Phospho-GDF9 (Tyr394) Antibody | Affinity Biosciences

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Product:	Phospho-GDF9 (Tyr394) Antibody
Catalog:	AF7035
Description:	Rabbit polyclonal antibody to Phospho-GDF9 (Tyr394)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	51kDa; 51kD(Calculated).
Uniprot:	O60383
RRID:	AB_2843475

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(90%)

Clonality:

Polyclonal

Specificity:

Phospho-GDF9 (Tyr394) Antibody detects endogenous levels of GDF9 only when phosphorylated at Tyr394.

RRID:

AB_2843475
Cite Format: Affinity Biosciences Cat# AF7035, RRID:AB_2843475.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GDF9; Growth differentiation factor 9;

Immunogens

Immunogen:

Uniprot:

O60383(GDF9_HUMAN) >>Visit HPA database.

Gene(ID):

GDF9(2661)

Expression:

O60383 GDF9_HUMAN:

Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level).

Sequence:

O60383 GDF9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MARPNKFLLWFCCFAWLCFPISLGSQASGGEAQIAASAELESGAMPWSLLQHIDERDRAGLLPALFKVLSVGRGGSPRLQPDSRALHYMKKLYKTYATKEGIPKSNRSHLYNTVRLFTPCTRHKQAPGDQVTGILPSVELLFNLDRITTVEHLLKSVLLYNINNSVSFSSAVKCVCNLMIKEPKSSSRTLGRAPYSFTFNSQFEFGKKHKWIQIDVTSLLQPLVASNKRSIHMSINFTCMKDQLEHPSAQNGLFNMTLVSPSLILYLNDTSAQAYHSWYSLHYKRRPSQGPDQERSLSAYPVGEEAAEDGRSSHHRHRRGQETVSSELKKPLGPASFNLSEYFRQFLLPQNECELHDFRLSFSQLKWDNWIVAPHRYNPRYCKGDCPRAVGHRYGSPVHTMVQNIIYEKLDSSVPRPSCVPAKYSPLSVLTIEPDGSIAYKEYEDMIATKCTCR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Horse

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60383 As Substrate

O60383

Site	PTM Type	Source
S156	Phosphorylation	Uniprot
S170	Phosphorylation	Uniprot
S185	Phosphorylation	Uniprot
S186	Phosphorylation	Uniprot
T189	Phosphorylation	Uniprot
S196	Phosphorylation	Uniprot
S288	Phosphorylation	Uniprot
S325	Phosphorylation	Uniprot
Y394	Phosphorylation	Uniprot
Y407	Phosphorylation	Uniprot
Y440	Phosphorylation	Uniprot

Research Backgrounds

O60383_GDF9_HUMAN

Function:

Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.

PTMs:

Phosphorylated; phosphorylation is critical for GDF9 function. In vitro, can be phosphorylated by CK at Ser-325.

Subcellular Location:

Secreted.

Tissue Specificity:

Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level).

Subunit Structure:

Homodimer or heterodimer (Potential). But, in contrast to other members of this family, cannot be disulfide-linked (By similarity).

Family&Domains:

Belongs to the TGF-beta family.

Research Fields

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

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Phospho-GDF9 (Tyr394) Antibody - #AF7035