UMP/CMP kinase Antibody - #AF9222

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Product: UMP/CMP kinase Antibody
Catalog: AF9222
Description: Rabbit polyclonal antibody to UMP/CMP kinase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 22kDa; 22kD(Calculated).
Uniprot: P30085
RRID: AB_2843412

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
UMP/CMP kinase Antibody detects endogenous levels of total UMP/CMP kinase.
RRID:
AB_2843412
Cite Format: Affinity Biosciences Cat# AF9222, RRID:AB_2843412.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CMK; CMPK 1; CMPK; cmpk1; Cytidine monophosphate (UMP CMP) kinase 1 cytosolic; Cytidine monophosphate kinase; Cytidine monophosphate UMP CMP kinase 1 cytosolic; Cytidylate kinase; dCMP kinase; Deoxycytidylate kinase; EC 2.7.4.14; KCY; KCY_HUMAN; Monophosphatase kinase; OTTHUMP00000009565; RP11 511I2.1; UCK; UMK; UMP CMP; UMP CMP kinase; UMP-CMP kinase; UMP/CMP kinase; UMP/CMPK; UMPK; Uridine monophosphate kinase; Uridine monophosphate/cytidine monophosphate kinase;

Immunogens

Immunogen:
Uniprot:

P30085(KCY_HUMAN) >>Visit HPA database.

Gene(ID):
CMPK1(51727)
Expression:
P30085 KCY_HUMAN:

Ubiquitously expressed.

Sequence:
MKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
88
Xenopus
88
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30085 As Substrate

Site PTM Type Enzyme
K26 Ubiquitination
S33 Phosphorylation
K43 Acetylation
K43 Ubiquitination
Y49 Phosphorylation
K55 Acetylation
K55 Ubiquitination
Y56 Phosphorylation
T68 Phosphorylation
S70 Phosphorylation
K86 Ubiquitination
K88 Ubiquitination
K106 Methylation
K106 Ubiquitination
T107 Phosphorylation
K150 Ubiquitination
Y166 Phosphorylation
S180 Phosphorylation

Research Backgrounds

Function:

Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Predominantly cytoplasmic, less than 15% nuclear.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Monomer.

Family&Domains:

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.

Belongs to the adenylate kinase family. UMP-CMP kinase subfamily.

Research Fields

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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