StIp1 Antibody | Affinity Biosciences

IHC
Cites

1/3

AF9204 at 1/100 staining Human kidney tissue by IHC-P.

FIGURE 1 STIP1 expression increases firstly and then decreases in rats after SCII treatment.

FIGURE 3 STIP1 ameliorates ischaemia/reperfusion-induced inflammation in rat spinal cord.

Product:	StIp1 Antibody
Catalog:	AF9204
Description:	Rabbit polyclonal antibody to StIp1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	93kDa; 93kD(Calculated).
Uniprot:	Q6IA86
RRID:	AB_2843394

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

StIp1 Antibody detects endogenous levels of total StIp1.

RRID:

AB_2843394
Cite Format: Affinity Biosciences Cat# AF9204, RRID:AB_2843394.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AU023723; Elongation protein 2 homolog (S. cerevisiae); Elongator acetyltransferase complex subunit 2; Elongator complex protein 2; elongator protein 2; ELP2; ELP2_HUMAN; Epl2; FLJ10879; SHINC 2; SHINC-2; SHINC2; signal transducer and activator of transcription 3 interacting protein 1; signal transducer and activator of transcription interacting protein 1; STAT3 interacting protein 1; Stat3 interacting protein; STAT3-interacting protein 1; STATIP 1; STATIP1; STATIP1 protein; StIP; StIP1;

Immunogens

Immunogen:

Uniprot:

Q6IA86(ELP2_HUMAN) >>Visit HPA database.

Gene(ID):

ELP2(55250)

Sequence:

Q6IA86 ELP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVAPVLETSHVFCCPNRVRGVLNWSSGPRGLLAFGTSCSVVLYDPLKRVVVTNLNGHTARVNCIQWICKQDGSPSTELVSGGSDNQVIHWEIEDNQLLKAVHLQGHEGPVYAVHAVYQRRTSDPALCTLIVSAAADSAVRLWSKKGPEVMCLQTLNFGNGFALALCLSFLPNTDVPILACGNDDCRIHIFAQQNDQFQKVLSLCGHEDWIRGVEWAAFGRDLFLASCSQDCLIRIWKLYIKSTSLETQDDDNIRLKENTFTIENESVKIAFAVTLETVLAGHENWVNAVHWQPVFYKDGVLQQPVRLLSASMDKTMILWAPDEESGVWLEQVRVGEVGGNTLGFYDCQFNEDGSMIIAHAFHGALHLWKQNTVNPREWTPEIVISGHFDGVQDLVWDPEGEFIITVGTDQTTRLFAPWKRKDQSQVTWHEIARPQIHGYDLKCLAMINRFQFVSGADEKVLRVFSAPRNFVENFCAITGQSLNHVLCNQDSDLPEGATVPALGLSNKAVFQGDIASQPSDEEELLTSTGFEYQQVAFQPSILTEPPTEDHLLQNTLWPEVQKLYGHGYEIFCVTCNSSKTLLASACKAAKKEHAAIILWNTTSWKQVQNLVFHSLTVTQMAFSPNEKFLLAVSRDRTWSLWKKQDTISPEFEPVFSLFAFTNKITSVHSRIIWSCDWSPDSKYFFTGSRDKKVVVWGECDSTDDCIEHNIGPCSSVLDVGGAVTAVSVCPVLHPSQRYVVAVGLECGKICLYTWKKTDQVPEINDWTHCVETSQSQSHTLAIRKLCWKNCSGKTEQKEAEGAEWLHFASCGEDHTVKIHRVNKCAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Pig

Chicken

Xenopus

Horse

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6IA86 As Substrate

Q6IA86

Site	PTM Type	Source
Y117	Phosphorylation	Uniprot
K241	Ubiquitination	Uniprot
K256	Acetylation	Uniprot
K256	Ubiquitination	Uniprot
K314	Ubiquitination	Uniprot
K419	Ubiquitination	Uniprot
K421	Ubiquitination	Uniprot
K442	Ubiquitination	Uniprot
K459	Ubiquitination	Uniprot
S519	Phosphorylation	Uniprot
Y532	Phosphorylation	Uniprot
K579	Ubiquitination	Uniprot
K587	Ubiquitination	Uniprot
Y752	Phosphorylation	Uniprot
K756	Ubiquitination	Uniprot
K788	Ubiquitination	Uniprot
K797	Ubiquitination	Uniprot
K817	Ubiquitination	Uniprot
K823	Ubiquitination	Uniprot

Research Backgrounds

Q6IA86_ELP2_HUMAN

Function:

Component of the RNA polymerase II elongator complex, a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation. The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.

Subcellular Location:

Cytoplasm. Nucleus.

Subunit Structure:

Component of the RNA polymerase II elongator complex (Elongator), which consists of ELP1, STIP1/ELP2, ELP3, ELP4, ELP5 and ELP6. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with STAT3 and JAKs (By similarity).

Family&Domains:

Belongs to the WD repeat ELP2 family.

References

1). Stress-induced phosphoprotein 1 restrains spinal cord ischaemia-reperfusion injury by modulating NF-κB signalling. Journal of Cellular and Molecular Medicine, 2021 (PubMed: 34734476) [IF=5.3]

Application: WB Species: Rat Sample: spinal cord

FIGURE 1 STIP1 expression increases firstly and then decreases in rats after SCII treatment. A, The rats undergo ischaemia by clamping abdominal aorta for 1 h and reperfusion for 24 h; B, Western blotting detects STIP1 expression in rat spinal cord specimens in absence or presence of SCII treatment; C, Immunohistochemical staining detects STIP1 expression in rat spinal cord specimens. The brown represents STIP1, and the STIP1-postive cells are indicated by arrows. Scale bar =50 μm. SCII, spinal cord ischaemia-reperfusion injury; STIP1, stress-induced phosphoprotein 1. **p < 0.01, ***p < 0.001 versus the Sham group

Application: IF/ICC Species: Rat Sample: spinal cord

FIGURE 3 STIP1 ameliorates ischaemia/reperfusion-induced inflammation in rat spinal cord. A, Immunohistochemical staining determines the expression and location of Iba-1 and STIP1. The arrows indicate co-location of STIP1 and Iba-1. Scale bar =50 μm; B, ELISA detects TNF-α and IL-6 secretion in rat spinal cord specimens following SCII treatment and STIP1 overexpression; C, Western blotting determines IκBβ expression in rat spinal cord specimens; D, Western blotting determines the cytoplasmic and nuclear expression of NF-κB p65. NF-κB, nuclear factor kappa B; SCII, spinal cord ischaemia-reperfusion injury; STIP1, stress-induced phosphoprotein 1; TNF, tumour necrosis factor. ***p < 0.001 versus the Sham group; #p < 0.05, ###p < 0.001 versus the SCII + LV group

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StIp1 Antibody - #AF9204