V-ATPase H Antibody | Affinity Biosciences

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Western blot analysis of extracts from HUVEC cells, using V-ATPase H Antibody.

Product:	V-ATPase H Antibody
Catalog:	AF9227
Description:	Rabbit polyclonal antibody to V-ATPase H
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	55kDa; 56kD(Calculated).
Uniprot:	Q9UI12
RRID:	AB_2843417

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

V-ATPase H Antibody detects endogenous levels of total V-ATPase H.

RRID:

AB_2843417
Cite Format: Affinity Biosciences Cat# AF9227, RRID:AB_2843417.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Atp6v1h; ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H; CGI 11; CGI11; EC 3.6.3.14; MSTP042; NBP1; Nef binding protein 1; Nef-binding protein 1; Protein VMA13 homolog; SFD; SFDalpha; SFDbeta; V ATPase subunit H; V-ATPase 50/57 kDa subunits; V-ATPase subunit H; V-type proton ATPase subunit H; Vacuolar ATP synthase subunit H; Vacuolar proton pump subunit H; Vacuolar proton pump subunit SFD; VATH_HUMAN; VMA13;

Immunogens

Immunogen:

Uniprot:

Q9UI12(VATH_HUMAN) >>Visit HPA database.

Gene(ID):

ATP6V1H(51606)

Expression:

Q9UI12 VATH_HUMAN:

Widely expressed.

Sequence:

Q9UI12 VATH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTKMDIRGAVDAAVPTNIIAAKAAEVRANKVNWQSYLQGQMISAEDCEFIQRFEMKRSPEEKQEMLQTEGSQCAKTFINLMTHICKEQTVQYILTMVDDMLQENHQRVSIFFDYARCSKNTAWPYFLPMLNRQDPFTVHMAARIIAKLAAWGKELMEGSDLNYYFNWIKTQLSSQKLRGSGVAVETGTVSSSDSSQYVQCVAGCLQLMLRVNEYRFAWVEADGVNCIMGVLSNKCGFQLQYQMIFSIWLLAFSPQMCEHLRRYNIIPVLSDILQESVKEKVTRIILAAFRNFLEKSTERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UI12 As Substrate

Q9UI12

Site	PTM Type	Source
T16	Phosphorylation	Uniprot
K22	Ubiquitination	Uniprot
K62	Ubiquitination	Uniprot
K176	Ubiquitination	Uniprot
K313	Ubiquitination	Uniprot
K316	Ubiquitination	Uniprot
K325	Ubiquitination	Uniprot
K341	Ubiquitination	Uniprot
S351	Phosphorylation	Uniprot
K360	Ubiquitination	Uniprot
S367	Phosphorylation	Uniprot
K371	Ubiquitination	Uniprot
K374	Ubiquitination	Uniprot
K386	Ubiquitination	Uniprot
Y388	Phosphorylation	Uniprot
K396	Ubiquitination	Uniprot
Y466	Phosphorylation	Uniprot
K469	Ubiquitination	Uniprot
S483	Phosphorylation	Uniprot

Research Backgrounds

Q9UI12_VATH_HUMAN

Function:

Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.

Tissue Specificity:

Widely expressed.

Subunit Structure:

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with AP2M1. Interacts with TM9SF4 in colon cancer cells.

(Microbial infection) Interacts with HIV-1 Nef protein.

Family&Domains:

Belongs to the V-ATPase H subunit family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

References

1). Bioengineered microglia-targeted exosomes facilitate Aβ clearance via enhancing activity of microglial lysosome for promoting cognitive recovery in Alzheimer's disease. Biomaterials Advances, 2022 (PubMed: 35929330)

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V-ATPase H Antibody - #AF9227