Flag-Tag Antibody | Affinity Biosciences

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Western blot analysis of Flag-tag fusion protein, using Flag-Tag Antibody.

Product:	Flag-Tag Antibody
Catalog:	T0053
Description:	Rabbit polyclonal antibody to Flag-Tag
Application:	WB IF/ICC IP
Reactivity:	All
Uniprot:
RRID:	AB_2843447

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$150	In stock
100ul	$250	In stock
200ul	$350	In stock
1ml	$1200	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-1:30000, IF/ICC: 1:200, IP 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

All

Clonality:

Polyclonal

Specificity:

The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.

RRID:

AB_2843447
Cite Format: Affinity Biosciences Cat# T0053, RRID:AB_2843447.

Conjugate:

Unconjugated.

Purification:

Affinity-chromatography.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG tag antibody

Immunogens

Immunogen:

A synthetic peptide DYKDDDDK coupled to KLH.

Description:

FLAG tag polyclonal antibody is part of the series of Tag antibodies, the excellent quality in the research. FLAG tag antibody is a highly sensitive and affinity PAB applicable to FLAG-tagged fusion protein detection. FLAG tag antibody can detect FLAG tags in internal, C-terminal, or N-terminal recombinant proteins.

References

1). A neuroprotective role of Ufmylation through Atg9 in the aging brain of Drosophila. CELLULAR AND MOLECULAR LIFE SCIENCES, 2023 (PubMed: 37086384) [IF=8.0]

2). Natural isoflavone formononetin inhibits IgE-mediated mast cell activation and allergic inflammation by increasing IgE receptor degradation. Food & Function, 2023 (PubMed: 36880662) [IF=6.1]

3). Intergenic CircRNA Circ_0007379 Inhibits Colorectal Cancer Progression by Modulating miR-320a Biogenesis in a KSRP-Dependent Manner. International Journal of Biological Sciences, 2023

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Flag-Tag Antibody - #T0053