Septin 1 Antibody - #AF9196

(1)  
Product: Septin 1 Antibody
Catalog: AF9196
Description: Rabbit polyclonal antibody to Septin 1
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 41kDa; 42kD(Calculated).
Uniprot: Q8WYJ6
RRID: AB_2843386

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(83%)
Clonality:
Polyclonal
Specificity:
Septin 1 Antibody detects endogenous levels of total Septin 1.
RRID:
AB_2843386
Cite Format: Affinity Biosciences Cat# AF9196, RRID:AB_2843386.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DIFF6; Differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase); LARP; MGC20394; Peanut like protein 3; Peanut-like protein 3; PNUTL3; SEP1; SEPT1; SEPT1_HUMAN; Septin 1; Septin-1; Serologically defined breast cancer antigen NY BR 24; Serologically defined breast cancer antigen NY-BR-24;

Immunogens

Immunogen:
Uniprot:

Q8WYJ6(SEPT1_HUMAN) >>Visit HPA database.

Gene(ID):
SEPT1(1731)
Expression:
Q8WYJ6 SEPT1_HUMAN:

Expressed at high levels in lymphoid and hematopoietic tissues.

Sequence:
MDKEYVGFAALPNQLHRKSVKKGFDFTLMVAGESGLGKSTLINSLFLTNLYEDRQVPEASARLTQTLAIERRGVEIEEGGVKVKLTLVDTPGFGDSVDCSDCWLPVVKFIEEQFEQYLRDESGLNRKNIQDSRVHCCLYFISPFGRGLRPLDVAFLRAVHEKVNIIPVIGKADALMPQETQALKQKIRDQLKEEEIHIYQFPECDSDEDEDFKRQDAEMKESIPFAVVGSCEVVRDGGNRPVRGRRYSWGTVEVENPHHCDFLNLRRMLVQTHLQDLKEVTHDLLYEGYRARCLQSLARPGARDRASRSKLSRQSATEIPLPMLPLADTEKLIREKDEELRRMQEMLEKMQAQMQQSQAQGEQSDAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
83
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WYJ6 As Substrate

Site PTM Type Enzyme
K3 Ubiquitination
Y5 Phosphorylation
K18 Ubiquitination
S19 Phosphorylation
K22 Ubiquitination
T48 Phosphorylation
Y51 Phosphorylation
S60 Phosphorylation
K82 Ubiquitination
K84 Ubiquitination
K127 Ubiquitination
S132 Phosphorylation
K162 Ubiquitination
K171 Ubiquitination
K184 Ubiquitination
K186 Ubiquitination
Y199 Phosphorylation
S206 Phosphorylation
K213 Ubiquitination
K220 Ubiquitination
R240 Methylation
Y247 Phosphorylation
S248 Phosphorylation Q96GD4 (AURKB)
T251 Phosphorylation
K278 Ubiquitination
Y286 Phosphorylation
Y289 Phosphorylation
S307 Phosphorylation Q96GD4 (AURKB)
S315 Phosphorylation Q96GD4 (AURKB)
T317 Phosphorylation
K331 Ubiquitination
K336 Ubiquitination
K349 Ubiquitination

Research Backgrounds

Function:

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential).

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Midbody.
Note: Remains at the centrosomes and the nearby microtubules throughout mitosis. Localizes to the midbody during cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in lymphoid and hematopoietic tissues.

Subunit Structure:

Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity). Interacts with AURKB.

Family&Domains:

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

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