLGPVPQPPAPQDEPVFAQLKPVLGAANPARDAALFPGEELKHAHHRPQAQPAPAQAPQPAQPPATGPRLPPEDLVQTRCEMEKYLTPQLPPVPIIPEHKKYRRDSASVVDQFFTDTEGLPYSINMNVFLPDITHLRTGLYKSQRPCVTHIKTEPVAIFSHQSETTAPPPAPTQALPEFTSIFSSHQTAAPEVNNIFIKQELPTPDLHLSVPTQQGHLYQLLNTPDLDMPSSTNQTAAMDTLNVSMSAAMAGLNTHTSAVPQTAVKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVNSQNIQPVRYNRRSNPDLEKRRIHYCDYPGCTKVYTKSSHLKAHLRTHTGEKPYKCTWEGCDWRFARSDELTRHYRKHTGAKPFQCGVCNRSFSRSDHLALHMKRHQN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13887 As Substrate

Site PTM Type Enzyme
K31 Ubiquitination
K110 Sumoylation
K110 Ubiquitination
S116 Phosphorylation
S153 Phosphorylation Q05655 (PRKCD)
K162 Sumoylation
K209 Sumoylation
S303 Phosphorylation P49841 (GSK3B)
T323 Phosphorylation
T346 Phosphorylation
Y359 Phosphorylation
S363 Phosphorylation
K369 Acetylation
Y377 Phosphorylation
K391 Sumoylation
K391 Ubiquitination
T396 Phosphorylation
T398 Phosphorylation
K401 Ubiquitination
S441 Phosphorylation
S443 Phosphorylation

Research Backgrounds

Function:

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

PTMs:

Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed only in testis and placenta.

Subunit Structure:

Interacts with WWP1.

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the krueppel C2H2-type zinc-finger protein family.

References

1). Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop. Cell death & disease, 2022 (PubMed: 36042208) [IF=9.0]

Application: WB    Species: Human    Sample: MDA-MB-231 cells

Fig. 4 DCC-2036 and AXL regulate the KLF5 protein level through Akt/GSK3β pathway. A DCC-2036 decreased the phosphorylation of AKT (Ser473), GSK3β (Ser9), and total KLF5, and increased the phosphorylation of KLF5 (Ser303). TNBC cells were treated with DCC-2036 for 48 h, followed by the WB assay. B Gas6 promoted the increase of p-AKT, p-GSK3β, and total KLF5, as well as the decrease of p-KLF5 in MDA-MB-231 cells and HS-578T cells. Cells were treated with Gas6 (200 ng/ml) for 0, 30, 60, 90, and 120 min, and then examined by WB.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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