SLC1A5 Antibody - #AF6610

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Product: SLC1A5 Antibody
Catalog: AF6610
Description: Rabbit polyclonal antibody to SLC1A5
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 56 kDa; 57kD(Calculated).
Uniprot: Q15758
RRID: AB_2843431

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
SLC1A5 Antibody detects endogenous levels of total SLC1A5.
RRID:
AB_2843431
Cite Format: Affinity Biosciences Cat# AF6610, RRID:AB_2843431.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ASCT2; AAAT; AAAT_HUMAN; ATB(0); ATBO; Baboon M7 virus receptor; FLJ31068; M7V1; M7VS1; Neutral amino acid transporter B(0); R16; RD114/simian type D retrovirus receptor; RDR; RDRC; SLC1A5; Sodium dependent neutral amino acid transporter type 2; Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 (neutral amino acid transporter), member 5; Solute carrier family 1 member 5;

Immunogens

Immunogen:
Uniprot:

Q15758(AAAT_HUMAN) >>Visit HPA database.

Gene(ID):
SLC1A5(6510)
Expression:
Q15758 AAAT_HUMAN:

Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.

Description:
Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. May also be activated by insulin. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904). Acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses (PubMed:10051606, PubMed:10196349).
Sequence:
MVADPPRDSKGLAAAEPTANGGLALASIEDQGAAAGGYCGSRDQVRRCLRANLLVLLTVVAVVAGVALGLGVSGAGGALALGPERLSAFVFPGELLLRLLRMIILPLVVCSLIGGAASLDPGALGRLGAWALLFFLVTTLLASALGVGLALALQPGAASAAINASVGAAGSAENAPSKEVLDSFLDLARNIFPSNLVSAAFRSYSTTYEERNITGTRVKVPVGQEVEGMNILGLVVFAIVFGVALRKLGPEGELLIRFFNSFNEATMVLVSWIMWYAPVGIMFLVAGKIVEMEDVGLLFARLGKYILCCLLGHAIHGLLVLPLIYFLFTRKNPYRFLWGIVTPLATAFGTSSSSATLPLMMKCVEENNGVAKHISRFILPIGATVNMDGAALFQCVAAVFIAQLSQQSLDFVKIITILVTATASSVGAAGIPAGGVLTLAIILEAVNLPVDHISLILAVDWLVDRSCTVLNVEGDALGAGLLQNYVDRTESRSTEPELIQVKSELPLDPLPVPTEEGNPLLKHYRGPAGDATVASEKESVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
89
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15758 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S9 Phosphorylation
K10 Ubiquitination
S27 Phosphorylation
Y38 Phosphorylation
K178 Ubiquitination
S183 Phosphorylation
S194 Phosphorylation
S198 Phosphorylation
N212 N-Glycosylation
K247 Ubiquitination
K372 Ubiquitination
S493 Phosphorylation
T494 Phosphorylation
K502 Ubiquitination
S503 Phosphorylation
K522 Sumoylation
K522 Ubiquitination
Y524 Phosphorylation
R525 Methylation
T532 Phosphorylation
S535 Phosphorylation
K537 Acetylation
K537 Ubiquitination
S539 Phosphorylation

Research Backgrounds

Function:

Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids. Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development.

(Microbial infection) Acts as a cell surface receptor for Feline endogenous virus RD114.

(Microbial infection) Acts as a cell surface receptor for Baboon M7 endogenous virus.

(Microbial infection) Acts as a cell surface receptor for type D simian retroviruses.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.

Subunit Structure:

Homotrimer (Probable). Interacts with ERVH48-1/suppressyn; may negatively regulate syncytialization.

Family&Domains:

Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A5 subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Digestive system > Protein digestion and absorption.

References

1). Tripartite motif containing 33 demonstrated anticancer effect by degrading c‑Myc: Limitation of glutamine metabolism and proliferation in endometrial carcinoma cells. International journal of oncology, 2023 (PubMed: 37859625) [IF=5.2]

Application: WB    Species: Human    Sample: EC cell

Figure 4 TRIM33 overexpression inhibits the glutamine metabolism in HEC-1-A and AN3CA cells in vitro. (A) Glutamine uptake and (B) intracellular glutamate production of TRIM33-silenced or -overexpressed EC cells were analyzed using the commercial kits. (C and D) Reverse transcription-quantitative PCR and western blotting were used to detect the expression of SLC1A5 and GLS in EC cells. *P
2). Babaodan overcomes cisplatin resistance in cholangiocarcinoma via inhibiting YAP1. Pharmaceutical biology, 2024 (PubMed: 38571483) [IF=3.8]

Application: WB    Species: Human    Sample: CCAs

Figure 6. The extent of apoptosis, glutathione (GSH) synthesis, and the expression of DNA damage-related proteins were assessed in cholangiocarcinoma cells (CCAs) subjected to YAP1 knockdown or YAP1 overexpression. Western blot was used to measure protein levels (n = 3). With cisplatin incubation, YAP1 knockdown and 1 mg/mL babaodan (BBD) treatment decreased the (a) Bcl-2 level and (b) increased bax level, while the change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In the CCAs dealing with cisplatin, the expression levels of (d) p-YAP1/YAP1, (e) ATF4, and (f) SLC1A5 were decreased by YAP1 knockdown and BBD treatment. Additionally, the (g) γH2Ax level was increased and (h) the ERCC1 level was inhibited by YAP1 knockdown and BBD treatment. YAP1 overexpression antagonized the effect of BBD on these proteins. Representative protein bands are shown in (i), (j), and (k). (mean ± standard deviation) +p 
3). Dysfunction of Cytotoxic T Lymphocyte Induced by Hepatoma Cells through the Gln-GLS2-Endoplasmic Reticulum Stress Pathway. Frontiers in Bioscience-Landmark, 2022 (PubMed: 36042180)

Application: WB    Species: Mouse    Sample: cytotoxic T lymphocytes (CTLs) and hepatoma cells

Fig. 4. Glutamine (Gln) utilization in cytotoxic T lymphocytes (CTLs) and hepatoma cells. (A) Western blots were used to detect the protein expression levels of GLS1, GLS2, and SLC1A5 in the different groups. (B) After Gln deprivation, protein expression levels of GLS1, GLS2, and SLC1A5 in both CTLs and hepatoma cells were assessed by western blots.

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