Product: ketohexokinase Antibody
Catalog: AF7029
Description: Rabbit polyclonal antibody to ketohexokinase
Application: WB
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep
Mol.Wt.: 33 KD; 33kD(Calculated).
Uniprot: P50053
RRID: AB_2843469

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(88%), Horse(100%), Sheep(88%)
Clonality:
Polyclonal
Specificity:
ketohexokinase Antibody detects endogenous levels of total ketohexokinase.
RRID:
AB_2843469
Cite Format: Affinity Biosciences Cat# AF7029, RRID:AB_2843469.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.7.1.3; Fructokinase; Hepatic fructokinase; Ketohexokinase; Ketohexokinase isoform a; KHK; KHK_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human ketohexokinase, corresponding to a region within N-terminal amino acids.

Uniprot:
Gene(ID):
Expression:
P50053 KHK_HUMAN:

Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.

Description:
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Sequence:
MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSMAPGHVADFLVADFRRRGVDVSQVAWQSKGDTPSSCCIINNSNGNRTIVLHDTSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
88
Sheep
88
Pig
63
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50053 As Substrate

Site PTM Type Enzyme
S90 Phosphorylation
S116 Phosphorylation
K134 Ubiquitination
K148 Ubiquitination
K197 Ubiquitination

Research Backgrounds

Function:

Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.

Tissue Specificity:

Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the carbohydrate kinase PfkB family.

Research Fields

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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