Phospho-ketohexokinase (Ser80) Antibody-Isoform A

WB
pElisa

1/2

Western blot analysis of extracts from mouse kidney, using Phospho-ketohexokinase(S81) Antibody.

Product:	Phospho-ketohexokinase (Ser80) Antibody-Isoform A
Catalog:	AF8612
Description:	Rabbit polyclonal antibody to Phospho-ketohexokinase (Ser80)-Isoform A
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep
Mol.Wt.:	33 KD; 33kD(Calculated).
Uniprot:	P50053
RRID:	AB_2843461

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(88%), Horse(100%), Sheep(88%)

Clonality:

Polyclonal

Specificity:

Phospho-ketohexokinase (Ser80) Antibody-Isoform A detects endogenous levels of ketohexokinase only when phosphorylated at S80.

RRID:

AB_2843461
Cite Format: Affinity Biosciences Cat# AF8612, RRID:AB_2843461.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EC 2.7.1.3; Fructokinase; Hepatic fructokinase; Ketohexokinase; Ketohexokinase isoform a; KHK; KHK_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human Ketohexokinase(Isoform A) around the phosphorylation site of S80.

Uniprot:

P50053(KHK_HUMAN) >>Visit HPA database.

Gene(ID):

KHK(3795)

Expression:

P50053 KHK_HUMAN:

Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.

Description:

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Sequence:

P50053 KHK_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTVLSLLGAPCAFMGSMAPGHVADFLVADFRRRGVDVSQVAWQSKGDTPSSCCIINNSNGNRTIVLHDTSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

Sheep

Pig

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50053 As Substrate

P50053

Site	PTM Type	Source
S90	Phosphorylation	Uniprot
S116	Phosphorylation	Uniprot
K134	Ubiquitination	Uniprot
K148	Ubiquitination	Uniprot
K197	Ubiquitination	Uniprot

Research Backgrounds

P50053_KHK_HUMAN

Function:

Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.

Tissue Specificity:

Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the carbohydrate kinase PfkB family.

Research Fields

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-ketohexokinase (Ser80) Antibody-Isoform A - #AF8612