PRPS1 Antibody - #AF7030

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Product: PRPS1 Antibody
Catalog: AF7030
Description: Rabbit polyclonal antibody to PRPS1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 34 KD; 35kD(Calculated).
Uniprot: P60891
RRID: AB_2843470

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Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(78%), Bovine(78%), Horse(78%), Sheep(78%), Rabbit(63%), Dog(0%), Chicken(67%), Xenopus(57%)
Clonality:
Polyclonal
Specificity:
PRPS1 Antibody detects endogenous levels of total PRPS1.
RRID:
AB_2843470
Cite Format: Affinity Biosciences Cat# AF7030, RRID:AB_2843470.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARTS; CMTX5; Deafness 2 perceptive congenital; Deafness X linked 2 perceptive congenital; DFN2; DFNX1; EC 2.7.6.1; KIAA0967; Phosphoribosyl pyrophosphate synthase I; Phosphoribosyl pyrophosphate synthetase I; PPRibP; Prps1; PRPS1_HUMAN; PRS I; PRS-I; PRSI; Ribose phosphate pyrophosphokinase I; Ribose-phosphate pyrophosphokinase 1;

Immunogens

Immunogen:
Uniprot:

P60891(PRPS1_HUMAN) >>Visit HPA database.

Gene(ID):
PRPS1(5631)
Description:
Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.
Sequence:
MPNIKIFSGSSHQDLSQKIADRLGLELGKVVTKKFSNQETCVEIGESVRGEDVYIVQSGCGEINDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKDKSRAPISAKLVANMLSVAGADHIITMDLHASQIQGFFDIPVDNLYAEPAVLKWIRENISEWRNCTIVSPDAGGAKRVTSIADRLNVDFALIHKERKKANEVDRMVLVGDVKDRVAILVDDMADTCGTICHAADKLLSAGATRVYAILTHGIFSGPAISRINNACFEAVVVTNTIPQEDKMKHCSKIQVIDISMILAEAIRRTHNGESVSYLFSHVPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
78
Horse
78
Bovine
78
Sheep
78
Chicken
67
Rabbit
63
Xenopus
57
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P60891 As Substrate

Site PTM Type Enzyme
K5 Ubiquitination
K18 Ubiquitination
R22 Methylation
K29 Ubiquitination
S36 Phosphorylation
S103 Phosphorylation
Y146 Phosphorylation
R156 Methylation
K176 Acetylation
K176 Ubiquitination
S180 Phosphorylation
R184 Methylation
K194 Acetylation
K194 Ubiquitination
R204 Methylation
K212 Acetylation
K212 Ubiquitination
T225 Phosphorylation
T228 Phosphorylation
K235 Ubiquitination
S238 Phosphorylation
T242 Phosphorylation
Y245 Phosphorylation
K280 Ubiquitination
S308 Phosphorylation
S310 Phosphorylation
Y311 Phosphorylation

Research Backgrounds

Function:

Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.

Subunit Structure:

Homodimer. The active form is probably a hexamer composed of 3 homodimers.

Family&Domains:

Belongs to the ribose-phosphate pyrophosphokinase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

 

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For Research Use Only.
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