Separase Antibody - #AF9195

(2)  
Product: Separase Antibody
Catalog: AF9195
Description: Rabbit polyclonal antibody to Separase
Application: WB IF/ICC
Reactivity: Human
Prediction: Sheep, Rabbit, Dog
Mol.Wt.: 230kDa; 233kD(Calculated).
Uniprot: Q14674
RRID: AB_2843385

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Sheep(80%), Rabbit(90%), Dog(90%)
Clonality:
Polyclonal
Specificity:
Separase Antibody detects endogenous levels of total Separase.
RRID:
AB_2843385
Cite Format: Affinity Biosciences Cat# AF9195, RRID:AB_2843385.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Caspase like protein ESPL1; Caspase-like protein ESPL1; ESP 1; ESP1; ESPL 1; Espl1; ESPL1_HUMAN; Extra spindle poles like 1; Extra spindle poles like 1 protein; Extra spindle poles-like 1 protein; FLJ46492; KIAA0165; Separase; Separin; Similar to fission yeast cut1and gene; SSE;

Immunogens

Immunogen:
Uniprot:

Q14674(ESPL1_HUMAN) >>Visit HPA database.

Gene(ID):
ESPL1(9700)
Sequence:
MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Dog
90
Sheep
80
Pig
0
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14674 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
K64 Ubiquitination
Y140 Phosphorylation
K153 Ubiquitination
K277 Ubiquitination
K299 Ubiquitination
K313 Ubiquitination
K324 Ubiquitination
K491 Ubiquitination
K562 Acetylation
K562 Ubiquitination
K567 Ubiquitination
K593 Ubiquitination
Y719 Phosphorylation
K722 Ubiquitination
K755 Ubiquitination
K760 Ubiquitination
S810 Phosphorylation
Y973 Phosphorylation
K1008 Ubiquitination
K1016 Ubiquitination
K1020 Ubiquitination
S1073 Phosphorylation
K1083 Ubiquitination
T1111 Phosphorylation
S1122 Phosphorylation
T1123 Phosphorylation
S1126 Phosphorylation P06493 (CDK1) , P28482 (MAPK1)
S1153 Phosphorylation
K1189 Ubiquitination
T1210 Phosphorylation
K1252 Ubiquitination
S1305 Phosphorylation
K1309 Ubiquitination
S1316 Phosphorylation
K1322 Ubiquitination
S1325 Phosphorylation
K1336 Ubiquitination
R1341 Methylation
T1346 Phosphorylation
T1363 Phosphorylation P53350 (PLK1)
S1396 Phosphorylation
S1399 Phosphorylation P53350 (PLK1)
S1444 Phosphorylation
S1455 Phosphorylation
S1475 Phosphorylation
S1501 Phosphorylation P17612 (PRKACA) , Q9UQM7 (CAMK2A)
S1508 Phosphorylation
S1513 Phosphorylation
K1518 Ubiquitination
S1528 Phosphorylation
S1538 Phosphorylation
S1539 Phosphorylation
S1545 Phosphorylation
S1552 Phosphorylation
Y1614 Phosphorylation
S1649 Phosphorylation
S1660 Phosphorylation
K1692 Ubiquitination
K1732 Ubiquitination
K1747 Ubiquitination
S1752 Phosphorylation
K1762 Ubiquitination
S1768 Phosphorylation
S1769 Phosphorylation
T1771 Phosphorylation
K1796 Ubiquitination
K1803 Ubiquitination
S1820 Phosphorylation
K1832 Ubiquitination
T1884 Phosphorylation
S1887 Phosphorylation
S1889 Phosphorylation
K1901 Ubiquitination
K1994 Ubiquitination
K2103 Ubiquitination
Y2104 Phosphorylation
S2118 Phosphorylation

Research Backgrounds

Function:

Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.

PTMs:

Autocleaves. This function, which is not essential for its protease activity, is unknown.

Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PTTG1. Interacts with RAD21.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

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