TBX2 Antibody - #AF9210

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Product: TBX2 Antibody
Catalog: AF9210
Description: Rabbit polyclonal antibody to TBX2
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 74kDa; 75kD(Calculated).
Uniprot: Q13207
RRID: AB_2843400

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TBX2 Antibody detects endogenous levels of total TBX2.
RRID:
AB_2843400
Cite Format: Affinity Biosciences Cat# AF9210, RRID:AB_2843400.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ10169; HGNC:11597; T box 2; T box transcription factor TBX2; T-box protein 2; T-box transcription factor TBX2; TBX2; TBX2_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q13207(TBX2_HUMAN) >>Visit HPA database.

Gene(ID):
TBX2(6909)
Expression:
Q13207 TBX2_HUMAN:

Expressed primarily in adult in kidney, lung, and placenta. Weak expression in heart and ovary.

Sequence:
MREPALAASAMAYHPFHAPRPADFPMSAFLAAAQPSFFPALALPPGALAKPLPDPGLAGAAAAAAAAAAAAEAGLHVSALGPHPPAAHLRSLKSLEPEDEVEDDPKVTLEAKELWDQFHKLGTEMVITKSGRRMFPPFKVRVSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPEMPKRMYIHPDSPATGEQWMAKPVAFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRANDILKLPYSTFRTYVFPETDFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRREKRKQLTLPSLRLYEEHCKPERDGAESDASSCDPPPAREPPTSPGAAPSPLRLHRARAEEKSCAADSDPEPERLSEERAGAPLGRSPAPDSASPTRLTEPERARERRSPERGKEPAESGGDGPFGLRSLEKERAEARRKDEGRKEAAEGKEQGLAPLVVQTDSASPLGAGHLPGLAFSSHLHGQQFFGPLGAGQPLFLHPGQFTMGPGAFSAMGMGHLLASVAGGGNGGGGGPGTAAGLDAGGLGPAASAASTAAPFPFHLSQHMLASQGIPMPTFGGLFPYPYTYMAAAAAAASALPATSAAAAAAAAAGSLSRSPFLGSARPRLRFSPYQIPVTIPPSTSLLTTGLASEGSKAAGGNSREPSPLPELALRKVGAPSRGALSPSGSAKEAANELQSIQRLVSGLESQRALSPGRESPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
67
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13207 As Substrate

Site PTM Type Enzyme
S94 Phosphorylation
Y165 Phosphorylation
K210 Ubiquitination
Y247 Phosphorylation
T252 Phosphorylation
Y253 Phosphorylation
T258 Phosphorylation
K275 Ubiquitination
K283 Ubiquitination
K295 Ubiquitination
S320 Phosphorylation
S323 Phosphorylation
S324 Phosphorylation
S336 Phosphorylation
S342 Phosphorylation
S360 Phosphorylation
S368 Phosphorylation
S379 Phosphorylation
S384 Phosphorylation
S386 Phosphorylation
T391 Phosphorylation
S401 Phosphorylation
S635 Phosphorylation
T638 Phosphorylation
S643 Phosphorylation
S646 Phosphorylation
S653 Phosphorylation
S657 Phosphorylation
S676 Phosphorylation
S705 Phosphorylation
S710 Phosphorylation

Research Backgrounds

Function:

Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence. May be required for cardiac atrioventricular canal formation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed primarily in adult in kidney, lung, and placenta. Weak expression in heart and ovary.

Subunit Structure:

Interacts with PML (isoform-PML-2, isoform PML-3 and isoform PML-4).

Family&Domains:

The repression domain 1 (RD1) is necessary for its interaction with PML.

Restrictive clause

 

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For Research Use Only.
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