Sp3/4 Antibody - #AF9200
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF9200, RRID:AB_2843390.
Fold/Unfold
D130027J01Rik; DKFZp686O1631; GC binding transcription factor Sp 3; GC binding transcription factor Sp3; MGC105187; OTTMUSP00000014207; SP 3; Sp 3 transcription factor; SP3; Sp3 transcription factor; SP3_HUMAN; Specificity protein 3; SPR 2; SPR-2; SPR2; Transcription factor SP 3; Transcription factor Sp3; HF1B; MGC130008; MGC130009; Sp4; SP4_HUMAN; SPR-1; SPR1; Transcription factor Sp4;
Immunogens
- Q02447 SP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTAPEKPVKQEEMAALDVDSGGGGGGGGGHGEYLQQQQQHGNGAVAAAAAAQDTQPSPLALLAATCSKIGPPSPGDDEEEAAAAAGAPAAAGATGDLASAQLGGAPNRWEVLSATPTTIKDEAGNLVQIPSAATSSGQYVLPLQNLQNQQIFSVAPGSDSSNGTVSSVQYQVIPQIQSADGQQVQIGFTGSSDNGGINQESSQIQIIPGSNQTLLASGTPSANIQNLIPQTGQVQVQGVAIGGSSFPGQTQVVANVPLGLPGNITFVPINSVDLDSLGLSGSSQTMTAGINADGHLINTGQAMDSSDNSERTGERVSPDINETNTDTDLFVPTSSSSQLPVTIDSTGILQQNTNSLTTSSGQVHSSDLQGNYIQSPVSEETQAQNIQVSTAQPVVQHLQLQESQQPTSQAQIVQGITPQTIHGVQASGQNISQQALQNLQLQLNPGTFLIQAQTVTPSGQVTWQTFQVQGVQNLQNLQIQNTAAQQITLTPVQTLTLGQVAAGGAFTSTPVSLSTGQLPNLQTVTVNSIDSAGIQLHPGENADSPADIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGTVNTSATSNQDILTNTEIPLQLVTVSGNETME
- Q02446 SP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDQKKEEEEEAAAAAAMATEGGKTSEPENNNKKPKTSGSQDSQPSPLALLAATCSKIGTPGENQATGQQQIIIDPSQGLVQLQNQPQQLELVTTQLAGNAWQLVASTPPASKENNVSQPASSSSSSSSSNNGSASPTKTKSGNSSTPGQFQVIQVQNPSGSVQYQVIPQLQTVEGQQIQINPTSSSSLQDLQGQIQLISAGNNQAILTAANRTASGNILAQNLANQTVPVQIRPGVSIPLQLQTLPGTQAQVVTTLPINIGGVTLALPVINNVAAGGGTGQVGQPAATADSGTSNGNQLVSTPTNTTTSASTMPESPSSSTTCTTTASTSLTSSDTLVSSADTGQYASTSASSSERTIEESQTPAATESEAQSSSQLQPNGMQNAQDQSNSLQQVQIVGQPILQQIQIQQPQQQIIQAIPPQSFQLQSGQTIQTIQQQPLQNVQLQAVNPTQVLIRAPTLTPSGQISWQTVQVQNIQSLSNLQVQNAGLSQQLTITPVSSSGGTTLAQIAPVAVAGAPITLNTAQLASVPNLQTVSVANLGAAGVQVQGVPVTITSVAGQQQGQDGVKVQQATIAPVTVAVGGIANATIGAVSPDQLTQVHLQQGQQTSDQEVQPGKRLRRVACSCPNCREGEGRGSNEPGKKKQHICHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSVTEVLGSPRIVTVAAISQDSNPATPNVSTNMEEF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q02447/Q02446 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T65 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| T115 | Phosphorylation | Uniprot | |
| K120 | Sumoylation | Uniprot | |
| K551 | Acetylation | Uniprot | |
| K551 | Sumoylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| K593 | Acetylation | Uniprot | |
| K593 | Ubiquitination | Uniprot | |
| T601 | Phosphorylation | Uniprot | |
| K606 | Ubiquitination | Uniprot | |
| R611 | Methylation | Uniprot | |
| S646 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| T674 | Phosphorylation | Uniprot | |
| T676 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T20 | Phosphorylation | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| R457 | Methylation | Uniprot | |
| T589 | Phosphorylation | Uniprot | |
| K643 | Sumoylation | Uniprot | |
| T672 | Phosphorylation | Uniprot | |
| S691 | Phosphorylation | Uniprot | |
| T700 | Phosphorylation | Uniprot | |
| T702 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| S767 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Uniprot | |
| T774 | Phosphorylation | Uniprot |
Research Backgrounds
Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
Not glycosylated.
Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. In vitro, C(18)-ceramides, but not C(16)-ceramides, increase the interaction of HDAC1 with SP3 and enhance the deacetylation of SP3 and the subsequent repression of the TERT promoter.
Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity.
Nucleus. Nucleus>PML body.
Note: Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.
Ubiquitously expressed.
Interacts with HLTF; the interaction may be required for basal transcriptional activity of HLTF. Interacts with HDAC1; the interaction deacetylates SP3 and regulates its transcriptional activity. Interacts with HDAC2 (preferably the CK2-phosphorylated form); the interaction deacetylates SP3 and regulates its transcriptional activity. Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a.
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Binds to GT and GC boxes promoters elements. Probable transcriptional activator.
Nucleus.
Abundant in brain.
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. In SP4, the motif is inactive.
Belongs to the Sp1 C2H2-type zinc-finger protein family.
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