Phospho-N-WASP (Tyr256) Antibody - #AF7032

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Product: Phospho-N-WASP (Tyr256) Antibody
Catalog: AF7032
Description: Rabbit polyclonal antibody to Phospho-N-WASP (Tyr256)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 54kDa; 55kD(Calculated).
Uniprot: O00401
RRID: AB_2843472

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-N-WASP (Tyr256) Antibody detects endogenous levels of N-WASP only when phosphorylated at Tyr256.
RRID:
AB_2843472
Cite Format: Affinity Biosciences Cat# AF7032, RRID:AB_2843472.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp779G0847; MGC48327; N-WASP; Neural Wiskott Aldrich syndrome protein; Neural Wiskott-Aldrich syndrome protein; NWASP; Wasl; WASL_HUMAN; WASPB; Wiskott Aldrich syndrome gene like; Wiskott Aldrich syndrome gene like protein; Wiskott Aldrich syndrome like; WiskottAldrich syndrome like;

Immunogens

Immunogen:
Uniprot:

O00401(WASL_HUMAN) >>Visit HPA database.

Gene(ID):
WASL(8976)
Sequence:
MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDEEDFEDDDEWED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00401 As Substrate

Site PTM Type Enzyme
S2 Acetylation
T23 Phosphorylation
S58 Phosphorylation
Y95 Phosphorylation
Y100 Phosphorylation
S102 Phosphorylation
T135 Phosphorylation
Y175 Phosphorylation P00519 (ABL1)
S183 Phosphorylation
K186 Ubiquitination
T199 Phosphorylation Q13627 (DYRK1A)
T205 Phosphorylation Q13627 (DYRK1A)
S242 Phosphorylation
K253 Ubiquitination
Y256 Phosphorylation P42684 (ABL2) , P00519 (ABL1) , Q05397 (PTK2)
K261 Ubiquitination
T262 Phosphorylation Q13627 (DYRK1A)
R274 Methylation
R284 Methylation
R305 Methylation
R307 Methylation
R329 Methylation
S426 Phosphorylation
S430 Phosphorylation
S456 Phosphorylation
T457 Phosphorylation
T460 Phosphorylation
T464 Phosphorylation
S484 Phosphorylation
S485 Phosphorylation

Research Backgrounds

Function:

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity). Plays a role in dendrite spine morphogenesis (By similarity). Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear.

PTMs:

Phosphorylation at Ser-242, Tyr-256, Ser-484 and Ser-485 enhances actin polymerization activity.

Subcellular Location:

Cytoplasm>Cytoskeleton. Nucleus. Cytoplasm.
Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated (By similarity). Exported from the nucleus by an nuclear export signal (NES)-dependent mechanism to the cytoplasm (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds actin and the Arp2/3 complex. Interacts with CDC42. Interacts with FCHSD1 (By similarity). Interacts with FCHSD2. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9 (By similarity). Interacts with the WW domains of PRPF40A/FBP11 (By similarity). Interacts with PTK2/FAK1 (By similarity). Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Interacts with NOSTRIN. Binds to TNK2. Interacts with SNX33. Interacts with NONO (via second RRM domain); the interaction is direct. Component of a multiprotein complex with NONO and SFPQ; associates with the complex via direct interaction with NONO.

(Microbial infection) Interacts with E.coli effector protein EspF(U). Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF(U).

(Microbial infection) Interacts with Shigella flexneri protein IcsA. The interaction with IcsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

References

1). Novel long noncoding RNA LINC02820 augments TNF signaling pathway to remodel cytoskeleton and potentiate metastasis in esophageal squamous cell carcinoma. Cancer Gene Therapy, 2022 (PubMed: 36357564) [IF=6.4]

Application: WB    Species: Mouse    Sample:

Fig. 3 LINC02820 promotes metastasis in ESCC via cytoskeletal remodeling, not EMT. A Analysis of the migration and invasion ability of K180 cells transfected with SiLINC02820 or siNC (scale bar, 100 μm). B Analysis of the migration and invasion ability of K30 cells transfected with OE- LINC02820 or Vector (scale bar, 100 μm). C The migration and invasion ability of K180 cells transfected with LINC02820 suppression plasmid (sg-3 and sg-4) or control plasmid (Guide) (scale bar, 100 μm). D, E K30 Vector and K30 OE-LINC02820 cell lines were injected in mice footpads and the popliteal lymph node of nude mice was shown, and the metastasis of the popliteal lymph node. F The protein levels of EMT markers when LINC02820 are inhibited or increased. G The levels of N-WASP, p-N-WASP, and Cortactin about invasive pseudopodia. H Inhibition of LINC02820 reduced the F-actin in K180 and K410 cells (scale bar, 10 μm). I Overexpression of LINC02820 increased the F-actin in K30 and EC109 cells (scale bar, 10 μm).

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