Septin 8 Antibody - #AF9197

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Product: Septin 8 Antibody
Catalog: AF9197
Description: Rabbit polyclonal antibody to Septin 8
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50kDa; 56kD(Calculated).
Uniprot: Q92599
RRID: AB_2843387

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Septin 8 Antibody detects endogenous levels of total Septin 8.
RRID:
AB_2843387
Cite Format: Affinity Biosciences Cat# AF9197, RRID:AB_2843387.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA0202; OTTHUMP00000211755; SEP 2; SEP2; SEPT 8; SEPT8; Septin8;

Immunogens

Immunogen:
Uniprot:

Q92599(SEPT8_HUMAN) >>Visit HPA database.

Gene(ID):
SEPT8(23176)
Expression:
Q92599 SEPT8_HUMAN:

Widely expressed, including in brain, heart and platelets; most abundant in aorta. Isoform 2 is expressed at low levels in specific brain areas, such as occipital pole, frontal lobe, temporal lobe and putamen. Isoform 1 and 3 are highly expressed in specific brain areas, such as occipital pole, frontal lobe, temporal lobe and putamen. Isoform 2 is highly expressed in prostate, testis and ovary. Isoform 1 and isoform 3 are expressed at low levels in prostate, testis and ovary.

Sequence:
MAATDLERFSNAEPEPRSLSLGGHVGFDSLPDQLVSKSVTQGFSFNILCVGETGIGKSTLMNTLFNTTFETEEASHHEACVRLRPQTYDLQESNVQLKLTIVDAVGFGDQINKDESYRPIVDYIDAQFENYLQEELKIRRSLFDYHDTRIHVCLYFITPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADTISKSELHKFKIKIMGELVSNGVQIYQFPTDDEAVAEINAVMNAHLPFAVVGSTEEVKVGNKLVRARQYPWGVVQVENENHCDFVKLREMLIRVNMEDLREQTHSRHYELYRRCKLEEMGFQDSDGDSQPFSLQETYEAKRKEFLSELQRKEEEMRQMFVNKVKETELELKEKERELHEKFEHLKRVHQEEKRKVEEKRRELEEETNAFNRRKAAVEALQSQALHATSQQPLRKDKDKKNRSDIGAHQPGMSLSSSKVMMTKASVEPLNCSSWWPAIQCCSCLVRDATWREGFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
67
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92599 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T4 Phosphorylation
S10 Phosphorylation
R17 Methylation
S20 Phosphorylation
S29 Phosphorylation
S36 Phosphorylation
T100 Phosphorylation
S141 Phosphorylation
S163 Phosphorylation Q8IW41 (MAPKAPK5)
K173 Ubiquitination
K178 Ubiquitination
S192 Phosphorylation Q8IW41 (MAPKAPK5)
K193 Acetylation
K193 Ubiquitination
K198 Acetylation
K198 Ubiquitination
K200 Acetylation
K251 Ubiquitination
Y297 Phosphorylation
Y300 Phosphorylation
K304 Ubiquitination
S313 Phosphorylation
S321 Phosphorylation
Y326 Phosphorylation
K329 Ubiquitination
K331 Ubiquitination
K340 Ubiquitination
K351 Ubiquitination
K353 Ubiquitination
K360 Ubiquitination
K369 Ubiquitination
K381 Acetylation
K387 Acetylation
K402 Ubiquitination
T416 Phosphorylation

Research Backgrounds

Function:

Filament-forming cytoskeletal GTPase (By similarity). May play a role in platelet secretion. Seems to participate in the process of SNARE complex formation in synaptic vesicles (By similarity).

Stabilizes BACE1 protein levels and promotes the sorting and accumulation of BACE1 to the recycling or endosomal compartments, modulating the beta-amyloidogenic processing of APP.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Cell junction>Synapse. Cell projection>Axon. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane. Cell junction>Synapse>Presynapse.
Note: Expressed in axons of immature neurons, localizes to synapses in mature neurons (By similarity). In platelets, found in areas surrounding alpha-granules (PubMed:15116257).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, including in brain, heart and platelets; most abundant in aorta. Isoform 2 is expressed at low levels in specific brain areas, such as occipital pole, frontal lobe, temporal lobe and putamen. Isoform 1 and 3 are highly expressed in specific brain areas, such as occipital pole, frontal lobe, temporal lobe and putamen. Isoform 2 is highly expressed in prostate, testis and ovary. Isoform 1 and isoform 3 are expressed at low levels in prostate, testis and ovary.

Subunit Structure:

Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity). Interacts with CDK14. Interacts with SEPTIN5. Interacts with SEPTIN7 (By similarity). Interacts with SEPTIN4. Interacts with VAMP2; the interaction inhibits interaction of VAMP2 with SYP (By similarity). Interacts with STX1A (By similarity).

Family&Domains:

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

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