Product: INCENP Antibody
Catalog: AF7026
Description: Rabbit polyclonal antibody to INCENP
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 105.4kDa; 105kD(Calculated).
Uniprot: Q9NQS7
RRID: AB_2843450

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
INCENP Antibody detects endogenous levels of total INCENP.
RRID:
AB_2843450
Cite Format: Affinity Biosciences Cat# AF7026, RRID:AB_2843450.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

binds and activates aurora B and C in vivo and in vitro; Chromosomal passenger protein; INCE_HUMAN; INCENP; Inner centromere protein; Inner centromere protein antigens 135/155kDa; Inner centromere protein antigens 135kD 155kD; Inner centromere protein INCENP;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus asssociated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules (PubMed:15316025, PubMed:12925766, PubMed:27332895). The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity). Activates AURKB and AURKC (PubMed:27332895). Required for localization of CBX5 to mitotic centromeres (PubMed:21346195). Controls the kinetochore localization of BUB1 (PubMed:16760428).
Sequence:
MGTTAPGPIHLLELCDQKLMEFLCNMDNKDLVWLEEIQEEAERMFTREFSKEPELMPKTPSQKNRRKKRRISYVQDENRDPIRRRLSRRKSRSSQLSSRRLRSKDSVEKLATVVGENGSVLRRVTRAAAAAAAATMALAAPSSPTPESPTMLTKKPEDNHTQCQLVPVVEIGISERQNAEQHVTQLMSTEPLPRTLSPTPASATAPTSQGIPTSDEESTPKKSKARILESITVSSLMATPQDPKGQGVGTGRSASKLRIAQVSPGPRDSPAFPDSPWRERVLAPILPDNFSTPTGSRTDSQSVRHSPIAPSSPSPQVLAQKYSLVAKQESVVRRASRRLAKKTAEEPAASGRIICHSYLERLLNVEVPQKVGSEQKEPPEEAEPVAAAEPEVPENNGNNSWPHNDTEIANSTPNPKPAASSPETPSAGQQEAKTDQADGPREPPQSARRKRSYKQAVSELDEEQHLEDEELQPPRSKTPSSPCPASKVVRPLRTFLHTVQRNQMLMTPTSAPRSVMKSFIKRNTPLRMDPKCSFVEKERQRLENLRRKEEAEQLRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEEVEARRKQEEEARRLRWLQQEEEERRHQELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERREQERREQERREQERQLAEQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAERQLQEEQEKKAKEAAGASKALNVTVDVQSPACTSYQMTPQGHRAPPKINPDNYGMDLNSDDSTDDEAHPRKPIPTWARGTPLSQAIIHQYYHPPNLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSPPLQGARVPSSLAYSLKKH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
88
Bovine
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NQS7 As Substrate

Site PTM Type Enzyme
S50 Phosphorylation
K51 Ubiquitination
T59 Phosphorylation P06493 (CDK1) , Q96GD4 (AURKB)
S72 Phosphorylation Q96GD4 (AURKB)
Y73 Phosphorylation
S91 Phosphorylation
S93 Phosphorylation
S94 Phosphorylation
S103 Phosphorylation
S106 Phosphorylation
K109 Ubiquitination
T112 Phosphorylation
S119 Phosphorylation
T135 Phosphorylation
S142 Phosphorylation
S143 Phosphorylation
T145 Phosphorylation
S148 Phosphorylation
T150 Phosphorylation
T153 Phosphorylation
T195 Phosphorylation
S197 Phosphorylation
T199 Phosphorylation
S202 Phosphorylation
T204 Phosphorylation
T207 Phosphorylation
S208 Phosphorylation
T213 Phosphorylation
S214 Phosphorylation
S218 Phosphorylation
T219 Phosphorylation
S223 Phosphorylation
S230 Phosphorylation
T232 Phosphorylation
S234 Phosphorylation
S235 Phosphorylation
T239 Phosphorylation
T250 Phosphorylation
S253 Phosphorylation
S255 Phosphorylation
S263 Phosphorylation
S269 Phosphorylation
S275 Phosphorylation
S291 Phosphorylation
T292 Phosphorylation
T294 Phosphorylation
S296 Phosphorylation
T298 Phosphorylation
S300 Phosphorylation
S302 Phosphorylation
S306 Phosphorylation
S311 Phosphorylation
S312 Phosphorylation
S314 Phosphorylation
K321 Ubiquitination
Y322 Phosphorylation
S323 Phosphorylation
K327 Sumoylation
K327 Ubiquitination
S330 Phosphorylation
S336 Phosphorylation
K341 Methylation
T343 Phosphorylation
S350 Phosphorylation
S357 Phosphorylation
K370 Ubiquitination
S373 Phosphorylation
S400 Phosphorylation
T406 Phosphorylation
S411 Phosphorylation
T412 Phosphorylation P06493 (CDK1)
S420 Phosphorylation
S421 Phosphorylation
T424 Phosphorylation
S446 Phosphorylation
S452 Phosphorylation
S458 Phosphorylation
S476 Phosphorylation
T478 Phosphorylation
S480 Phosphorylation
S481 Phosphorylation
S486 Phosphorylation
K487 Acetylation
T494 Phosphorylation Q96GD4 (AURKB)
T498 Phosphorylation
T507 Phosphorylation
T509 Phosphorylation
S510 Phosphorylation
S514 Phosphorylation
S518 Phosphorylation Q96GD4 (AURKB)
T524 Phosphorylation
K548 Ubiquitination
K602 Acetylation
K602 Ubiquitination
K609 Acetylation
K630 Acetylation
K630 Methylation
K631 Acetylation
K631 Methylation
K748 Ubiquitination
T793 Phosphorylation
S798 Phosphorylation
T802 Phosphorylation
S803 Phosphorylation
Y804 Phosphorylation
T807 Phosphorylation
Y822 Phosphorylation
S828 Phosphorylation
S831 Phosphorylation
T832 Phosphorylation
T844 Phosphorylation
T892 Phosphorylation Q96GD4 (AURKB) , PR:Q96GD4 (hAURKB)
S893 Phosphorylation Q96GD4 (AURKB) , PR:Q96GD4 (hAURKB)
S894 Phosphorylation PR:Q96GD4 (hAURKB) , Q9NRM7 (LATS2) , O95835 (LATS1) , Q96GD4 (AURKB)
S899 Phosphorylation Q96GD4 (AURKB)
S909 Phosphorylation
S910 Phosphorylation
Y913 Phosphorylation
S914 Phosphorylation
K916 Methylation
K917 Methylation

Research Backgrounds

Function:

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules. The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity). Activates AURKB and AURKC. Required for localization of CBX5 to mitotic centromeres. Controls the kinetochore localization of BUB1.

PTMs:

Phosphorylation by AURKB or AURKC at its C-terminal part is important for AURKB or AURKC activation by INCENP.

Subcellular Location:

Nucleus. Chromosome>Centromere. Cytoplasm>Cytoskeleton>Spindle. Midbody. Chromosome>Centromere>Kinetochore.
Note: Colocalized at synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocenters. Colocalizes with AURKB at a connecting strand traversing the centromere region and joining sister kinetochores, in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone (By similarity). Colocalizes with AURKB at mitotic chromosomes (PubMed:11453556). Localizes to inner kinetochore (PubMed:16760428). Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis (PubMed:15316025). Cocalizes to the equatorial cell cortex at anaphase (PubMed:11453556).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; in the complex binds directly to AURKB or AURKC via the IN box, and forms a triple-helix bundle-based subcomplex with BIRC5 and CDCA8 via its N-terminus. The reported homodimerization is questioned as the SAH domain is shown to be monomeric (By similarity). Interacts with H2AZ1 (By similarity). Interacts with CBX1 and CBX3. Interacts with tubulin beta chain. Interacts with EVI5. Interacts with CBX5; POGZ and INCENP compete for interaction with CBX5; regulates INCENP (and probably CPC) localization to centromeres in interphase and not required for proper mitotic progression or sister chromatid cohesion. Interacts with POGZ. Interacts with JTB.

Family&Domains:

The IN box mediates interaction with AURKB and AURKC.

The SAH (single alpha-helix) region is characterized by a high content of charged residues which are predicted to stabilize the alpha-helical structure by ionic bonds. It can refold after extension suggesting an in vivo force-dependent function. The isolated SAH domain is monomeric.

Belongs to the INCENP family.

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